Milk supplemented with immune colostrum: Protection against rotavirus diarrhea and modulatory effect on the systemic and mucosal antibody responses in calves experimentally challenged with bovine rotavirus

Parreño, Viviana; Marcoppido, Gisela Ariana; Vega, Celina G.; Garaicoechea, Lorena; Rodriguez, D.; Saif, Linda J.; Férnandez, Francisco Tomás González

doi:10.1016/j.vetimm.2010.01.003

Cited by 37 publications

(53 citation statements)

References 26 publications

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“…However, milk antibodies drop rapidly in cattle and develops neonatal diarrhea often after calves become infected with rotavirus and coronavirus (Acres and Babiuk 1978). The development of vaccines or administration of milk supplements that make passive protection for the first several days of life induces high protection against bovine rotavirus and coronavirus (Parreñ o et al 2010). Previous studies have indicated that treatment with colostrum, monoclonal and polyclonal antibodies provided protection against bovine rotavirus and bovine coronavirus in calves and human (Fernandez et al 1998, Vega et al 2011.…”

Section: Discussionmentioning

confidence: 99%

Production and characterization of egg yolk antibody (IgY) against recombinant VP8-S2 antigen

Nasiri¹,

Nassiri²,

Tahmoorespur³

et al. 2016

Polish Journal of Veterinary Sciences

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Bovine Rotavirus and Bovine Coronavirus are the most important causes of diarrhea in newborn calves and in some other species such as pigs and sheep. VP8 subunit of rotavirus is the major determinant of the viral infectivity and neutralization. Spike glycoprotein of coronavirus is responsible for induction of neutralizing antibody response. Studies showed that immunoglobulin of egg yolk (IgY) from immunized hens has been identified to be a convenient source for specific antibodies for using in immunotherapy and immunodiagnostic to limit the infections.In this study, chimeric VP8-S2 gene was designed using by computational techniques. The chimeric VP8-S2 gene was cloned and sub-cloned into pGH and pET32a (+) vectors. Then, recombinant pET32a-VP8-S2 vector was transferred into E. coli BL21 CodonPlus (DE3). The expressed protein was purified by Ni-NTA chromatography column. Hens were immunized with the purified VP8-S2 protein three times. IgY was purified from egg yolks using polyethylene glycol precipitation method. Activity and specificity of anti-VP8-S2 IgY were detected by dot-blotting, Western-blotting and indirect ELISA.We obtained anti-VP8-S2 IgY by immunizing hens with the recombinant VP8-S2 protein. The anti-VP8-S2 IgY was showed to bind specifically to the chimeric VP8-S2 protein by dot-blotting, Western-blotting analyses and indirect ELISA. The result of this study indicated that such construction can be useful to investigate as candidates for development of detection methods for simultaneous diagnosis of both infections. Specific IgY against the recombinant VP8-S2 could be recommended as a candidate for passive immunization against bovine rotavirus and bovine coronavirus.

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Section: Discussionmentioning

confidence: 99%

Production and characterization of egg yolk antibody (IgY) against recombinant VP8-S2 antigen

Nasiri¹,

Nassiri²,

Tahmoorespur³

et al. 2016

Polish Journal of Veterinary Sciences

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“…Administration of colostrum preparations or milk replacers containing specific antibodies produced in hyperimmunized female animals has been shown to be beneficial; such products are now commercially available for use in calves (Parreno et al, 2010).…”

Section: Preventionmentioning

confidence: 99%

Rotavirus infections in domestic animals

Chatzopoulos

Athanasiou

Spyrou

et al. 2017

J Hellenic Vet Med Soc

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ABSTRACT. Rotaviruses are major enteric pathogens of humans and a wide variety of animals. Rotavirus infections have a worldwide prevalence. The viral genome is composed of 11 double-stranded RNA segments with six structural and five or six non-structural proteins. Over 35,000 strains have been identified and classified into five main (A, B, C, D, E) and three additional tentative (F, G, H) serotype groups. A binary classification system has been proposed defining 'G' or 'P' types, with at least 27 G and 35 P genotypes reported thusfar. The virus is transmitted primarily by the faecal-oral and oral routes. After attachment, entry into the host cells occurs through direct entry, fusion or endocytosis. Main mechanisms of Rotavirus-induced diarrhoea involve extensive enterocyte losses and nutrient disdigestion and malabsorption. Clinical features of Rotavirus infections range from asymptomatic infections to fulminant disease leading to rapid death. In calves, lambs and kids, piglets and foals, salient sign of the disease is diarrhoea; diarrhoeic faeces are white, yellow or, in severe cases, blood-tinted or frank haemorrhagic. In dogs and cats, the infection occurs usually as self-limiting diarrhoea. Avian Rotavirus infections are characterized by enteritis, growth depression and/or growth retardation. Definitive diagnosis of the infection can only be achieved by laboratory tests, including electron microscopic examination, immunohistochemical examination, immunofluorescence, ELISA, latex agglutination and molecular techniques. There is no specific treatment against Rotavirus infections. Treatment is based in providing supportive care and managing clinical signs and potential complications. Effective vaccines, containing inactivated, recombinant or attenuated strains of the virus, are available. Challenge studies have shown the ease of the virus in cross-infecting various animal species; animal strains of the virus may cross species and infect humans. Due to the ability of the virus to overcome species barriers, animal strains may act as natural source of viral genomes, promoting mutations and creating new viral genotypes, whose virulence cannot be predictable.

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“…31 The development of vaccines or administration of milk supplements will be cased passive protection for the first several days of life against BRV and BCV. 32 The development of epitope vaccines based on experimental research is very costly research which uses molecular biology and immunology technologies. The accuracy of this computational approach has been greatly enhanced using statistical methods.…”

Section: Protein Expression and Purification Analysesmentioning

confidence: 99%

Design and Construction of Chimeric VP8-S2 Antigen for Bovine Rotavirus and Bovine Coronavirus

Nasiri¹,

Nassiri²,

Tahmoorespur³

et al. 2016

Adv Pharm Bull

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Milk supplemented with immune colostrum: Protection against rotavirus diarrhea and modulatory effect on the systemic and mucosal antibody responses in calves experimentally challenged with bovine rotavirus

Cited by 37 publications

References 26 publications

Production and characterization of egg yolk antibody (IgY) against recombinant VP8-S2 antigen

Production and characterization of egg yolk antibody (IgY) against recombinant VP8-S2 antigen

Rotavirus infections in domestic animals

Design and Construction of Chimeric VP8-S2 Antigen for Bovine Rotavirus and Bovine Coronavirus

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