Minimum inhibitory concentrations of selected antimicrobial agents for<i>Moraxella bovoculi</i>associated with infectious bovine keratoconjunctivitis

Angelos, John A.; Ball, Louise M.; Byrne, Barbara A.

doi:10.1177/1040638711404154

Cited by 21 publications

(19 citation statements)

References 15 publications

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“…M. bovis strains presented a significant higher resistance against cloxacillin, cotrimoxazole and tetracycline compared to M. bovoculi. Although Angelos et al [21] found a 3.5% of resistant M. bovoculi to oxytetracycline, in our study all the isolates were sensitive against this antibiotic. The difference observed in resistant pattern against antibiotics between M. bovis and M. bovoculi may have implications in the pathogenicity and the treatment of IBK.…”

Section: Discussionmentioning

confidence: 45%

Diversity of Moraxella spp. strains recovered from infectious bovine keratoconjunctivitis cases in Uruguay

Sosa

Zunino

2013

J Infect Dev Ctries

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Background: Infectious bovine keratoconjunctivitis (IBK) is the most common ocular disease that affects cattle throughout the world and it has a very significant economic impact. IBK is caused by members of the genus Moraxella and therapeutic and preventive measures have shown limited success. Vaccines, most of them chemically inactivated bacterins, generally induce a limited protection. Methodology: In this study, the genetic diversity of Uruguayan clinical Moraxella bovis and Moraxella bovoculi isolates was assessed by RAPD-PCR, ERIC-PCR and BOX-PCR fingerprinting. Also, antibiotic resistance of the Moraxella spp. isolates was assessed utilizing the disk diffusion method. Results: When interspecific molecular diversity was assessed, different bands patterns were observed even within a single outbreak of IBK, showing the coexistence of different genotypes of Moraxella spp. The high genetic diversity within M. bovis and M. bovoculi isolates did not permit to correlate isolates DNA fingerprints with geographical origins, dates or even with both different Moraxella species. Antibiotics resistance patterns showed significant differences between M. bovis and M. bovoculi. Conclusions: This is the first study of diversity that includes M. bovis and M. bovoculi associated to IBK cases. Genetic diversity did not allow to correlate DNA fingerprints of the isolates with geographical origins, isolation dates or even both different Moraxella species. Antibiotics resistance patterns showed differences between M. bovis and M. bovoculi. This remarkable variation within isolates could explain the partial protection induced by commercial vaccines. All these findings could be important for the design of prevention or treatment strategies against IBK.

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Section: Discussionmentioning

confidence: 45%

Diversity of Moraxella spp. strains recovered from infectious bovine keratoconjunctivitis cases in Uruguay

Sosa

Zunino

2013

J Infect Dev Ctries

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“…The antimicrobial resistance patterns appear similar between geographically distinct M. bovis and M. bovoculi isolates, indicating that infections with either or both organisms show similar susceptibility patterns as previously noted. 5 However, it should be noted that there are some small differences, as the MIC 90 of M. bovoculi for oxytetracycline was higher than for M. bovis (4 µg/ml compared with 1 µg/ml).…”

Section: Discussionmentioning

confidence: 96%

Moraxellaspp. isolated from field outbreaks of infectious bovine keratoconjunctivitis: a retrospective study of case submissions from 2010 to 2013

