MIG12 is involved in the LXR activation-mediated induction of the polymerization of mammalian acetyl-CoA carboxylase

Izumi, Akiko; Hiraguchi, Haruka; Kodaka, Manami; Ikeuchi, Emina; Narita, Junko; Kobayashi, Riko; Mu, Yu; Suzuki, Takahiro; Yamamoto, Yuji; Sato, Ryuichiro; Inoue, Jun

doi:10.1016/j.bbrc.2021.06.040

Cited by 4 publications

(5 citation statements)

References 22 publications

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“…Little has been reported on the transcriptional regulation of MID1IP1 expression. So far, only carbohydrate response element-binding protein, a transcriptional factor, and liver X receptor, a nuclear receptor, were reported to promote the expression of MID1IP1 in beta cells and HCC cells, respectively 19,23 . However, neither of these are significantly correlated with MID1IP1 expression in HCC samples within the TCGA cohort (Supplemental Figure 7b, http://links.lww.com/HEP/D711).…”

Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge, this is the first study to comprehensively characterize the implications of MID1IP1 as a potent tumor-promoting gene in cancers. Previous studies of MID1IP1 have been limited to its implications in metabolic pathways such as lipogenesis and glucose metabolism in various cell types including liver cells 19,20 . Although these pathways are important in cancer cell metabolism, its direct relevance and functional role on tumor development and progression were not clearly elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies of MID1IP1 have been limited to its implications in metabolic pathways such as lipogenesis and glucose metabolism in various cell types including liver cells. 19 , 20 Although these pathways are important in cancer cell metabolism, its direct relevance and functional role on tumor development and progression were not clearly elucidated. Scattered and brief studies touching on the relevance of MID1IP1 in cancer remain inconclusive on its functional role in tumors.…”

Section: Discussionmentioning

confidence: 99%

“…So far, only carbohydrate response element-binding protein, a transcriptional factor, and liver X receptor, a nuclear receptor, were reported to promote the expression of MID1IP1 in beta cells and HCC cells, respectively. 19 , 23 However, neither of these are significantly correlated with MID1IP1 expression in HCC samples within the TCGA cohort (Supplemental Figure 7b, http://links.lww.com/HEP/D711 ). We identified the transcriptional regulatory role of SP1 on MID1IP1 promoter as the predominant upstream regulator driving its overexpression in human HCCs.…”

Section: Discussionmentioning

confidence: 99%

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Midline 1 interacting protein 1 promotes cancer metastasis through FOS-like 1-mediated matrix metalloproteinase 9 signaling in HCC

et al. 2023

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Background and Aims: Understanding the mechanisms of HCC progression and metastasis is crucial to improve early diagnosis and treatment. This study aimed to identify key molecular targets involved in HCC metastasis. Approach and Results: Using whole-transcriptome sequencing of patients’ HCCs, we identified and validated midline 1 interacting protein 1 (MID1IP1) as one of the most significantly upregulated genes in metastatic HCCs, suggesting its potential role in HCC metastasis. Clinicopathological correlation demonstrated that MID1IP1 upregulation significantly correlated with more aggressive tumor phenotypes and poorer patient overall survival rates. Functionally, overexpression of MID1IP1 significantly promoted the migratory and invasive abilities and enhanced the sphere-forming ability and expression of cancer stemness-related genes of HCC cells, whereas its stable knockdown abrogated these effects. Perturbation of MID1IP1 led to significant tumor shrinkage and reduced pulmonary metastases in an orthotopic liver injection mouse model and reduced pulmonary metastases in a tail-vein injection model in vivo. Mechanistically, SP1 transcriptional factor was found to be an upstream driver of MID1IP1 transcription. Furthermore, transcriptomic sequencing on MID1IP1-overexpressing HCC cells identified FOS-like 1 (FRA1) as a critical downstream mediator of MID1IP1. MID1IP1 upregulated FRA1 to subsequently promote its transcriptional activity and extracellular matrix degradation activity of matrix metalloproteinase MMP9, while knockdown of FRA1 effectively abolished the MID1IP1-induced migratory and invasive abilities. Conclusions: Our study identified MID1IP1 as a regulator in promoting FRA1-mediated-MMP9 signaling and demonstrated its role in HCC metastasis. Targeting MID1IP1-mediated FRA1 pathway may serve as a potential therapeutic strategy against HCC progression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Midline 1 interacting protein 1 promotes cancer metastasis through FOS-like 1-mediated matrix metalloproteinase 9 signaling in HCC

et al. 2023

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“…Post-translationally ACC is activated by the phosphorylation of several serine residues (Ser 79 , Ser 1200 , Ser 1215 ) by AMPK. ACC activity is also modulated by its ability to polymerize [ 181 ]. During insulin exposure, the proportion of active ACC polymers increases while catabolic hormones reduce ACC polymerization.…”

Section: Enzymes Of De Novo Lipogenesismentioning

confidence: 99%

Approaches to Measuring the Activity of Major Lipolytic and Lipogenic Enzymes In Vitro and Ex Vivo

Wilhelm

Rossmeislová

Šiklová

2022

IJMS

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Since the 1950s, one of the goals of adipose tissue research has been to determine lipolytic and lipogenic activity as the primary metabolic pathways affecting adipocyte health and size and thus representing potential therapeutic targets for the treatment of obesity and associated diseases. Nowadays, there is a relatively large number of methods to measure the activity of these pathways and involved enzymes, but their applicability to different biological samples is variable. Here, we review the characteristics of mean lipogenic and lipolytic enzymes, their inhibitors, and available methodologies for assessing their activity, and comment on the advantages and disadvantages of these methodologies and their applicability in vivo, ex vivo, and in vitro, i.e., in cells, organs and their respective extracts, with the emphasis on adipocytes and adipose tissue.

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Regulation of tumor metabolism by post translational modifications on metabolic enzymes

Dessai

Kalhotra²,

Novickis³

et al. 2022

Cancer Gene Ther

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MIG12 is involved in the LXR activation-mediated induction of the polymerization of mammalian acetyl-CoA carboxylase

Cited by 4 publications

References 22 publications

Midline 1 interacting protein 1 promotes cancer metastasis through FOS-like 1-mediated matrix metalloproteinase 9 signaling in HCC

Midline 1 interacting protein 1 promotes cancer metastasis through FOS-like 1-mediated matrix metalloproteinase 9 signaling in HCC

Approaches to Measuring the Activity of Major Lipolytic and Lipogenic Enzymes In Vitro and Ex Vivo

Regulation of tumor metabolism by post translational modifications on metabolic enzymes

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