Miniplatforms for Screening Biological Samples for KRAS and Four Mismatch Repair Proteins as New Tools for Fast Screening for Gastric and Colon Cancers

Staden, Raluca‐Ioana Stefan‐van; Bratei, Alexandru Adrian; Ilie-Mihai, Ruxandra-Maria; Gheorghe, Damaris-Cristina; Tuchiu, Bianca-Maria; Gurzu, Simona

doi:10.1149/1945-7111/acd358

Cited by 3 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared with the results obtained for the simultaneous assay of PMS2, MLH1, MSH2, MSH6, and KRAS reported using two miniplatforms based on stochastic microsensors designed using Nitrogen (9.3 %) and Boron (2.4 %) ‐ dopped graphene (NBDG) modified with frutafit HD and frutafit TEX, [21] the proposed stochastic microdisk can be also used in surgery rooms for on‐site tumoral tissue analysis, and also has better sensitivity for the assay of MSH2, MSH6, and KRAS, the SP‐based stochastic microdisk had a wider linear concentration range, and also a lower limit of determination. The reproducibility of stochastic microdisks design is higher than of the stochastic miniplatforms reported earlier [24] …”

Section: Resultsmentioning

confidence: 67%

“…The reproducibility of stochastic microdisks design is higher than of the stochastic miniplatforms reported earlier. [24] Screening tests of whole blood, saliva, urine, and tumoral tissues for MLH1, MSH2, MSH6, PMS2, and KRAS determination, using the stochastic microdisk based on SP and FT More than 200 samples of whole blood, saliva, urine, and tumoral tissues were screened using the proposed stochastic microdisks based on SP and FT. Molecular recognition of the biomarkers in the diagrams were done followed by the reading of the t on values and determination of the concentration of MLH1, MSH2, MSH6, PMS2, and of KRAS, accordingly with the stochastic method described above.…”

Section: Response Characteristics Of the Stochastic Microdisksmentioning

confidence: 99%

See 1 more Smart Citation

DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic Microdisks

Stefan‐van Staden,

Bratei,

Ilie‐Mihai

et al. 2023

ChemElectroChem

Self Cite

View full text Add to dashboard Cite

Two stochastic microdisks were designed and validated for the DNA mismatch repair assessment in gastric and colon cancers, through determining simultaneously the concentrations of MLH1, MSH2, MSH6, PMS2, and of KRAS in whole blood, urine, saliva, and tumoral tissues. The active surface of the stochastic microdisks was composed of a thin layer of graphene paste decorated with nitrogen, boron, and sulfur and modified with inutec (SP) and with frutafit (FT), respectively. High sensitivities were recorded when the SP was used as modifier. All limits of determination were of fg mL−1 magnitude order. The paired student‐t‐test done at 99.00 % confidence level revealed that there are no significative differences between the results obtained using the two stochastic microdisks. Validation of the screening tests of whole blood, urine, saliva, and tumoral tissues was also done using the recovery tests when % recovery was higher than 87.00 %, with %RSD values lower than 1.00 %.

show abstract

Section: Resultsmentioning

confidence: 67%

Section: Response Characteristics Of the Stochastic Microdisksmentioning

confidence: 99%

DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic Microdisks

Stefan‐van Staden,

Bratei,

Ilie‐Mihai

et al. 2023

ChemElectroChem

Self Cite

View full text Add to dashboard Cite

show abstract

“…Stochastic sensors are good candidates as tools for biomedical analysis: [7][8][9][10] no sampling is needed for the biological sample; extremely small concentrations (attog/ml and lower) may be determined from very complex matrices; the composition of the matrix does not influence in any way the results of the analysis; a molecular recognition of the biomarkers is possible, based on their signatures; quantitative analysis is highly reliable, and the matrix did not influenced it. Therefore, two types of stochastic sensors were chosen as recognition and analysis tools for the molecular recognition and quantification of VSIG1 in biological samples such as: whole blood, urine, saliva, and tumoral tissues.…”

mentioning

confidence: 99%

Molecular Recognition of VSIG1 in Biological Samples for Fast Diagnosis of Gastric Cancer

