Minimal requirements for the diagnosis, classification, and evaluation of the treatment of childhood acute lymphoblastic leukemia (ALL) in the “BFM family” cooperative group

Vanderdoesvandenberg, A; Bartram, CR; Basso, Giuseppe; Benoît, Yves; Biondi, Andrea; Debatin, Klaus‐Michael; Haas, OA; Harbott, Jochen; Kamps, WA; Köller, Ursula; Lampert, F.; Wd, Ludwig; Niemeyer, C.; Vanwering, Er

doi:10.1002/mpo.2950200603

Cited by 95 publications

(50 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ALL was diagnosed according to standard morphological, cytochemical and immunological criteria. 34 All cases were reviewed by the study center and the patients were treated according to the respective BFM therapy protocols after informed consent was obtained from either the patient's parents or legal guardians. Treatment stratification and protocols used in trials BFM-A 90 and BFM-A 95 have been published previously and response criteria as well as definition of relapse have been described in detail elsewhere.…”

Section: Patientsmentioning

confidence: 99%

Incidence and relevance of secondary chromosome abnormalities in childhood TEL/AML1+ acute lymphoblastic leukemia: an interphase FISH analysis

Attarbaschi

Mann

König³

et al. 2004

Leukemia

View full text Add to dashboard Cite

The aim of the present study was to determine the frequency and clinical relevance of the most common secondary karyotype abnormalities in TEL/AML1 þ B-cell precursor acute lymphoblastic leukemia (ALL) as assessed with fluorescence in situ hybridization (FISH) analyses. Screening of 372 patients who were enrolled in two consecutive Austrian childhood ALL multicenter trials identified 94 (25%) TEL/AML1 þ cases. TEL deletions, trisomy 21 and an additional der(21)t(12;21) were detected in 52 (55%), 13 (14%) and 14 (15%) TEL/AML1 þ patients, respectively. The 12p aberrations (P ¼ 0.001) and near tetraploidy (P ¼ 0.045) were more common in TEL/AML1 þ patients, whereas the incidence of diploidy, pseudodiploidy, hypodiploidy, low hyperdiploidy, near triploidy, del(6q), chromosome 9 and 11q23 abnormalities was similar among TEL/ AML1 þ and TEL/AML1À patients. None of the TEL/AML1 þ patients had a high hyperdiploid karyotype. Univariate analysis indicated that among TEL/AML1 þ patients those with a deletion of the nontranslocated TEL allele had a worse prognosis than those without this abnormality (P ¼ 0.034). We concluded that the type and incidence of the most common secondary aberrations in TEL/AML1 þ ALL can be conveniently identified with little additional effort during interphase screening with appropriate TEL and AML1 FISH probes. We also provided preliminary evidence that the deletion of the nontranslocated TEL allele may adversely influence the clinical course of TEL/AML1 þ ALL.

show abstract

Section: Patientsmentioning

confidence: 99%

Incidence and relevance of secondary chromosome abnormalities in childhood TEL/AML1+ acute lymphoblastic leukemia: an interphase FISH analysis

Attarbaschi

Mann

König³

et al. 2004

Leukemia

View full text Add to dashboard Cite

show abstract

“…The freshly obtained MNC samples were subjected to a detailed immunophenotyping according to standard protocols. [23][24][25] Remaining MNC were stored in liquid nitrogen. A leukemia was considered to be a precursor-B-ALL if the malignant cells were positive for terminal deoxynucleotidyl transferase (TdT), CD19 and HLA-DR (pro-B-ALL), for TdT, CD10, CD19 and HLA-DR (common ALL), or for TdT, CD10, CD19, HLA-DR and cytoplasmic Ig heavy-chain (CyIg ) (pre-B-ALL).…”

Section: Figurementioning

confidence: 99%

Cross-lineage T cell receptor gene rearrangements occur in more than ninety percent of childhood precursor-B acute lymphoblastic leukemias: alternative PCR targets for detection of minimal residual disease

et al. 1999

View full text Add to dashboard Cite

The discrepancies could be assigned to the presence of 'atypical' TCRD gene rearrangements or translocations only detectable by SB, but also to efficient PCR-based detection of rearrangements derived from small subclones, which are difficult to detect with SB. Indications for oligoclonality were observed in 38% and 30% of patients with TCRG and TCRD gene rearrangements, respectively, which is comparable to the frequency of oligoclonality in IGH locus. Based on the combined data it was possible to reduce the broad panel of six TCRD and 12 TCRG primer combinations for MRD studies to two TCRD combinations (V␦2-D␦3 and D␦2-D␦3) and six TCRG combinations (V␥I, V␥II, V␥IV family-specific primers with J␥1.1/2.1 and J␥1.3/2.3 primers) resulting in the detection of 80% and 97% of all TCRD and TCRG gene rearrangements, respectively. Finally, the heteroduplex PCR data indicate that MRD monitoring with TCRG and/or TCRD targets is possible in approximately 80% of childhood precursor-B-ALL patients; ෂ55% of patients even have two TCRG and/or TCRD targets.

show abstract

“…Diagnosis of ALL was made according to standard cytomorphology, cytochemistry and immunological marker analyses. 17 …”

Section: Patientsmentioning

confidence: 99%

Clonal stability in children with acute lymphoblastic leukemia (ALL) who relapsed five or more years after diagnosis

Nigro

Cazzaniga²,

Cataldo

et al. 1999

Leukemia

View full text Add to dashboard Cite

Although most relapses of childhood acute lymphoblastic leukemia (ALL) occur 24-36 months after first CR has been achieved, few patients relapse 5 or more years after CR achievement. The assessment of clonality has proved to be useful in determining whether even those very late events represent the reoccurrence of the original clone or alternatively a secondary leukemia. To gain further information on clonal stability in such late relapse, we performed detailed comparative Southern blotting and PCR analyses of TcR␦ and TcR␥ gene rearrangements in five ALL at presentation and subsequent relapse which occurred more than 5 years after diagnosis. At least one stable rearranged allele of the TcR␦ and TcR␥ loci was traced in all cases at presentation and clinical relapse despite a wide heterogeneity of the pattern of rearrangements. Our study extends to a larger series of patients previous findings which have sought to analyze the phenomenon of clonal evolution in children relapsed after more than 5 years of CCR. With respect to the potential pitfalls in monitoring minimal residual disease in childhood ALL for the presence of clonal evolution, our results highlight the combination of two target genes (such as TcR␥ and TcR␦) as a tool to reduce false negative MRD results.

show abstract

Minimal requirements for the diagnosis, classification, and evaluation of the treatment of childhood acute lymphoblastic leukemia (ALL) in the “BFM family” cooperative group

Cited by 95 publications

References 62 publications

Incidence and relevance of secondary chromosome abnormalities in childhood TEL/AML1+ acute lymphoblastic leukemia: an interphase FISH analysis

Incidence and relevance of secondary chromosome abnormalities in childhood TEL/AML1+ acute lymphoblastic leukemia: an interphase FISH analysis

Cross-lineage T cell receptor gene rearrangements occur in more than ninety percent of childhood precursor-B acute lymphoblastic leukemias: alternative PCR targets for detection of minimal residual disease

Clonal stability in children with acute lymphoblastic leukemia (ALL) who relapsed five or more years after diagnosis

Contact Info

Product

Resources

About