Minimal microfabrication required digital microfluidic system toward point-of-care nucleic acid amplification test application for developing countries

Hoang, Trang; Ly, Bao-Han; Le, Thanh-Xuan; Huynh, Thanh-Thao; Nguyen, Hoang-Tuan; Vo, Toi Van; Pham, Thi-Thu-Hien; Huynh, Khon

doi:10.1007/s00542-019-04733-4

Cited by 10 publications

(7 citation statements)

References 27 publications

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“…routing capabilities to set up the electrode configuration (2-, 3-or 4-wire configuration). Compared to portable EWOD systems described before [34,36,37,40,43,44,46,47] PortaDrop is unique by using a Raspberry Pi and thus a Linux-based operating systems. Beside others, this feature provides the advantage of an easy adaption to a LAN environment as well as to the external devices.…”

Section: Discussionmentioning

confidence: 99%

“…In the original version, only 10 electrode pads are addressed but the develop board can control up to 46 channels at the same time. Only 45 V rms are needed as the droplet driving voltage because the bottom glass plate is coated with 1 μm thick cyanoethyl pullulan as a high-k dielectric layer [45] Recently, Hoang et al published a portable DMF platform consisting of four main components: a DMF chip, an Arduino-Nano-based control board, a user interface and a heating unit for loop-mediated isothermal amplification (LAMP) reactions [46]. The control board also incorporates a high-voltage DC-DC power converter, which is capable of stepping up to 275 V from a 12 V input.…”

Section: Plos Onementioning

confidence: 99%

“…The control board also incorporates a high-voltage DC-DC power converter, which is capable of stepping up to 275 V from a 12 V input. A 64-channel and 300 V-rating serial-to-parallel shift register HV507 (Microchip Technology Inc.) is used as an interface between the 5 V-compatible microcontroller and the high-voltage power line [46]. The HV507 allows for the control of up to 64 electrodes.…”

Section: Plos Onementioning

confidence: 99%

“…The HV507 allows for the control of up to 64 electrodes. Because the HV507 is not compatible with AC signals, a complicated solution is presented to address the electrodes individually [46]. A similar approach was published by Wan et al [47].…”

Section: Plos Onementioning

confidence: 99%

“…Recently, Hoang et al published a portable DMF platform consisting of four main components: a DMF chip, an Arduino-Nano-based control board, a user interface and a heating unit for loop-mediated isothermal amplification (LAMP) reactions [ 46 ]. The control board also incorporates a high-voltage DC-DC power converter, which is capable of stepping up to 275 V from a 12 V input.…”

Section: Introductionmentioning

confidence: 99%

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PortaDrop: A portable digital microfluidic platform providing versatile opportunities for Lab-On-A-Chip applications

et al. 2020

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Electrowetting-on-dielectric is a decent technique to manipulate discrete volumes of liquid in form of droplets. In the last decade, electrowetting-on-dielectric systems, also called digital microfluidic systems, became more frequently used for a variety of applications because of their high flexibility and reconfigurability. Thus, one design can be adapted to different assays by only reprogramming. However, this flexibility can only be useful if the entire system is portable and easy to use. This paper presents the development of a portable, standalone digital microfluidic system based on a Linux-based operating system running on a Raspberry Pi, which is unique. We present "PortaDrop" exhibiting the following key features: (1) an "all-in-one box" approach, (2) a user-friendly, self-explaining graphical user interface and easy handling, (3) the ability of integrated electrochemical measurements, (4) the ease to implement additional lab equipment via Universal Serial Bus and the General Purpose Interface Bus as well as (5) a standardized experiment documentation. We propose that PortaDrop can be used to carry out experiments in different applications, where small sample volumes in the nanoliter to picoliter range need to be handled an analyzed automatically. As a first application, we present a protocol, where a droplet is consequently exchanged by droplets of another medium using passive dispensing. The exchange is monitored by electrical impedance spectroscopy. It is the first time, the media exchange caused by passive dispensing is characterized by electrochemical impedance spectroscopy. Summarizing, PortaDrop allows easy combination of fluid handling by means of electrowetting and additional sensing.

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Section: Discussionmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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PortaDrop: A portable digital microfluidic platform providing versatile opportunities for Lab-On-A-Chip applications

et al. 2020

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Shape Programmable and Multifunctional Soft Textile Muscles for Wearable and Soft Robotics

Hoang,

Nguyen,

Phan

et al. 2024

Advanced Intelligent Systems

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Textiles are promising candidates for use in soft robots and wearable devices due to their inherent compliance, high versatility, and skin comfort. Planar fluidic textile‐based actuators exhibit low profile and high conformability, and can seamlessly integrate additional components (e.g., soft sensors or variable stiffness structures [VSSs]) to create advanced, multifunctional smart textile actuators. In this article, a new class of programmable, fluidic soft textile muscles (STMs) that incorporate multilayered silicone sheets with embedded fluidic channels is introduced. The STMs are scalable and fabricated by apparel engineering techniques, offering a fabrication approach able to create large‐scaled multilayered structures that can be challenging for current microfluidic bonding methods. They are also highly automation compatible due to no manual insertion of elastic tubes/bladders into textile structures. Liquid metal is employed for creating fluidic channels. It is not only used for actuation but also used as channels for additional features such as soft piezoresistive sensors with enhanced sensitivity to STMs’ pressure‐induced elongation, or VSSs of either low‐melting‐point alloys or a new thermo‐responsive epoxy with low viscosity and transition temperature. The STMs hold promising prospects for soft robotic and wearable applications, which is demonstrated by an example of a textile‐based wearable 3D skin‐stretch haptic interface.

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An integrated and automated digital microfluidic device for dairy milk droplet actuation

et al. 2023

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Minimal microfabrication required digital microfluidic system toward point-of-care nucleic acid amplification test application for developing countries

Cited by 10 publications

References 27 publications

PortaDrop: A portable digital microfluidic platform providing versatile opportunities for Lab-On-A-Chip applications

PortaDrop: A portable digital microfluidic platform providing versatile opportunities for Lab-On-A-Chip applications

Shape Programmable and Multifunctional Soft Textile Muscles for Wearable and Soft Robotics

An integrated and automated digital microfluidic device for dairy milk droplet actuation

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