Microwave radiation accelerates trypsin-catalyzed peptide hydrolysis at constant bulk temperature

Microwave Irradiation of PC3 Cells at Constant Culture Temperature Alters the Incorporation of BODIPY into Cells and Reduction of MTT

“…In order to maintain the culture temperature, the cultures were irradiated with microwave while the temperature, as measured in real-time by a fibre optic temperature probe (Figure 1), was maintained at 37 AE 2 8C through cooling. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 Maintenance of temperature of PC3 cultures exposed to microwave As demonstrated by us previously, [7,8] our system maintains temperature of the cell suspension in the CEM microwave reactor within 1 or 2 degrees of the setpoint, here 37 8C. In the present study, temperatures of all the PC3 cell suspensions were continually monitored over the duration of the experiments.…”

“…As demonstrated by us previously,, our system maintains temperature of the cell suspension in the CEM microwave reactor within 1 or 2 degrees of the setpoint, here 37 °C. In the present study, temperatures of all the PC3 cell suspensions were continually monitored over the duration of the experiments.…”

“…We previously demonstrated effects of microwave irradiation under constant temperature conditions on certain protein activities . The enzymatic activity of trypsin is significantly increased by exposure to 2.45 GHz microwave irradiation while maintaining temperature of the reaction mixture via simultaneous cooling.…”

“…We previously demonstrated effects of microwave irradiation under constant temperature conditions on certain protein activities. [7] The enzymatic activity of trypsin is significantly increased by exposure to 2.45 GHz microwave irradiation while maintaining temperature of the reaction mixture via simultaneous cooling. Interestingly though, the enzymatic activity of amylase and phosphatase are not affected by exposure to microwave under the same conditions, [8] indicating specificity of the effect.…”

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

“…Because of the biotechnological importance of keratinases, several studies have been performed to improve the activity and to evaluate the conformational change of enzymes in general, when exposed to different reaction systems, including ultrasonic probe and bath and microwave (Ma et al 2011;Jin et al 2015;Mazinani, DeLong and Yan 2015). Simple and e cient heating technologies increase enzyme activity and conformational change (Ma et al 2011); residue pretreatment techniques using an ultrasonic probe (Azmi, Idris and Yusof 2018), makes the structures more accessible to microbial and enzymatic attack (Yusof and Ashokkumar 2015).…”