Mimicking the functional hematopoietic stem cell niche in vitro: recapitulation of marrow physiology by hydrogel-based three-dimensional cultures of mesenchymal stromal cells

Sharma, Monika B.; Limaye, Lalita; Kale, Vaijayanti

doi:10.3324/haematol.2011.050500

Cited by 112 publications

(121 citation statements)

References 62 publications

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“…This is in agreement with other 3D culture approaches [32,38,51], which collectively demonstrate that levels of key endosteal factors, such as FN, VN, OPN and ALP, are enhanced in 3D compared to 2D controls.…”

Section: Discussionsupporting

confidence: 78%

“…MSCs are sourced from multiple tissues, including bone marrow [49] and placenta [50]. Bone marrow-derived MSCs (bmMSCs) have been used to develop in vitro models of the HSC niche [32,38,51]. However, plMSCs are more accessible than bmMSCs in terms of their isolation procedures and cell numbers per donor [52].…”

Section: Discussionmentioning

confidence: 99%

“…Tissue-engineered approaches to the HSC niche are frequently based on hydrogels [32,[37][38][39][40]. However, the use of solid scaffolds with higher mechanical properties allows more complex approaches.…”

Section: Discussionmentioning

confidence: 99%

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

et al. 2017

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds, Acta Biomaterialia (2016), doi: http://dx.doi.org/10. 1016/j.actbio.2016.12.040 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. models that mimic the endosteal microenvironment enable researchers to discover the causes and improve treatments for blood and immune-related diseases. The aim of this study was to establish a physiologically relevant in vitro model using 3D printed scaffolds to assess the contribution of human cells to the formation of a construct that mimics human endosteum. Melt electrospun written scaffolds were used to compare the suitability of primary human osteoblasts (hOBs) and placenta-derived mesenchymal stem cells (plMSCs) in ( when compared to HSCs maintained using tissue culture plastic. This 3D testing platform represents an endosteal bone-like platform and warrants future investigation for the maintenance and expansion of human HSCs.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

et al. 2017

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“…Most of this attachment will be mediated by the ECM binding integrins present on the cell surface. The ECM motifs are either provided directly by the material used (gelatin, collagen, fibronectin), by serum adhesion proteins (Kobayashi et al, 2005;Dodo et al, 2013), or early deposition of ECM by the cells themselves (Pearlstein et al, 1980;Desai et EM Schutgens et al Biomaterials for intervertebral disc regeneration al., 2014;Jin et al, 2007;Sharma et al, 2012;Xu et al, 2013). Integrin-mediated cell-matrix binding not only affects chondrogenic cell survival and proliferation in vitro and in vivo (Gilchrist et al, 2007;Loeser, 2014), but is also involved in cell differentiation by regulating the response to materials with different mechanical properties.…”

Section: Extracellular Matrix-cell Interactionmentioning

confidence: 99%

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

Schutgens¹,

Tryfonidou²,

Smit³

et al. 2015

eCM

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Intervertebral disc (IVD) degeneration is associated with most cases of cervical and lumbar spine pathologies, amongst which chronic low back pain has become the number one cause of loss of quality-adjusted life years. In search of alternatives to the current less than optimal and usually highly invasive treatments, regenerative strategies are being devised, none of which has reached clinical practice as yet. Strategies include the use of stem cells, gene therapy, growth factors and biomaterial carriers. Biomaterial carriers are an important component in musculoskeletal regenerative medicine techniques. Several biomaterials, both from natural and synthetic origin, have been used for regeneration of the IVD in vitro and in vivo. Aspects such as ease of use, mechanical properties, regenerative capacity, and their applicability as carriers for regenerative and anti-degenerative factors determine their suitability for IVD regeneration. The current review provides an overview of the biomaterials used with respect to these properties, including their drawbacks. In addition, as biomaterial application until now appears to have been based on a mix of mere availability and intuition, a more rational design is proposed for future use of biomaterials for IVD regeneration. Ideally, high-throughput screening is used to identify optimally effective materials, or alternatively medium content comparative studies should be carried out to determine an appropriate reference material for future studies on novel materials.

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“…It was previously shown that arterioles are associated with both quiescent NG2 + niche cells and HSCs, suggesting that the vessel itself may be a critical gatekeeper of stem cell quiescence in the BM (Kunisaki et al, 2013). A culture system that closely recapitulates marrow physiology is essential to study the niche-mediated regulation of HSC fate (Sharma et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Expansion of human umbilical cord blood hematopoieticprogenitors with cord vein pericytes

Saltik¹,

Yağcı²

2017

Turk J Biol

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The vascular niche is a site rich in blood vessels, whereas endothelial cells, pericytes, and smooth muscle cells create a microenvironment that recruits mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs), which is important for stem cell mobilization, proliferation, and differentiation. In this study, CD146 + pericytes were purified and enriched from the human umbilical cord vein. In order to define their direct role in hematopoiesis, we tested the CD146 + pericytes as compared with osteoblasts derived from umbilical cord blood (UCB) MSCs to sustain human UCB hematopoietic progenitor cells in noncontact coculture settings or in culture media previously conditioned (CM) by these cells. The growth of UCB cells was the greatest in pericyte cocultures (2.8-fold vs. the control). The increased growth in pericyte and pericyte CM cultures was largely the result of increased frequency of CD34 + and CD38 + hematopoietic progenitors, CD34 + CD41 + megakaryocyte progenitors, and CD235 + erythroblasts. A total of 29 factors were found to be secreted by pericytes higher than by osteoblasts. The most secreted growth factor by pericytes was vascular endothelial growth factor (1.3-fold). We demonstrate for the first time that human CD146 + perivascular cell coculture and CM are able to directly support the ex vivo maintenance of human hematopoietic progenitor cells.

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Mimicking the functional hematopoietic stem cell niche in vitro: recapitulation of marrow physiology by hydrogel-based three-dimensional cultures of mesenchymal stromal cells

Cited by 112 publications

References 62 publications

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

Expansion of human umbilical cord blood hematopoieticprogenitors with cord vein pericytes

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