Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp.

Abstract

A bacterial strain (Serratia sp.) that could transform chlorpyrifos to 3,5,6-trichloro-2-pyridinol (TCP) and a TCP-mineralizing fungal strain (Trichosporon sp.) were isolated from activated sludge by enrichment culture technique. The fungus could also degrade 50 mg chlorpyrifos l(-1) within 7 days. Co-cultures completely mineralized 50 mg chlorpyrifos l(-1) within 18 h at 30 degrees C and pH 8 using a total inocula of 0.15 g biomass l(-1).

Determination of selected pesticides in water samples adjacent to agricultural fields and removal of organophosphorus insecticide chlorpyrifos using soil bacterial isolates

“…But the bacterial growth and chlorpyrifos degradation were both increased rapidly during 2 days of incubation in chlorpyrifos-containing medium. Result also represented that, when growth of the bacterial cell increased, chlorpyrifos degradation also relatively increased, similar results also found in other studies (Xu et al 2007(Xu et al , 2008. Xu et al (2007) observed the complete disappearance of 50 mg/l chlorpyrifos due to Serratia sp.…”

Novel Gene Clusters and Metabolic Pathway Involved in 3,5,6-Trichloro-2-Pyridinol Degradation by Ralstonia sp. Strain T6

“…strain 7 (11), Trichosporon sp. strain TCF (12), Bacillus pumilus C2A1 (13), Pseudomonas strain M285 (14), and Ralstonia sp. strain T6 (15).…”

Development of an Autofluorescent Whole-Cell Biocatalyst by Displaying Dual Functional Moieties on Escherichia coli Cell Surfaces and Construction of a Coculture with Organophosphate-Mineralizing Activity

“…Complete mineralization of parathion or chlorpyrifos has been achieved by coculture of two genetically engineered strains (13) or two natural degraders (46), respectively. In this study, a coculture comprised of two metabolically complementary bacteria, E. coli XL1-Blue with OPH activity and Ochrobactrum sp.…”

“…One strategy to avoid the generation of toxic hydrolytic products is to combine bacteria with complementary metabolic pathways into functional assemblages. This approach has been used for biodegradation of several xenobiotic pollutants, including 4-chlorodibenzofuran (1), parathion (13), and chlorpyrifos (46).…”