Mild and severe cereal yellow dwarf viruses differ in silencing suppressor efficiency of the P0 protein

Almasi, Reza; Miller, W. Allen; Ziegler-Graff, Véronique

doi:10.1016/j.virusres.2015.06.020

Cited by 29 publications

(39 citation statements)

References 38 publications

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“…() and Almasi et al . (). However, the possibility remains that the ubiquitination process plays important roles in the AGO1 degradation triggered by P0 or infection of poleroviruses (Derrien et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Thus, it was proposed that the P0-SKP1 interaction is essential for RNA silencing suppression (Pazhouhandeh et al, 2006). However, several studies challenged the requirement of F-box-like motif or P0-SKP1 interaction for RNA silencing suppression (Zhuo et al, 2014;Almasi et al, 2015). A P0 encoded by PLRV-IM isolate is an VSR that suppresses RNA silencing by targeting AGO1 for degradation.…”

Section: Discussionmentioning

confidence: 99%

“…In recent several years, P0 proteins from various poleroviruses have been shown to function as VSRs (Pazhouhandeh et al, 2006;Mangwende et al, 2009;Csorba et al, 2010;Han et al, 2010;Kozlowska-Makulska et al, 2010;Delfosse et al, 2014;Zhuo et al, 2014;Almasi et al, 2015;Cascardo et al, 2015;Chen et al, 2016). Several regions within P0 are essential for their silencing suppression activity, including a consensus F-box-like motif (LPXX(L/I)X 10-13 P) and the Phe/Trp (FW) residues within the C-terminal consensus sequence ((K/R) IYGED GX 3 FWR) (Pazhouhandeh et al, 2006;Bortolamiol et al, 2007;Mangwende et al, 2009;Han et al, 2010;Fusaro et al, 2012;Delfosse et al, 2014;Zhuo et al, 2014;Almasi et al, 2015;Chen et al, 2016). P0 proteins encoded by TuYV and a few other polerovirus members were reported to interact with S phase kinase-associated protein 1 (SKP1), a member of the SKP1-Cullin 1-F-box (SCF) E3 ubiquitin ligase complex, through the consensus F-box-like motif (Pazhouhandeh et al, 2006;Zhuo et al, 2014;Almasi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Several regions within P0 are essential for their silencing suppression activity, including a consensus F-box-like motif (LPXX(L/I)X 10-13 P) and the Phe/Trp (FW) residues within the C-terminal consensus sequence ((K/R) IYGED GX 3 FWR) (Pazhouhandeh et al, 2006;Bortolamiol et al, 2007;Mangwende et al, 2009;Han et al, 2010;Fusaro et al, 2012;Delfosse et al, 2014;Zhuo et al, 2014;Almasi et al, 2015;Chen et al, 2016). P0 proteins encoded by TuYV and a few other polerovirus members were reported to interact with S phase kinase-associated protein 1 (SKP1), a member of the SKP1-Cullin 1-F-box (SCF) E3 ubiquitin ligase complex, through the consensus F-box-like motif (Pazhouhandeh et al, 2006;Zhuo et al, 2014;Almasi et al, 2015). As P0 was reported to interact with AGO1 in the nucleus and trigger ubiquitylation and degradation of AGO1 in plants (Baumberger et al, 2007;Bortolamiol et al, 2007;Csorba et al, 2010;Fusaro et al, 2012), it was initially considered to hijack the host SCF machinery to destabilize AGO1.…”

Section: Introductionmentioning

confidence: 99%

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Interaction between Brassica yellows virus silencing suppressor P0 and plant SKP1 facilitates stability of P0 in vivo against degradation by proteasome and autophagy pathways

