MIR-1275 promotes cell migration, invasion and proliferation in squamous cell carcinoma of head and neck via up-regulating igf-1R and CCR7

Liu, M.D.; Wu, Hsien‐Tsai; Wang, S.; Pang, Pai; Jin, Shan; Sun, Chengyi; Liu, F.Y.

doi:10.1016/j.ijom.2019.03.242

Cited by 7 publications

(11 citation statements)

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“…Over the past decades, the emerging role of ncRNAs, including miRNAs, have been reported in the development of EC radioresistance. [28][29][30][31] In this study, we proved that miR-1275, which has been revealed to exert paradoxical functions in different cancer types, 12,[32][33][34] was down-regulated in radioresistant EC cells. Meanwhile, we illustrated that miR-1275 could increase the sensitivity of EC cells to radiotherapy both in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 65%

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MicroRNA‐1275 induces radiosensitization in oesophageal cancer by regulating epithelial‐to‐mesenchymal transition via Wnt/β‐catenin pathway

Xie

You-yi

Fei

et al. 2019

J Cellular Molecular Medi

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Acquired radioresistance is one of the main obstacles for the anti‐tumour efficacy of radiotherapy in oesophageal cancer (EC). Recent studies have proposed microRNAs (miRNAs) as important participators in the development of radioresistance in various cancers. Here, we investigated the role of miR‐1275 in acquired radioresistance and epithelial‐mesenchymal transition (EMT) in EC. Firstly, a radioresistant cell line KYSE‐150R was established, with an interesting discovery was observed that miR‐1275 was down‐regulated in KYSE‐150R cells compared to the parental cells. Functionally, miR‐1275 inhibition elevated radioresistance in KYSE‐150 cells via promoting EMT, whereas enforced expression of miR‐1275 increased radiosensitivity in KYSE‐150R cells by inhibiting EMT. Mechanically, we demonstrated that miR‐1275 directly targeted WNT1 and therefore inactivated Wnt/β‐catenin signalling pathway in EC cells. Furthermore, WNT1 depletion countervailed the promoting effect of miR‐1275 suppression on KYSE‐150 cell radioresistance through hampering EMT, whereas WNT1 overexpression rescued miR‐1275 up‐regulation‐impaired EMT to reduce the sensitivity of KYSE‐150R cells to radiation. Collectively, our findings suggested that miR‐1275 suppressed EMT to encourage radiosensitivity in EC cells via targeting WNT1‐activated Wnt/β‐catenin signalling, providing a new therapeutic outlet for overcoming radioresistance of patients with EC.

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Section: Discussionmentioning

confidence: 65%

“…11 Conversely, miR-1275 promotes the progression in head and neck squamous cell carcinoma through up-regulating IGF-1R and CCR7. 12 Importantly, the down-regulation of miR-1275 in radioresistant EC cells has already been proven previously. 13 However, the precise function of miR-1275 in EC remains to be identified.…”

mentioning

confidence: 80%

MicroRNA‐1275 induces radiosensitization in oesophageal cancer by regulating epithelial‐to‐mesenchymal transition via Wnt/β‐catenin pathway

Xie

You-yi

Fei

et al. 2019

J Cellular Molecular Medi

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“…For instance, it has been shown that miR-1275 regulates the proliferation and invasion of lung cancer cells by downregulation of LZTS3 [10]. Liu et al have shown that miR-1275 accelerates the proliferation and migration of head and neck squamous cell carcinoma cells [11]. miR-1275 modulates the proliferation of nasopharyngeal cancer cells by targeting HOXB5 [12].…”

Section: Discussionmentioning

confidence: 99%

“…The intercommunication between different cells via exosomal miRNAs is suggested to be an important way of interaction between tumor cells and the tumor microenvironment [9]. Multiple studies have been demonstrated that miR-1275 plays an important role in cancer progression [10][11][12]. Using the whole miRNome analysis, Nam et al have shown that miR-1275 is a potential candidate associated with PCa metastasis [13].…”

Section: Introductionmentioning

confidence: 99%

Prostate Cancer-Secreted Exosomal miR-1275 Modulates Osteoblast Proliferation and Differentiation by Targeting SIRT2/RUNX2 Cascade