Stefan-van Staden,

Gheorghe,

Ilie-Mihai

2024

J. Electrochem. Soc.

Self Cite

View full text Add to dashboard Cite

VSIG1 is a new biomarker member of the JAM family relevant in gastric cancer diagnostics. Due to its detection and quantification impact for fast and early diagnosis of gastric cancer, two types of intelligent miniplatforms based on stochastic sensors as detection tools, were designed and validated using real samples. A 3D stochastic microsensor based on Nitrogen and Sulfur dopped graphene paste modified with calix[4]arene-25,26,27,28-tetrol, and a 2D disposable screen-printed stochastic sensor based on thin film gold modified with calix[4]arene-25,26,27,28-tetrol were constructed and inserted as working sensors into the miniplatforms. The proposed intelligent miniplatforms shown sensitivities as high as 1.121010 s-1g-1mL, limits of determination of 110-23g mL-1, and working concentration ranges between 110-23 and 110-8g mL-1. Recoveries higher than 99.30% with % RSD values lower than 0.05% were obtained when used for screening test of biological samples, for VSIG1.

show abstract

Correlations between MSH2 and MSH6 Concentrations in Different Biological Fluids and Clinicopathological Features in Colorectal Adenocarcinoma Patients and Their Contribution to Fast and Early Diagnosis of Colorectal Adenocarcinoma

Bratei,

Stefan-van Staden

2023

Biomedicines

Self Cite

View full text Add to dashboard Cite

(1) Background: The human MutS homolog, hMSH2, is known to be involved in DNA mismatch repair and is responsible for maintaining the stability of the genome. When DNA damage occurs, MSH2 promotes cell apoptosis via the regulation of ATR/Chk2/p53 signal transduction, and MSH2 deficiency is also related to accelerated telomere shortening in humans. MSH2 missense mutations are involved in a defective DNA reparation process, and it can be implied in carcinogenesis, as it is already involved in well-known cancer-related syndromes such as Lynch syndrome. Human MSH6, which stands for mutS homolog 6, is a member of the MMR family that is responsible for the repair of post-replicative mismatched DNA bases. It is also one of the proteins with gene mutations that are associated with a high risk of developing Lynch syndrome, leading to a large series of tumors. (2) Methods: Patients and their clinical and pathological features were selected from the database of the project GRAPHSENSGASTROINTES and used accordingly, with ethics committee approval no. 32647/2018 awarded by the County Emergency Hospital from Targu-Mures. Analyses were conducted on whole blood, saliva, urine, and tumoral tissue samples using a stochastic method with stochastic microsensors. (3) Results: The results obtained using stochastic sensors were correlated with a series of macroscopic and microscopic pathological features for each sample type. Criteria or relationships were established for tumor location, vascular and perineural invasions, lymph node metastases, the presence of tumor deposits, and the presence of a mucus compound in the tumor mass. (4) Conclusions: The correlation between the concentrations of MSH2 in the four types of samples and the pathological features allowed for the fast characterization of a tumor, which can help surgeons and oncologists choose personalized treatments. Also, the colorectal tumor location was correlated with the concentration of MSH2 in whole blood, urine, and saliva. MSH6, which stands for mutS homolog 6, is not only useful in immunohistochemistry but in pathology practice as well. In this paper, the relationships between MSH6 levels in four biological fluids—whole blood, saliva, urine, and tissues—and tumor locations among the colorectal area, gross features, presence of a mucinous compound, molecular subtype, stroma features, and vascular invasions are presented.

show abstract

Miniplatforms for Screening Biological Samples for KRAS and Four Mismatch Repair Proteins as New Tools for Fast Screening for Gastric and Colon Cancers

Cited by 3 publications

References 39 publications

DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic Microdisks

DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic Microdisks

Molecular Recognition of VSIG1 in Biological Samples for Fast Diagnosis of Gastric Cancer

Correlations between MSH2 and MSH6 Concentrations in Different Biological Fluids and Clinicopathological Features in Colorectal Adenocarcinoma Patients and Their Contribution to Fast and Early Diagnosis of Colorectal Adenocarcinoma

Contact Info

Product

Resources

About