Mingmu Xiaoyao granules regulate the PI3K/Akt/mTOR signaling pathway to reduce anxiety and depression and reverse retinal abnormalities in rats

Ma, Qiuyan; Zhou, Jian; Yang, Ziyi; Xie, Xinran; Li, Tiejun; Yang, Youwen

doi:10.3389/fphar.2022.1003614

Cited by 9 publications

(3 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Collectively, these cytokines can affect the PI3K-Akt, Ras, MAPK, and Jak–STAT signaling pathways ( Figure 2B ). The role of PI3K-Akt ( Jacot and Sherris, 2011 ; Ma et al, 2022 ), Ras ( Wang et al, 2015 ), MAPK ( Supanji et al, 2013 ; Moustardas et al, 2023 ), and Jak–STAT ( Chen et al, 2016 , 2019 ; Hombrebueno et al, 2020 ; Cho et al, 2022 ) pathways in inflammatory and degeneration retinal diseases such as diabetic retinopathy and age-related macular degeneration has been well appreciated. Our results suggest that they may also be involved in RVO-mediated retinopathy.…”

Section: Discussionmentioning

confidence: 99%

Increased intraocular inflammation in retinal vein occlusion is independent of circulating immune mediators and is involved in retinal oedema

Zhou

Liu

et al. 2023

Front. Neurosci.

View full text Add to dashboard Cite

We aim to understand the link between systemic and intraocular levels of inflammatory mediators in treatment-naïve retinal vein occlusion (RVO) patients, and the relationship between inflammatory mediators and retinal pathologies. Twenty inflammatory mediators were measured in this study, including IL-17E, Flt-3 L, IL-3, IL-8, IL-33, MIP-3β, MIP-1α, GRO β, PD-L1, CD40L, IFN-β, G-CSF, Granzyme B, TRAIL, EGF, PDGF-AA, PDGF-AB/BB, TGF-α, VEGF, and FGFβ. RVO patients had significantly higher levels of Flt-3 L, IL-8, MIP-3β, GROβ, and VEGF, but lower levels of EGF in the aqueous humor than cataract controls. The levels of Flt-3 L, IL-3, IL-33, MIP-1α, PD-L1, CD40 L, G-CSF, TRAIL, PDGF-AB/BB, TGF-α, and VEGF were significantly higher in CRVO than in BRVO. KEGG pathway enrichment revealed that these mediators affected the PI3K-Akt, Ras, MAPK, and Jak/STAT signaling pathways. Protein–Protein Interaction (PPI) analysis showed that VEGF is the upstream cytokine that influences IL-8, G-CSF, and IL-33 in RVO. In the plasma, the level of GROβ was lower in RVO than in controls and no alterations were observed in other mediators. Retinal thickness [including central retinal thickness (CRT) and inner limiting membrane to inner plexiform layer (ILM-IPL)] positively correlated with the intraocular levels of Flt-3 L, IL-33, GROβ, PD-L1, G-CSF, and TGF-α. The size of the foveal avascular zone positively correlated with systemic factors, including the plasma levels of IL-17E, IL-33, INF-β, GROβ, Granzyme B, and FGFβ and circulating high/low-density lipids and total cholesterols. Our results suggest that intraocular inflammation in RVO is driven primarily by local factors but not circulating immune mediators. Intraocular inflammation may promote macular oedema through the PI3K-Akt, Ras, MAPK, and Jak/STAT signaling pathways in RVO. Systemic factors, including cytokines and lipid levels may be involved in retinal microvascular remodeling.

show abstract

Section: Discussionmentioning

confidence: 99%

Increased intraocular inflammation in retinal vein occlusion is independent of circulating immune mediators and is involved in retinal oedema