Loy

Brodersen

2014

J VET Diagn Invest

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Infectious bovine keratoconjunctivitis (IBK), also known as pinkeye, is the most costly eye disease of cattle. The principal etiologic agent of IBK is the Gram-negative bacterium Moraxella bovis. However, there have been reports of IBK outbreaks associated with Moraxella bovoculi. A retrospective study of IBK diagnostic cases submitted from July 1, 2010 through October 31, 2013 was conducted. Included in the study were 1,042 Moraxella isolates from 1,538 swabs of lacrimal secretions collected from 282 herds from 30 U.S. states. Moraxella isolates were identified to the species level and were composed of M. bovoculi (701 isolates), M. bovis (295 isolates), Moraxella ovis (5 isolates), and other Moraxella spp. (41). Minimum inhibitory concentrations required for 90% growth inhibition (MIC90) was calculated for representative isolates. The MIC90 values for both M. bovis and M. bovoculi were as follows: ampicillin and ceftiofur: ≤0.25 µg/ml; clindamycin: 2 µg/ml; danofloxacin and enrofloxacin: ≤0.12 µg/ml; florfenicol: 0.5 µg/ml; gentamicin: 1 µg/ml; neomycin: 4 µg/ml; tulathromycin: 2 µg/ml; and tylosin: 8 µg/ml. The MIC90 values for M. bovoculi included the following: chlortetracycline: ≤0.5 µg/ml; oxytetracycline: 4 µg/ml; penicillin: 0.25 µg/ml; spectinomycin: 32 µg/ml; sulfadimethoxine: >256 µg/ml; tiamulin: 1 µg/ml; and trimethoprim-sulfamethoxazole: 4 µg/ml. For M. bovis, MIC90 values included the following: chlortetracycline and oxytetracycline: 1 µg/ml; penicillin: ≤0.12 µg/ml; spectinomycin: 16 µg/ml; sulfadimethoxine: ≤256 µg/ml; tiamulin: ≤0.5 µg/ml; and trimethoprim-sulfamethoxazole: ≤2 µg/ml. The current work describes the frequency of isolation and differences in antimicrobial sensitivity observed among Moraxella isolates from case submissions.

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“…Susceptibility testing was carried out as previously described 9. Streptococci and Pasteurella species were inoculated into cation-adjusted Mueller-Hinton broth containing laked horse blood before inoculation of Sensititre plates, sealed with perforated seals and incubated with 5 per cent CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Bacterial isolates and antimicrobial susceptibilities from odontogenic abscesses in rabbits: 48 cases

et al. 2017

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The medical record database of a veterinary teaching hospital was searched from 2000 through 2014 for records of client-owned rabbits with positive cultures from odontogenic abscesses. Data reviewed included sex, age, abscess location, sampling technique, previous antimicrobial treatments, microorganisms identified and antibiotic susceptibility of aerobic bacteria. Forty-eight client owned rabbits with one or more odontogenic abscesses and culture results were evaluated. One hundred and eighty-five isolates (52 aerobic, 133 anaerobic) were identified from 61 positive cultures from odontogenic abscesses. The most common aerobic bacterial isolates were (14/52), species (10/52), species (8/52) and species (7/52). The most common anaerobic bacterial isolates were species (36/133), species (27/133) and species (27/133). Mixed aerobic and anaerobic isolates were detected in 73.3 per cent of cultures and 50.8 per cent of the samples had greater than three isolates. was susceptible to amikacin and gentamicin, had variable resistance to enrofloxacin, and resistance to chloramphenicol. species, species and species isolates were susceptible to most antibiotics tested. The bacterial isolates from rabbit odontogenic abscesses are a mixture of aerobic and anaerobic Gram-positive and Gram-negative organisms and without culture and susceptibility testing, it is difficult to predict appropriate antimicrobial treatment.

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Minimum inhibitory concentrations of selected antimicrobial agents forMoraxella bovoculiassociated with infectious bovine keratoconjunctivitis

Cited by 21 publications

References 15 publications

Diversity of Moraxella spp. strains recovered from infectious bovine keratoconjunctivitis cases in Uruguay

Diversity of Moraxella spp. strains recovered from infectious bovine keratoconjunctivitis cases in Uruguay

Moraxellaspp. isolated from field outbreaks of infectious bovine keratoconjunctivitis: a retrospective study of case submissions from 2010 to 2013

Bacterial isolates and antimicrobial susceptibilities from odontogenic abscesses in rabbits: 48 cases

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