Minc03328 effector gene downregulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana

Moreira, Valdeir Junio Vaz; Lourenço-Tessutti, Isabela Tristan; Basso, Marcos Fernando; Lisei-de-Sá, Maria Eugênia; Morgante, Carolina Vianna; Paes-de-Melo, Bruno; Arraes, Fabrício Barbosa Monteiro; Martins-de-Sa, Diogo; Silva, Maria Cristina Mattar; Engler, Janice de Almeida; Grossi-de-Sá, Maria Fátima

doi:10.1007/s00425-022-03823-4

Cited by 11 publications

(5 citation statements)

References 52 publications

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“…This immune response is often, but not exclusively, visualized by the hypersensitivity response (HR), which is characterized by a rapid cell death at the point of pathogen entry and reduction of the nematode’s feeding site [ 26 ]. Several nematode effectors [ 27 , 28 , 29 , 30 , 31 , 32 , 33 ] and plant R genes [ 34 , 35 , 36 , 37 , 38 , 39 ] have been characterized. Studies have even showed evidence in soybean the participation of non-canonical R genes in resistance to M. incognita [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

Integrated Omic Approaches Reveal Molecular Mechanisms of Tolerance during Soybean and Meloidogyne incognita Interactions

Arraes

Vasquez

Tahir

et al. 2022

Plants

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The root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI 595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematode infestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT–qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10 overexpression can be effective against the plant parasitic nematode M. incognita, but its mechanism of action remains unclear. These findings will help develop new engineered soybean genotypes with higher performance in response to RKN infections.

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Section: Introductionmentioning

confidence: 99%

Integrated Omic Approaches Reveal Molecular Mechanisms of Tolerance during Soybean and Meloidogyne incognita Interactions

Arraes

Vasquez

Tahir

et al. 2022

Plants

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“…Moreover, we found that the nematode secretes NEROSs in the giant cells, concentrated in giant cell foci clearly visible at later parasitic stages. While other nematode effectors have been localized, most of them target the nuclei, cytosol, or the apoplast (Naalden et al ., 2018; Zhao et al ., 2020; Moreira et al ., 2022) or they were shown to be in leaf chloroplasts, but not in infected roots (Lin et al ., 2015). Hence, this is the first study where such localization of giant cell foci has been reported.…”

Section: Discussionmentioning

confidence: 99%

The nematode effector Mj‐NEROSs interacts with Rieske's iron–sulfur protein influencing plastid ROS production to suppress plant immunity

Stojilković,

Xiang,

Chen

et al. 2024

New Phytologist

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Summary Root‐knot nematodes (RKN; Meloidogyne species) are plant pathogens that introduce several effectors in their hosts to facilitate infection. The actual targets and functioning mechanism of these effectors largely remain unexplored. This study illuminates the role and interplay of the Meloidogyne javanica nematode effector ROS suppressor (Mj‐NEROSs) within the host plant environment. Mj‐NEROSs suppresses INF1‐induced cell death as well as flg22‐induced callose deposition and reactive oxygen species (ROS) production. A transcriptome analysis highlighted the downregulation of ROS‐related genes upon Mj‐NEROSs expression. NEROSs interacts with the plant Rieske's iron–sulfur protein (ISP) as shown by yeast‐two‐hybrid and bimolecular fluorescence complementation. Secreted from the subventral pharyngeal glands into giant cells, Mj‐NEROSs localizes in the plastids where it interacts with ISP, subsequently altering electron transport rates and ROS production. Moreover, our results demonstrate that isp Arabidopsis thaliana mutants exhibit increased susceptibility to M. javanica, indicating ISP importance for plant immunity. The interaction of a nematode effector with a plastid protein highlights the possible role of root plastids in plant defense, prompting many questions on the details of this process.

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“…1b ). While other nematode effectors have been localized, most of them target the nuclei, cytosol, or the apoplast 13,59,60 . Hence, this is the first study where such localization of giant cell foci has been reported.…”

Section: Discussionmentioning

confidence: 99%

The nematode effector Mj-NEROSs interacts with ISP influencing plastid ROS production to suppress plant immunity

Stojilković

Xiang

Chen

et al. 2022

Preprint

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Root-knot nematodes are an important group of plant pathogens that mainly infect plant roots. They establish a feeding site in the host upon infection while secreting hundreds of effectors. These effector proteins are crucial for successful pathogen propagation. Although many effectors have been described, their targets and molecular mode of action are still unknown. Here we report the analysis of the RKN effector, Mj-NEROSs (Meloidogyne javanica nematode effector ROS suppressor), which emerges to have an essential role in suppressing host immunity by inhibiting INF1-induced cell death and reducing callose deposition. Secreted from the subventral esophageal glands to giant cells, Mj-NEROSs localizes in plastids where it interacts with ISP, interfering with the electron transport rate and ROS production. Moreover, our transcriptome analysis shows the downregulation of ROS-related genes upon Mj-NEROSs expression. We propose that Mj-NEROSs manipulates root plastids leading to transcriptional changes, lowering ROS production, and suppressing host immunity.

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Minc03328 effector gene downregulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana

Cited by 11 publications

References 52 publications

Integrated Omic Approaches Reveal Molecular Mechanisms of Tolerance during Soybean and Meloidogyne incognita Interactions

Integrated Omic Approaches Reveal Molecular Mechanisms of Tolerance during Soybean and Meloidogyne incognita Interactions

The nematode effector Mj‐NEROSs interacts with Rieske's iron–sulfur protein influencing plastid ROS production to suppress plant immunity

The nematode effector Mj-NEROSs interacts with ISP influencing plastid ROS production to suppress plant immunity

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