Zhou

Liu

et al. 2023

Front. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Another study discovered that modified XYS inhibits M1 polarization of microglia and alleviates neuroinflammation by activating the PI3K/AKT/mTOR pathway to induce autophagy (Su et al, 2023). The MingmuXiaoyao granule, a modified compound derived from XYS, has been observed to regulate autophagy through modulation of the PI3K/AKT/mTOR signaling pathway, thereby enhancing retinal morphology and function, as well as alleviating depression-like behavior in rats subjected to CUMS (Ma et al, 2022).…”

Section: Tcm Compoundsmentioning

confidence: 99%

Antidepressant pharmacological mechanisms: focusing on the regulation of autophagy

Lv,

Zhang,

Huang

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

The core symptoms of depression are anhedonia and persistent hopelessness. Selective serotonin reuptake inhibitors (SSRIs) and their related medications are commonly used for clinical treatment, despite their significant adverse effects. Traditional Chinese medicine with its multiple targets, channels, and compounds, exhibit immense potential in treating depression. Autophagy, a vital process in depression pathology, has emerged as a promising target for intervention. This review summarized the pharmacological mechanisms of antidepressants by regulating autophagy. We presented insights from recent studies, discussed current research limitations, and proposed new strategies for basic research and their clinical application in depression.

show abstract

“…In the process of sample preparation, we combined 1000 µL of Shenfu injection solution with 10 µL of an internal standard, followed by vortexing for 30 seconds. The concrete procedures for centrifugation, ltration, analysis, and step-wise gradient elution can be found in reference (37). Metabolite identi cation and structural analysis relied on the BIOTREE Traditional Chinese Medicine database from BIOTREE Biotechnology Co., Ltd.…”

Section: Uhplc-qe-ms Data Processing and Statistical Analysismentioning

confidence: 99%

Shenfu Injection Regulates Pyroptosis through the NLRP3/Caspase-1 Pathway to Improve Chronic Heart Failure

FAN,

LIAO,

HUANG

et al. 2023

Preprint

View full text Add to dashboard Cite

Objective: The objective of this research is to clarify how Shenfu injection enhances chronic heart failure treatment, specifically by examining its influence on the NLRP3-Caspase-1 pathway. Methods: The chronic heart failure in rats was induced through subcutaneous administration of isoproterenol. The effectiveness of Shenfu injection was assessed by examining cardiac function using echocardiography, monitoring changes in serum N-terminal brain natriuretic peptide levels, and conducting histological analysis with HE and Masson staining to evaluate modifications in myocardial cell structure and fibrosis. To assess the protein levels associated with the NLRP3/Caspase-1 pathway, cellular pyroptosis, and myocardial ultrastructure, various techniques including Western blotting, GSDMD-N staining, TUNEL immunofluorescence dual staining, and electron microscopy were employed. Results: Shenfu injection significantly ameliorated cardiac function, myocardial arrangement, and fibrosis in chronic heart failure rats. Significantly, it substantially decreased the protein levels of NLRP3, caspase-1, IL-1β, and IL-18, along with a reduction in cellular pyroptosis positivity (P < 0.05). Under electron microscopy, the morphology appeared normal, with only partial cell membranes exhibiting pore formation. Conclusion: In summary, our research indicates that the SFI intervention improves cardiac function in the chronic heart failure model induced by isoproterenol, possibly by regulating the NLRP3/Caspase-1 pathway to reduce pyroptosis, suppress inflammatory responses, protect myocardial cells, and maintain the integrity of cellular ultrastructure.

show abstract

Mingmu Xiaoyao granules regulate the PI3K/Akt/mTOR signaling pathway to reduce anxiety and depression and reverse retinal abnormalities in rats

Cited by 9 publications

References 83 publications

Increased intraocular inflammation in retinal vein occlusion is independent of circulating immune mediators and is involved in retinal oedema

Increased intraocular inflammation in retinal vein occlusion is independent of circulating immune mediators and is involved in retinal oedema

Antidepressant pharmacological mechanisms: focusing on the regulation of autophagy

Shenfu Injection Regulates Pyroptosis through the NLRP3/Caspase-1 Pathway to Improve Chronic Heart Failure

Contact Info

Product

Resources

About