Miniaturized QCM-based flow system for immunosensor application in liquid

Michalzik, Monika; Wilke, Ralph; Büttgenbach, S.

doi:10.1016/j.snb.2005.03.048

Cited by 54 publications

(25 citation statements)

References 13 publications

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“…This result agrees well with a previous report. (16) The standard deviation of frequency depending on the flow rates is summarized in Table 1. Furthermore, by considering a sufficient reaction antigen volume and reaction time, the flow rate of 10 μl/min is selected for the following immunoreaction experiment.…”

Section: Resultsmentioning

confidence: 99%

“…The simple structure of the QCM and the development of a quartz micro-electromechanical system (MEMS) technique make it possible to achieve a miniaturized high-frequency QCM chip and resonator array. (13)(14)(15)(16) Recently, the fabrication and evaluation of a high-frequency fundamental QCM and the establishment of a portable flow-injection-based miniaturized QCM system have been reported in our previous publications. (17,18) The QCM system is composed of a microsyringe pump, a sample injector, a tailor-made flow cell, and a frequency monitoring tool.…”

Section: Introductionmentioning

confidence: 99%

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Development of Portable Quartz Crystal Microbalance for Biosensor Applications

2016

Sensors and Materials

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Keywords: quartz crystal microbalance, flow injection analysis, high frequency, biosensorIn this work, we report a novel flow-injection-type portable quartz crystal microbalance (QCM) sensor utilizing a high-frequency (higher than 40 MHz) miniaturized QCM chip. The resonator chip is assembled in a novel three-layer flow cell, in which the top and bottom layers are used to fix the spring-pin leading electrodes and the middle layer is used to house the resonator chip. A two-stage operational transconductance-amplifier-(OPA660)-based oscillator circuit is developed to drive the QCM and measure the frequency shift. A low human IgG concentration of 1 ppm has been measured even using the simple physical adsorption antibody immobilization method, which indicates that the developed portable QCM sensor can be used as a biosensor in liquids.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of Portable Quartz Crystal Microbalance for Biosensor Applications

2016

Sensors and Materials

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“…One good candidate to detect milk adulteration is by using quartz crystal microbalance (QCM). As shown in many works, the QCM sensor has been proved to be a good immunosensor [15][16][17][18][19][20]. Commercial quartz crystal resonator with polystyrene coating has been used successfully and developed as a basis for QCM Immunosensor [21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Development of QCM Biosensor with Specific Cow Milk Protein Antibody for Candidate Milk Adulteration Detection

et al. 2016

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Adulteration of goat milk is usually done using cow's milk product. Cow milk is used as it is widely available and its price is cheaper compared to goat milk. This paper shows a development of candidate tools for milk adulteration using cow milk. A quartz crystal microbalance immunosensor was developed using commercial crystal resonator and polyclonal antibody specific to cow milk protein. A specific protein at 208 KDa is found only in cow milk and does not exist in goat milk. The existence of this protein can be used as an indicator of cow milk content in a target solution. To detect the PSS 208 kDa protein, antibody specific to the PSS 208 was developed. The purified antibody was immobilized on top of the sensor surface on a polystyrene layer. The fraction of the immobilized antibody on the sensor was found at 1.5% of the given antibody. Using a static reaction cell, the developed immunosensor could detect the specific cow milk protein in buffer solution. The detection limit is 1 ppm. A linear relationship between frequency change and specific protein of cow milk concentration is found from a concentration of 1 ppm to 120 ppm.

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“…These requirements have not been met by either microtiter-based immunoassays, which can take hours, or by semiquantitative lateral flow strip assays. 9 Quartz crystal microbalance (QCM) sensors miniaturized to fit into microfluidic lab-on-chip systems 10 coupled with CRP-specific antibodies may provide a suitable alternative In this study, we generated CRP-specific single chain (sc) Fv antibodies, optimized immobilization strategies for their coupling onto the sensor chip and demonstrated their use for repeated measurement cycles RepoRt RepoRt two subsequent rounds were done using M13K07 packaged phagemids. Identification of binders was achieved by using ELISA of E. coli culture supernatants containing soluble scFv fragments on immobilized CRP.…”

Section: Introductionmentioning

confidence: 99%