Mini Review: The Forensic Value of Heat Shock Proteins

Prangenberg, Julian; Doberentz, Elke; Mawick, Anthea; Madea, Burkhard

doi:10.3389/fmed.2021.800100

Cited by 3 publications

(6 citation statements)

References 29 publications

(55 reference statements)

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“…Additionally, HSP60 regulates mitochondrial permeability transition, establishing a cytoprotective network that counters CypD-associated cell death in tumor contexts, where CypD is a component of the mitochondrial permeability transition pore. Furthermore, HSP60 plays a crucial role in protein import and quality control machinery[ 68 , 69 ].…”

Section: Discussionmentioning

confidence: 99%

Prognosis value of heat-shock proteins in esophageal and esophagogastric cancer: A systematic review and meta-analysis

Nakamura,

Park,

Pereira

et al. 2024

World J Gastrointest Oncol

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BACKGROUND Heat shock proteins (HSPs) are molecular chaperones that play an important role in cellular protection against stress events and have been reported to be overexpressed in many cancers. The prognostic significance of HSPs and their regulatory factors, such as heat shock factor 1 (HSF1) and CHIP, are poorly understood. AIM To investigate the relationship between HSP expression and prognosis in esophageal and esophagogastric cancer. METHODS A systematic review was conducted in accordance with PRISMA recommendations (PROSPERO: CRD42022370653), on Embase, PubMed, Cochrane, and LILACS. Cohort, case-control, and cross-sectional studies of patients with esophagus or esophagogastric cancer were included. HSP-positive patients were compared with HSP-negative, and the endpoints analyzed were lymph node metastasis, tumor depth, distant metastasis, and overall survival (OS). HSPs were stratified according to the HSP family, and the summary risk difference (RD) was calculated using a random-effect model. RESULTS The final selection comprised 27 studies, including esophageal squamous cell carcinoma (21), esophagogastric adenocarcinoma (5), and mixed neoplasms (1). The pooled sample size was 3465 patients. HSP40 and 60 were associated with a higher 3-year OS [HSP40: RD = 0.22; 95% confidence interval (CI): 0.09-0.35; HSP60: RD = 0.33; 95%CI: 0.17-0.50], while HSF1 was associated with a poor 3-year OS (RD = -0.22; 95%CI: -0.32 to -0.12). The other HSP families were not associated with long-term survival. HSF1 was associated with a higher probability of lymph node metastasis (RD = -0.16; 95%CI: -0.29 to -0.04). HSP40 was associated with a lower probability of lymph node dissemination (RD = 0.18; 95%CI: 0.03-0.33). The expression of other HSP families was not significantly related to tumor depth and lymph node or distant metastasis. CONCLUSION The expression levels of certain families of HSP, such as HSP40 and 60 and HSF1, are associated with long-term survival and lymph node dissemination in patients with esophageal and esophagogastric cancer.

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Section: Discussionmentioning

confidence: 99%

Prognosis value of heat-shock proteins in esophageal and esophagogastric cancer: A systematic review and meta-analysis

Nakamura,

Park,

Pereira

et al. 2024

World J Gastrointest Oncol

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“…It is an essential work to determine wound vitality and wound age in forensic autopsy cases. To achieve the purpose, advanced biological techniques are applied to forensic pathology ( 4 – 8 ). In forensic medicine, the compression mark on the neck is one of the most important criteria for determining whether the neck has been affected by the contractile force.…”

Section: Discussionmentioning

confidence: 99%

“…Prangenberg et al ( 4 ) showed heat shock protein was widely applied to forensic pathology such as fire-related death, hypothermia, cardiac death, drowning, excited delirium, trauma and SIDS. Ubiquitin is known as a heat-shock protein induced by several stress conditions ( 18 ).…”

Section: Discussionmentioning

confidence: 99%

“…In forensic practices, differentiating antemortem injury from postmortem damage is one of the important issues, which is a classical but still modern topic ( 1 – 3 ). Thus, there are numerous forensic studies exploring available markers for the determination of wound vitality using biochemical, histochemical, immunohistochemical and molecular biological techniques ( 4 – 6 ). Moreover, there are several recent studies focusing on omics sciences and micro RNA ( 7 , 8 ).…”

