Mind your media

Lagziel, Shoval; Gottlieb, Eyal; Shlomi, Tomer

doi:10.1038/s42255-020-00299-y

Cited by 44 publications

(33 citation statements)

References 20 publications

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“…Alongside model selection, experimental conditions and media selection are other areas that are gaining awareness, including physiologic media that more closely resembles human or rodent plasma compared to traditional cell culture media [323]. While the current focus of these physiological media rightly focused on amino acid and glucose levels [324], there remains a significant challenge to have these media reflect the physiological lipid environment. This issue already exists with existing cell culture approaches, which was comprehensively reported recently by Prof Else, where he concluded that "Fetal bovine serum… at 10% of media provides 2-3% of the fatty acid and cholesterol, 1% of the PUFA and 0.3% of the essential fatty acid linoleic acid (18: 2n-6) available to cells in the body" [325].…”

Section: Aspects Of Tumor Fatty Acid Metabolism That Warrant Closer Amentioning

confidence: 99%

The diversity and breadth of cancer cell fatty acid metabolism

2021

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Tumor cellular metabolism exhibits distinguishing features that collectively enhance biomass synthesis while maintaining redox balance and cellular homeostasis. These attributes reflect the complex interactions between cell-intrinsic factors such as genomic-transcriptomic regulation and cell-extrinsic influences, including growth factor and nutrient availability. Alongside glucose and amino acid metabolism, fatty acid metabolism supports tumorigenesis and disease progression through a range of processes including membrane biosynthesis, energy storage and production, and generation of signaling intermediates. Here, we highlight the complexity of cellular fatty acid metabolism in cancer, the various inputs and outputs of the intracellular free fatty acid pool, and the numerous ways that these pathways influence disease behavior.

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Section: Aspects Of Tumor Fatty Acid Metabolism That Warrant Closer Amentioning

confidence: 99%

The diversity and breadth of cancer cell fatty acid metabolism

2021

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“…In addition, the metabolic attributes of tissue resident parenchymal cells have the potential to shape the metabolic responses of both infiltrating leukocytes and bacteria, revealing another level of complexity. Considerable care must be taken when designing experiments to deconstruct these complex systems in vitro, since the composition of mammalian cell culture media can have drastic deviations from metabolite concentrations found in human plasma (167). Therefore, findings must be validated in leukocytes and bacteria immediately ex vivo, as the selection of in vitro culture conditions could result in metabolic changes that are not reflective of in vivo infection (167).…”

Section: Discussionmentioning

confidence: 99%

Crosstalk Between Staphylococcus aureus and Innate Immunity: Focus on Immunometabolism

Horn

Kielian

2021

Front. Immunol.

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Staphylococcus aureus is a leading cause of bacterial infections globally in both healthcare and community settings. The success of this bacterium is the product of an expansive repertoire of virulence factors in combination with acquired antibiotic resistance and propensity for biofilm formation. S. aureus leverages these factors to adapt to and subvert the host immune response. With the burgeoning field of immunometabolism, it has become clear that the metabolic program of leukocytes dictates their inflammatory status and overall effectiveness in clearing an infection. The metabolic flexibility of S. aureus offers an inherent means by which the pathogen could manipulate the infection milieu to promote its survival. The exact metabolic pathways that S. aureus influences in leukocytes are not entirely understood, and more work is needed to understand how S. aureus co-opts leukocyte metabolism to gain an advantage. In this review, we discuss the current knowledge concerning how metabolic biases dictate the pro- vs. anti-inflammatory attributes of various innate immune populations, how S. aureus metabolism influences leukocyte activation, and compare this with other bacterial pathogens. A better understanding of the metabolic crosstalk between S. aureus and leukocytes may unveil novel therapeutic strategies to combat these devastating infections.

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“…Because nutrient media can shape the physiology of cultured cells ( 42 ), the use of so-called “physiological media” (i.e., media that better reflect physiological conditions) have seen a recent spike in interest ( 43 ). Similarly, as observed with Salmonella enterica , medium composition (i.e., high concentration of glucose) can influence pathogen intracellular replication and mask the metabolic state of the bacterium during infection ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolic Plasticity Aids Amphotropism of Coxiella burnetii

2021

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Coxiella burnetii , the causative agent of Query (Q) fever in humans, is an obligate intracellular bacterium. C. burnetii can naturally infect a broad range of host organisms (e.g., mammals and arthropods) and cell types. This amphotropic nature of C. burnetii , in combination with its ability to utilize both glycolytic and gluconeogenic carbon sources, suggests that the pathogen relies on metabolic plasticity to replicate in nutritionally diverse intracellular environments. To test the significance of metabolic plasticity in C. burnetii host cell colonization, C. burnetii intracellular replication in seven distinct cell lines was compared between a metabolically competent parental strain and a mutant, Cb Δ pckA, unable to undergo gluconeogenesis. Both the parental strain and Cb Δ pckA exhibited host cell-dependent infection phenotypes, which were influenced by alterations to host glycolytic or gluconeogenic substrate availability. Because the nutritional environment directly impacts host cell physiology, our analysis was extended to investigate the response of C. burnetii replication in mammalian host cells cultivated in a novel physiological medium based on the nutrient composition of mammalian interstitial fluid, Interstitial Fluid-modeled Medium (IFmM). An infection model based on IFmM resulted in exacerbation of a replication defect exhibited by Cb Δ pckA in specific cell lines. Cb Δ pckA was also attenuated during infection of an animal host. Overall, the study underscores that gluconeogenic capacity aids C. burnetii amphotropism and that the amphotropic nature of C. burnetii should be considered when resolving virulence mechanisms in this pathogen.

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Mind your media

Cited by 44 publications

References 20 publications

The diversity and breadth of cancer cell fatty acid metabolism

The diversity and breadth of cancer cell fatty acid metabolism

Crosstalk Between Staphylococcus aureus and Innate Immunity: Focus on Immunometabolism

Metabolic Plasticity Aids Amphotropism of Coxiella burnetii

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