Zou¹,

Dai²,

Deng³

et al. 2020

Preprint

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BackgroundCancer-derived exosomes can promote tumor metastasis by delivering miRNAs from cancer cells to recipient cells. miR-1275 is suggested to be an important regulator involved in prostate cancer (PCa) metastasis; however, the association of miR-1275 with the microenvironment of PCa bone metastasis is unclear.Materials and methodsThe level of relative genes and proteins were detected by qPCR and western-blot, cell ability were examined by CCK-8 , the identification of target gene was analyzed by dual-luciferase reporter assay,exosomes were isolated from the PC3-derived conditioned medium by ultracentrifugation.Resultwe found that miR-1275 could be transferred from PCa cells to osteoblasts via exosomes. Exosomal miR-1275 significantly accelerated the proliferation of osteoblasts and the expression levels of osteoblast-specific genes, such as osteocalcin (OCN), type I collagen (COL-1), and osteopontin (OPN). Moreover, exosomal miR-1275 increased the expression of RUNX2, a master modulator of osteoblast differentiation, by down-regulation of SIRT2, which in turn influenced the proliferation and differentiation of osteoblasts.ConclusionsOur findings indicate that exosomal miR-1275 is a promoter of osteoblast activity, which may contribute to PCa bone metastasis.

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“…In this study, these miRNAs were found to be downregulated in tumor cells but only miR-1275 was focused and their putative target genes were newly explored in para n embedded BC tissues. Several researches have reported that this miRNA is down-expressed in gastric and nasopharyngeal carcinoma and function as a tumor suppressor (34)(35)(36); whereas, this miRNA is overexpressed in non-small lung cell cancer, squamous carcinoma and chronic myelogenous leukemia (37)(38)(39).…”

Section: Discussionmentioning

confidence: 99%

Identification of Tumor-Suppressive miRNA-1275 as a Novel Marker for Breast Cancer (BC) by MACE-Sequencing and RT-qPCR Techniques

Majed

Mustafa

2020

Preprint

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IntroductionDisruption of cellular processes in the breast by abnormally expressed miRNA is characterized to develop cancer. We aimed to determine the differential expression of coding and non-coding RNAs in formalin fixed paraffin embedded (FFPE) blocks of breast cancer (BC) tissue and normal adjacent tissue (NAT). Another aim is to determine differential expression of has-miR-1275 as novel biomarker for BC and identify its target genes using prediction sites and experimentally expression level of them via the MACE-sequencing technique. MethodsMACE-sequencing technique was utilized to analyze differential expression of coding RNAs and small RNAs (sRNAs). Among small RNAs, miRNA-1275 expression was focused and confirmed using RT-qPCR technique in 20 Kurdish cases with BC . Moreover, clinical significance of miR- 1275 and its target genes was studied in a large number of patients with BC using the data obtained from The Cancer Genome Atlas database.ResultsThe MACE-seq findings showed that 1400 sRNAs and 26843 coding RNAs were differentially expressed in FFPE of BC tissue compared to NAT. Among these sRNAs, miRNA-1275 expression was found to be decreased in BC tissue compared to NAT. The decreased expression level of which was then confirmed via RT-qPCR technique to farther prove in 20 Kurdish cases with BC . Furthermore, the correlation between the expression level of miRNA-1275 and clinical data were evaluated to be highly corrected in cases with BC (overall survival rate: P = 0.0401). However, putative target genes ( DVL3, PPP 2R2D, THSD4, CREB1, SYT7, and PRKACA) were computationally identified as direct targets of miRNA-1275 in several target predicted sites. Among coding RNAs, the expression level of these targets was increased in BC tissue compared to NAT. The levels of these targets were negatively associated with miRNA-1275 expression. Finally, the role of down-expressed miRNA-1275 and its targets in BC cells were identified to attenuated biological mechanisms; including cell growth, proliferation, movement, invasion, metastasis, and apoptosis.Conclusiondown-expressed miR-1275 , a tumor suppressor, is as a novel biomarker for early detection of breast cancer. DVL3, PPP 2R2D, THSD4, CREB1, SYT7, and PRKACA are novely identified to be targeted by miR-1275 in BC cells.

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MIR-1275 promotes cell migration, invasion and proliferation in squamous cell carcinoma of head and neck via up-regulating igf-1R and CCR7

Cited by 7 publications

References 0 publications

MicroRNA‐1275 induces radiosensitization in oesophageal cancer by regulating epithelial‐to‐mesenchymal transition via Wnt/β‐catenin pathway

MicroRNA‐1275 induces radiosensitization in oesophageal cancer by regulating epithelial‐to‐mesenchymal transition via Wnt/β‐catenin pathway

Prostate Cancer-Secreted Exosomal miR-1275 Modulates Osteoblast Proliferation and Differentiation by Targeting SIRT2/RUNX2 Cascade

Identification of Tumor-Suppressive miRNA-1275 as a Novel Marker for Breast Cancer (BC) by MACE-Sequencing and RT-qPCR Techniques

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