Section: Introductionmentioning

confidence: 99%

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Forensic Application of Epidermal Ubiquitin Expression to Determination of Wound Vitality in Human Compressed Neck Skin

et al. 2022

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Ubiquitin is a member of the heat shock protein family and is rapidly induced by various types of stimuli, including ischemic and mechanical stress. However, its significance in determining wound vitality of neck compression skin in forensic pathology remains unclear. We immunohistochemically examined the expression of ubiquitin in the neck skin samples to understand its forensic applicability in determining wound vitality. Skin samples were obtained from 53 cases of neck compression (hanging, 42 cases; strangulation, 11 cases) during forensic autopsies. Intact skin from the same individual was used as the control. Ubiquitin expression was detected in 73.9% of keratinocytes in intact skin samples, but only in 21.2% of keratinocytes in the compression regions, with statistical differences between the control and compression groups. This depletion in the case of neck compression may be caused by the impaired conversion of conjugated to free ubiquitin and failure of de novo ubiquitin synthesis. From a forensic pathological perspective, immunohistochemical examination of ubiquitin expression in the skin of the neck can be regarded as a valuable marker for diagnosing traces of antemortem compression.

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“…The HSP expression under heat stress has already been demonstrated in myocardium, lung and kidney tissues and HSPs can be visualized with immunohistochemical staining 22 – 24 . Not only fire deaths but also cardiac deaths, mechanical asphyxiation and acute lung injury, HSPs were detected 24 . In the present study, we investigated the expression of HSPs under hypoxic conditions of blood flow stasis.…”

Section: Introductionmentioning

confidence: 99%

Relationship between intrathrombotic appearance of HSP27 and HSP70 and thrombus ages in a murine model of deep vein thrombosis

Nosaka,

Ishida,

Kuninaka

et al. 2023

Sci Rep

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Heat shock proteins (HSPs) are molecular chaperones whose primary function is cytoprotection, supporting cell survival under (sub) lethal conditions. They have been implicated in various diseases such as inflammatory diseases and cancer due to their cytoprotective and immunomodulatory effects, and their biological mechanisms have been studied. Central family members include, HSP27, which is induced by various stimuli such as heat shock, hypoxia, hyperoxia, ultraviolet exposure, and nutritional deficiency, and HSP70, which is homeostatically expressed in many organs such as the gastrointestinal tract and has anti-cell death and anti-inflammatory effects. In this study, HSP27 and HSP70 were investigated during thrombus formation and dissolution in a deep vein thrombosis model by immunohistochemistry to determine their involvement in this process and whether their expression could be used as a forensic marker. In the process of thrombus formation and lysis, HSP27 and HSP70 were found to be expressed by immunohistochemical analysis. The role of inhibitors of HSP27 and HSP70 in the pathogenesis of thrombosis in mice was also investigated. When HSP27 or HSP70 inhibitors were administered, thrombi were significantly smaller than in the control group on day 5 after inferior vena cava ligation, indicating pro-thrombotic effects HSP27 and HSP70. If HSP27- or HSP70-positive cells were clearly visible and easily identifiable in the thrombus sections, the thrombus was presumed to be more than 10 days old. Thus, the detection of intrathrombotic HSP27 and HSP70 could forensically provide useful information for the estimation of thrombus ages. Collectively, our study implied that both HSP27 and HSP70 might be molecular targets for thrombus therapy and that the detection of HSP-related molecules such as HSP27 and HSP70 could be useful for the determination of thrombus ages.

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Mini Review: The Forensic Value of Heat Shock Proteins

Cited by 3 publications

References 29 publications

Prognosis value of heat-shock proteins in esophageal and esophagogastric cancer: A systematic review and meta-analysis

Prognosis value of heat-shock proteins in esophageal and esophagogastric cancer: A systematic review and meta-analysis

Forensic Application of Epidermal Ubiquitin Expression to Determination of Wound Vitality in Human Compressed Neck Skin

Relationship between intrathrombotic appearance of HSP27 and HSP70 and thrombus ages in a murine model of deep vein thrombosis

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