Midgut transcriptome analysis of <i>Clostera anachoreta</i> treated with lethal and sublethal Cry1Ac protoxin

Liu, Jie; Wang, Liucheng; Zhou, Guozhong; Gao, Suhong; Tianhua, Sun; Liu, Junxia; Bao-jia, Gao

doi:10.1002/arch.21638

Cited by 7 publications

(5 citation statements)

References 54 publications

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“…ABC transporters play an important role in Bt‐toxin poisoning and resistance in insect (Atsumi et al, 2012; Gahan et al, 2010; Tay et al, 2015). A large number of research found that ABC transporters changed their expression after Bt‐toxin treatment (Lei et al, 2014; Liu et al, 2020). Unsurprisingly, it was found that seven ABC transporters were significantly changed between Cry1Ac treatment group and control group, including ABCA2 (BMSK0007991), ABCA3 (BMSK0010069), ABCC4 (BMSK0008667), ABCF1 (BMSK0008549), ABCF4 (BMSK0009342), ABCG4 (BMSK0006983), and ABCG1 (BMSK0006984)).…”

Section: Resultsmentioning

confidence: 99%

“…DEGs between Cry1Ac susceptible and resistance strains of Helicoverpa armigera were significantly enriched in ABC transporter pathway (Wei et al, 2018). RNA‐Seq data showed that ABCC4 and ABCG1 can be activated by Cry1Ac in Clostera anachoreta (Liu et al, 2020). In our data, it was observed that the expression of ABCA2, ABCA3, ABCC4, and ABCG4 were significantly upregulated in Cry1Ac treatment group.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome reveal the response to Cry1Ac toxin in susceptible Bombyx mori

Qin

Zhang

Sun

et al. 2021

Arch Insect Biochem Physiol

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Bombyx mori as a representative in Lepidoptera is an important economic insect in agriculture production. Bacillus thuringiensis (Bt) is a bacterial pathogen in silkworm production. Understanding how silkworm respond to Bt‐toxin can provide guidance to cultivate resistant silkworm strains. Cry1Ac is one type of Bt‐toxin. In current research, Dazao, a susceptible B. mori strain to Bt‐toxin, was treated by Cry1Ac toxin and compared its transcriptome with untreated samples. This analysis detected 1234 differentially expressed genes (DEGs). Gene Ontology, KEGG, and UniProt keyword enrichment analysis showed that DEGs include ATP‐binding cassette (ABC) transporter, stress response, cuticle, and protein synthesis, and folding process. Five ABC genes were upregulated after Cry1Ac treatment including ABCA2, ABCA3, and ABCC4. They are also known as the transporters of Bt‐toxin in lepidopteran insect. Expression of cuticle proteins was significantly increased at 6 h after Cry1Ac treatment. Sex‐specific storage‐proteins and heat shock protein were also upregulated in Cry1Ac treated samples. Our data provide an expression profile about the response of Cry1Ac toxin in susceptible B. mori strain.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptome reveal the response to Cry1Ac toxin in susceptible Bombyx mori

Qin

Zhang

Sun

et al. 2021

Arch Insect Biochem Physiol

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“…In that sense, a previous study provided insight into a novel ABC transporter gene PxABCH1 in diamondback moth, Plutella xylostella (L.), and revealed it as an excellent target for RNAi‐based insect pest control and resistance management (Guo et al, 2015). A Cadherin‐like protein is also a potential receptor of Bt toxins, and its down‐regulated expression or mutation is one of the main causes of resistance in pests (Liu et al, 2019). Additional work is necessary to unravel whether some of the outlier SNP markers detected in this study indeed have biological significance (Barrett & Hoekstra, 2011).…”

Section: Discussionmentioning

confidence: 99%

Unraveling the population structure of the sugarcane borer,Diatraea saccharalis, in Argentina

Fogliata

Perera

Alves‐Pereira

et al. 2022

Entomologia Exp Applicata

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Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) is one of the major lepidopteran pests throughout the Western Hemisphere. In Argentina, it causes significant losses mainly in sugarcane (Saccharum officinarum L.) and corn (maize, Zea mays L.) (both Poaceae). Previous studies determined the existence of reproductive incompatibilities between populations from Buenos Aires and Tucumán from different host plants; however, the genetic basis of this incompatibility is still unknown. As the effectiveness of pest control programs mainly depends on strategies that minimize the risk of favoring insecticide‐resistant genotypes, estimating the level of mating between genotypes is important to monitor and manage resistance. The aim of this study was to characterize the genetic structure of D. saccharalis populations collected from various hosts and regions in Argentina, comparing with those from Brazil, through cytochrome c oxidase subunit I (COI) analysis and next‐generation sequencing (NGS). The COI analysis showed a haplotypic diversity of 0.8 and a nucleotide diversity of 0.0058. Analysis of molecular variance revealed greater variation within populations than among them. The 17 haplotypes detected were linked in a single parsimony network that did not reveal clusters based on geography or host plants. In total, 4549 single nucleotide polymorphism markers (SNPs) were obtained through NGS. Out of the 2349 outlier loci, 84 showed similarities with previously characterized proteins. The coefficient of inbreeding showed evidence of random matings as well as some degree of selection. The fixation index values showed high genetic variation between Argentinean and Brazilian populations; however, there was no clear trend based on distance or hosts. Similarly, the discriminant analysis of principal components revealed three separate groups: one Brazilian and two Argentinean. Results generated important clarification of the population genetic structure of D. saccharalis in South America, which provides information about routes of dispersal and the development of suitable management strategies.

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“…We found the LC50 dose of Bt infection could suppress the humoral immune response in the third instar larvae of Plutella xylostella [ 80 ]. Growing evidence suggests that Bt-induced immunity is a dose-dependent effect [ 88 , 89 , 90 , 91 ].…”

Section: The Host Immune System In Response To Bt Infectionmentioning

confidence: 99%

The Tripartite Interaction of Host Immunity–Bacillus thuringiensis Infection–Gut Microbiota

Mandal

et al. 2020

Toxins

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Bacillus thuringiensis (Bt) is an important cosmopolitan bacterial entomopathogen, which produces various protein toxins that have been expressed in transgenic crops. The evolved molecular interaction between the insect immune system and gut microbiota is changed during the Bt infection process. The host immune response, such as the expression of induced antimicrobial peptides (AMPs), the melanization response, and the production of reactive oxygen species (ROS), varies with different doses of Bt infection. Moreover, B. thuringiensis infection changes the abundance and structural composition of the intestinal bacteria community. The activated immune response, together with dysbiosis of the gut microbiota, also has an important effect on Bt pathogenicity and insect resistance to Bt. In this review, we attempt to clarify this tripartite interaction of host immunity, Bt infection, and gut microbiota, especially the important role of key immune regulators and symbiotic bacteria in the Bt killing activity. Increasing the effectiveness of biocontrol agents by interfering with insect resistance and controlling symbiotic bacteria can be important steps for the successful application of microbial biopesticides.

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Midgut transcriptome analysis of Clostera anachoreta treated with lethal and sublethal Cry1Ac protoxin

Cited by 7 publications

References 54 publications

Transcriptome reveal the response to Cry1Ac toxin in susceptible Bombyx mori

Transcriptome reveal the response to Cry1Ac toxin in susceptible Bombyx mori

Unraveling the population structure of the sugarcane borer,Diatraea saccharalis, in Argentina

The Tripartite Interaction of Host Immunity–Bacillus thuringiensis Infection–Gut Microbiota

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