Migration of microchimeric fetal cells into maternal circulation before placenta formation

Sunami, Rei; Komuro, Mayuko; Tagaya, Hikaru; Hirata, Shuji

doi:10.4161/chim.1.2.14301

Cited by 15 publications

(9 citation statements)

References 15 publications

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“…Though SRY gene is validated as an ideal sex-determining marker in the present study, there are few conditions in which SRY gene cannot be amplified, thereby leading to discrepancies between the genetic sex and phenotypical sex. These conditions are Turner syndrome (46,X0), Klinefelter syndrome (46,XXY), androgen insensitivity syndrome, Swyer syndrome caused by a mutation of SRY gene, 46,XX testicular disorder of sex development associated with the SRY gene, 47,XYY syndrome, 5-alpha-reductase deficiency, chimaerism and microchimaerism (Shahid et al 2010;Poplinski et al 2010;Audi et al 2010;Knower et al 2011;Wang et al 2004;Sunami et al 2010;Walker 2008;Bianchi et al 1996;Benito et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Isolation and quantification of DNA from epithelial cells obtained from acrylic removable partial denture for sex identification

Bharath

Saraswathi

Sajjan

et al. 2019

Egypt J Forensic Sci

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Background: This study is aimed to evaluate the use of epithelial cells adhering the acrylic removable partial denture in establishing the sex of the individual using sex-determining region of the Y chromosome (SRY) gene. With a hypothesis that prosthetic appliance can also be used as a source of DNA in forensics for sex determination. A sample of 30 partial dentures from different denture wearers was selected for this study. Informed consent was taken from the subjects and institutional ethical clearance was obtained for this study. Samples were then washed in saline and scrapings from 0.5 × 0.5 cm 2 of the tissue contacting surface were taken using a blade of fixed thickness. DNA extraction was done from these isolated epithelial cells using Real Genomics YGB 100 (Real Biotech Corporation, Taiwan). Quantification and purity assessment of the extracted DNA was done using a Nanodrop ND-1000 spectrophotometer and clean DNA was subjected to real-time PCR amplification using SRY sex-typing marker for the detection of the presence of the SRY gene. Results: Isolation of epithelial cells and extraction of DNA from all 30 acrylic removable partial dentures were possible. Average DNA yield quantified from all samples was 32.37 ng/μl with a minimum yield of 2.17 ng/μl and a maximum yield of 117.57 ng/μl. On amplification of the target sequence, sex determination was possible from all samples with 100% accuracy. Conclusion: Acrylic removable partial denture can also be used as a source of DNA in forensics for sex identification.

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Section: Discussionmentioning

confidence: 99%

Isolation and quantification of DNA from epithelial cells obtained from acrylic removable partial denture for sex identification

Bharath

Saraswathi

Sajjan

et al. 2019

Egypt J Forensic Sci

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“…Let us start with how microchimerism occurs. Although the precise timing of cell transfer remains to be clarified [8], microchimerism is establishedduring pregnancy [9,10]. A major question is how these maternal and fetal cells migratedfrom one body to the other.…”

Section: How Domicrochimeric Cells Pass Through the Placenta?mentioning

confidence: 99%

Emerging Questions in Materno-Fetal Microchimerism

Irie¹

2013

Reprod Sys Sexual Disorders

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Materno-fetal microchimerism is a common, lifelong chimeric state first established by the exchange ofsmall numbers of cells between the motherand the fetus during pregnancy. This apparently trivial phenomenon is now attracting attention due to its unexpected andprofound implications in the immune system. For example, is the placenta really an immunological barrier? How do we balance the internal environment despite the existence of an immunological non-self? In this review, I will discuss the pros and cons of materno-fetal microchimerismforour immune system (e.g., tolerance, materno-fetal immune disease, tissue regeneration, etc.) and the unanswered, puzzling aspects of microchimerismfrom theimmunological point of view.

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“…Le passage transplacentaire des cellules foetales débute très tôt au cours de la grossesse [32] et se prolonge pendant toute la grossesse pour être à son maximum dans les derniers mois de grossesse [33].…”

Section: Au Cours De La Grossesse Menée à Termeunclassified

Microchimérisme fœtal : soi et non soi, finalement qui sommes-nous ?

Boyon

Vinatier

2011

Journal de Gynécologie Obstétrique et Biologie de la Reproducti

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Migration of microchimeric fetal cells into maternal circulation before placenta formation

Cited by 15 publications

References 15 publications

Isolation and quantification of DNA from epithelial cells obtained from acrylic removable partial denture for sex identification

Isolation and quantification of DNA from epithelial cells obtained from acrylic removable partial denture for sex identification

Emerging Questions in Materno-Fetal Microchimerism

Microchimérisme fœtal : soi et non soi, finalement qui sommes-nous ?

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