Mining Late Embryogenesis Abundant (LEA) Family Genes in Cleistogenes songorica, a Xerophyte Perennial Desert Plant

Muvunyi, Blaise Pascal; Qi, Yan; Wu, Fan; Min, Xueyang; Yan, Zhuan Zhuan; Kanzana, Gisele; Wang, Yanrong; Zhang, Jiyu

doi:10.3390/ijms19113430

Cited by 33 publications

(27 citation statements)

References 71 publications

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“…The correlative expression was calculated according to the ∆∆ Ct method. The expression quantity of CsGADPH gene was used as an internal control [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica

Zong

et al. 2020

Genes

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Plant-specific NAC (NAM, ATAF, CUC) transcription factor (TF) family plays important roles in biological processes such as plant growth and response to stress. Nevertheless, no information is known about NAC TFs in Cleistogenes songorica, a prominent xerophyte desert grass in northwestern China. In this study, 162 NAC genes were found from the Cleistogenes songorica genome, among which 156 C. songoricaNAC (CsNAC) genes (96.3%) were mapped onto 20 chromosomes. The phylogenetic tree constructed by CsNAC and rice NAC TFs can be separated into 14 subfamilies. Syntenic and Ka/Ks analyses showed that CsNACs were primarily expanded by genomewide replication events, and purifying selection was the primary force driving the evolution of CsNAC family genes. The CsNAC gene expression profiles showed that 36 CsNAC genes showed differential expression between cleistogamous (CL) and chasmogamous (CH) flowers. One hundred and two CsNAC genes showed differential expression under heat, cold, drought, salt and ABA treatment. Twenty-three CsNAC genes were commonly differentially expressed both under stress responses and during dimorphic floret development. Gene Ontology (GO) annotation, coexpression network and qRT-PCR tests revealed that these CsNAC genes may simultaneously regulate dimorphic floret development and the response to stress. Our results may help to characterize the NAC transcription factors in C. songorica and provide new insights into the functional research and application of the NAC family in crop improvement, especially in dimorphic floret plants.

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“…The correlative expression was calculated according to the ∆∆ Ct method. The expression quantity of CsGADPH gene was used as an internal control [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica

Zong

et al. 2020

Genes

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“…These proteins were found to accumulate at high concentrations during the last period of seed maturation, concurrent with dehydration 1,23 . More importantly, it has been demonstrated that their expression may be significantly induced under abiotic stress conditions, such as cold, heat, and drought 15,24,25 . It has been proposed that LEA proteins may be involved in various important functions against abiotic stresses, including the stabilization of membrane structures 2,26,27 , the scavenging free radicals 28,29 , and the sequestering ions 29,30 or biotin 31 etc.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses

Jin

Cao

Wang

et al. 2019

Sci Rep

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Late embryogenesis abundant (LEA) proteins are widely known to be present in higher plants and are believed to play important functional roles in embryonic development and abiotic stress responses. However, there is a current lack of systematic analyses on the LEA protein gene family in tea plant. In this study, a total of 48 LEA genes were identified using Hidden Markov Model profiles in C. sinensis, and were classified into seven distinct groups based on their conserved domains and phylogenetic relationships. Genes in the CsLEA_2 group were found to be the most abundant. Gene expression analyses revealed that all the identified CsLEA genes were expressed in at least one tissue, and most had higher expression levels in the root or seed relative to other tested tissues. Nearly all the CsLEA genes were found to be involved in seed development, and thirty-nine might play an important role in tea seed maturation concurrent with dehydration. However, only sixteen CsLEA genes were involved in seed desiccation, and furthermore, most were suppressed. Additionally, forty-six CsLEA genes could be induced by at least one of the tested stress treatments, and they were especially sensitive to high temperature stress. Furthermore, it was found that eleven CsLEA genes were involved in tea plant in response to all tested abiotic stresses. Overall, this study provides new insights into the formation of CsLEA gene family members and improves our understanding on the potential roles of these genes in normal development processes and abiotic stress responses in tea plant, particularly during seed development and desiccation. These results are beneficial for future functional studies of CsLEA genes that will help preserve the recalcitrant tea seeds for a long time and genetically improve tea plant.

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“…All the CsDehydrin proteins contained the YKS segments which are essential for keeping the capacity to adjust their conformation and maintain cellular homeostasis under stress conditions. Among the cis-regulatory elements of the LEA genes, more than three G-Boxes were registered for each dehydrin, LEA_2, and SMP genes and more than two MBS for dehydrin and LEA_2 subfamily gene [89].…”

Section: Findings Of Novel Genes In Native Plants From Arid Land Areasmentioning

confidence: 99%

Native Plants to Arid Areas: A Genetic Reservoir for Drought-Tolerant Crops

Trejo‐Calzada¹,

Pedroza‐Sandoval²,

Arreola-Ávila³

et al. 2020

Drought - Detection and Solutions

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Droughts are common in arid areas. These cause important losses in crop production, while the increasing population demands more food and goods. Cultivars able to produce under drought conditions are required to avoid or reduce production losses. Plants have evolved different mechanisms to face drought, and many genes have been already discovered in model and cultivated plants that are involved in this trait. Some of these genes have been successfully transformed into cultivated plants for drought tolerance. Plants native to arid lands may possess variants of drought tolerance mechanisms as compared to mesophytic or model plants. Also, different drought-related genes can be revealed. Studies using high-throughput and bioinformatic tools may allow to discover new genes and give new insights on the mechanisms involved in drought tolerance. However, still scarce studies in plants native to arid lands show that there are many drought-related genes that have not been already characterized and potentially they may be novel genes. These novel genes may be used to improve crops for drought tolerance. Therefore, more physiological, transcriptomic, proteomic, and metabolomic studies are needed on plants native to the deserts. protein rd22, pectinesterase-2, LEA protein, and POD. Moreover, 74 DEGs were commonly upregulated in leaf tissues and 16 were downregulated. The induced genes included pectinesterase-2, non-specific lipid-transfer protein, delta-1-pyrroline-5-carboxylate synthase, and ras-related protein rabc2a [90].

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Mining Late Embryogenesis Abundant (LEA) Family Genes in Cleistogenes songorica, a Xerophyte Perennial Desert Plant

Cited by 33 publications

References 71 publications

Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica

Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica

Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses

Native Plants to Arid Areas: A Genetic Reservoir for Drought-Tolerant Crops

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