Qi-zhong

Zhao

et al. 2019

New Phytologist

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Summary P0 protein of some polerovirus members can target ARGONAUTE 1 ( AGO 1) to suppress RNA silencing. Although P0 harbors an F‐box‐like motif reported to be essential for interaction with S phase kinase‐associated protein 1 ( SKP 1) and RNA silencing suppression, it is the autophagy pathway that was shown to contribute to AGO 1 degradation. Therefore, the role of P0– SKP 1 interaction in silencing suppression remains unclear. We conducted global mutagenesis and comparative functional analysis of P0 encoded by Brassica yellows virus (BrYV) (P0 Br ). We found that several residues within P0 Br are required for local and systemic silencing suppression activities. Remarkably, the F‐box‐like motif mutant of P0 Br , which failed to interact with SKP 1, is destabilized in vivo . Both the 26S proteasome system and autophagy pathway play a role in destabilization of the mutant protein. Furthermore, silencing of a Nicotiana benthamiana SKP 1 ortholog leads to the destabilization of P0 Br . Genetic analyses indicated that the P0 Br – SKP 1 interaction is not directly required for silencing suppression activity of P0 Br , but it facilitates stability of P0 Br to ensure efficient RNA silencing suppression. Consistent with these findings, efficient systemic infection of Br YV requires P0 Br . Our results reveal a novel strategy used by BrYV for facilitating viral suppressors of RNA silencing stability against degradation by plant cells.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Interaction between Brassica yellows virus silencing suppressor P0 and plant SKP1 facilitates stability of P0 in vivo against degradation by proteasome and autophagy pathways

Qi-zhong

Zhao

et al. 2019

New Phytologist

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“…BYDV-PAV's interactions with wheat and other annual cereals may have helped shape its genome. Unlike some other YDVs (Almasi, Miller & Ziegler-Graff 2015), BYDV-PAV lacks a P0 silencing suppressor (Miller & Rasochova 1997) for moderating plant defences and thus may face a tougher battle in well-defended perennials (Zhang, Lin & Xin 2009).…”

Section: Introductionmentioning

confidence: 99%

Crop‐associated virus infection in a native perennial grass: reduction in plant fitness and dynamic patterns of virus detection

et al. 2017

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Summary To understand the eco‐evolutionary significance of plant viruses in nature, we must (i) quantify the effects of infection on plant fitness and (ii) recognize that native plants are increasingly exposed to crop‐associated viruses. Studies of perennials are particularly needed: most of our knowledge of plant‐virus interactions is from annuals, yet long‐lived species dominate landscapes. Here we used aster models for life‐history analysis and longitudinal measures of plant virus status to evaluate multi‐year consequences of crop virus infection in a native perennial. We used Barley yellow dwarf virus acquired from wheat to inoculate seedlings of Panicum virgatum L. (switchgrass), a North American prairie grass. We grew inoculated and mock‐inoculated individuals of two ecotypes for 3 years in the field. We measured plant size, infection status and fitness components. Aster modelling provided integrated multi‐year measures of fitness. Crop virus inoculation reduced multi‐year native plant fitness by 30% over 2 years despite generally asymptomatic infection and evidence of resistance. This reduction was greater than predicted from individual fitness components or most size measures. Ecotypes differed in response, with the lowland ecotype experiencing higher apparent recovery from infection. Virus treatment in the upland ecotype delayed flowering phenology and reduced seed filling. Synthesis. Our use of field experimentation, surveys of plant infection status and aster modelling demonstrates a rigorous and broadly applicable approach for quantifying the effects of viruses and other microbes on multi‐year plant fitness. We found that a crop virus had negative multi‐year effects on native plant fitness even after infection was no longer detected. Viruses may have substantial effects on native vegetation with domestication of landscapes and agricultural expansion.

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Complete genome sequence of a novel wheat-infecting polerovirus associated with yellowing dwarf disease in China

Guo

Yuan

et al. 2022

Arch Virol

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Mild and severe cereal yellow dwarf viruses differ in silencing suppressor efficiency of the P0 protein

Cited by 29 publications

References 38 publications

Interaction between Brassica yellows virus silencing suppressor P0 and plant SKP1 facilitates stability of P0 in vivo against degradation by proteasome and autophagy pathways

Interaction between Brassica yellows virus silencing suppressor P0 and plant SKP1 facilitates stability of P0 in vivo against degradation by proteasome and autophagy pathways

Crop‐associated virus infection in a native perennial grass: reduction in plant fitness and dynamic patterns of virus detection

Complete genome sequence of a novel wheat-infecting polerovirus associated with yellowing dwarf disease in China

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