MiR‐1290 promotes proliferation, migration, and invasion of glioma cells by targeting <i>LHX6</i>

Liu, Yan; Cai, Kerui; Sun, Kai; Gui, Jinqiu; Liang, Jun

doi:10.1002/jcp.26381

Cited by 44 publications

(35 citation statements)

References 20 publications

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“…Further selecting HepG2 cells with the highest expression level of miR-1290 in liver cancer cells to extract exosomes miR-1290 was carried out for subsequent tests, it was found that the proliferation, invasion, migration and epithelial-mesenchymal transformation capabilities of liver cancer cells co-cultured with exosomes miR-1290 were significantly increased, while the apoptosis capability was significantly reduced, suggesting that miR-1290 plays a role of oncogene in liver cancer, while exosomes miR-1290 could enhance its tumor activity. Looking up relevant literature, we found that Yan et al [24] were also studying the role of miR-1290 as a tumor-promoting gene in glioma, which also confirmed the consistency of the biological effects of miR-1290.…”

Section: Discussionsupporting

confidence: 73%

Exosome-mediated miR-1290 regulation of SLU7 affects hepatocellular carcinoma process

Fan

Zhang

et al. 2020

Preprint

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Objective: Liver cancer, as a very common malignant tumor in clinic, has a high incidence rate and a poor prognosis. Understanding the relationship of exosomes miR-1290 and SLU7 with liver cancer may be a breakthrough in the diagnosis and treatment of liver cancer in the future.Methods: Altogether 64 patients with liver cancer and 58 healthy people in Shandong Cancer Hospital affiliated to Shandong University were collected, and liver cancer cells were purchased. qPCR was used to detect the mRNA levels of miR-1290 and SLU7. Western blot was used to detect the relative expression of SLU7 protein, and the effect of exosomes miR-1290 and SLU7 on the biological behavior of liver cancer cells was analyzed. Double fluorescein reporter was used to verify the targeted relationship between miR-1290 and SLU7.Results: miR-1290 was highly expressed in liver cancer (P< 0.050) and SLU7 was poorly expressed in liver cancer (P< 0.050). Exosomes miR-1290 could inhibit the relative expression of SLU7 protein (p < 0.050), promote the proliferation, invasion, migration and epithelial-mesenchymal transformation of liver cancer cells, and reduce the ability of apoptosis (P< 0.050). Double luciferase report and rescue experiment confirmed that miR-1290 and SLU7 had targeted relationship (P< 0.050).Conclusion: Exosomes miR-1290 may be a potential target for the diagnosis and treatment of liver cancer in the future through targeting inhibition of SLU7 in the occurrence and development of liver cancer.

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Section: Discussionsupporting

confidence: 73%

Exosome-mediated miR-1290 regulation of SLU7 affects hepatocellular carcinoma process

Fan

Zhang

et al. 2020

Preprint

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“…Further selecting HepG2 cells with the highest expression level of miR-1290 in liver cancer cells to extract exosomes miR-1290 was carried out for subsequent tests, it was found that the proliferation, invasion, migration and epithelial-mesenchymal transformation capabilities of liver cancer cells co-cultured with exosomes miR-1290 were signi cantly increased, while the apoptosis capability was signi cantly reduced, suggesting that miR-1290 plays a role of oncogene in liver cancer, while exosomes miR-1290 could enhance its tumor activity. Looking up relevant literature, we found that Yan et al 24 were also studying the role of miR-1290 as a tumor-promoting gene in glioma, which also con rmed the consistency of the biological effects of miR-1290.…”

Section: Discussionsupporting

confidence: 56%

Exosome-mediated miR-1290 regulation of SLU7 affects hepatocellular carcinoma process

Zeng

Tan

Gong

et al. 2020

Preprint

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Objective: Liver cancer, as a very common malignant tumor in clinic, has a high incidence rate and a poor prognosis. Understanding the relationship of exosomes miR-1290 and SLU7 with liver cancer may be a breakthrough in the diagnosis and treatment of liver cancer in the future.Methods: Altogether 64 patients with liver cancer and 58 healthy people in our hospital were collected, and liver cancer cells were purchased. qPCR was used to detect the mRNA levels of miR-1290 and SLU7. Western blot was used to detect the relative expression of SLU7 protein, and the effect of exosomes miR-1290 and SLU7 on the biological behavior of liver cancer cells was analyzed. Double fluorescein reporter was used to verify the targeted relationship between miR-1290 and SLU7.Results: miR-1290 was highly expressed in liver cancer (P< 0.050) and SLU7 was poorly expressed in liver cancer (P< 0.050). Exosomes miR-1290 could inhibit the relative expression of SLU7 protein (p < 0.050), promote the proliferation, invasion, migration and epithelial-mesenchymal transformation of liver cancer cells, and reduce the ability of apoptosis (P< 0.050). Double luciferase report and rescue experiment confirmed that miR-1290 and SLU7 had targeted relationship (P< 0.050).Conclusion: Exosomes miR-1290 may be a potential target for the diagnosis and treatment of liver cancer in the future through targeting inhibition of SLU7 in the occurrence and development of liver cancer.

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“…Among the top-regulated miRNAs was miR-210, known to respond to hypoxia and with many documented roles in cancer [23]. Here, we identified a new hypoxia-associated miRNA, miR-1290, which promotes cell migration and invasion of cancer cells [69,70]. In addition, EV miR-1290 was found to be a biomarker in ovarian carcinoma [71].…”

Section: Discussionmentioning

confidence: 87%

Hypoxia-Induced Adaptations of miRNomes and Proteomes in Melanoma Cells and Their Secreted Extracellular Vesicles

Walbrecq

Lecha

Gaigneaux

et al. 2020

Cancers

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Reduced levels of intratumoural oxygen are associated with hypoxia-induced pro-oncogenic events such as invasion, metabolic reprogramming, epithelial–mesenchymal transition, metastasis and resistance to therapy, all favouring cancer progression. Small extracellular vesicles (EV) shuttle various cargos (proteins, miRNAs, DNA and others). Tumour-derived EVs can be taken up by neighbouring or distant cells in the tumour microenvironment, thus facilitating intercellular communication. The quantity of extracellular vesicle secretion and their composition can vary with changing microenvironmental conditions and disease states. Here, we investigated in melanoma cells the influence of hypoxia on the content and number of secreted EVs. Whole miRNome and proteome profiling revealed distinct expression patterns in normoxic or hypoxic growth conditions. Apart from the well-known miR-210, we identified miR-1290 as a novel hypoxia-associated microRNA, which was highly abundant in hypoxic EVs. On the other hand, miR-23a-5p and -23b-5p were consistently downregulated in hypoxic conditions, while the protein levels of the miR-23a/b-5p-predicted target IPO11 were concomitantly upregulated. Furthermore, hypoxic melanoma EVs exhibit a signature consisting of six proteins (AKR7A2, DDX39B, EIF3C, FARSA, PRMT5, VARS), which were significantly associated with a poor prognosis for melanoma patients, indicating that proteins and/or miRNAs secreted by cancer cells may be exploited as biomarkers.

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MiR‐1290 promotes proliferation, migration, and invasion of glioma cells by targeting LHX6

Cited by 44 publications

References 20 publications

Exosome-mediated miR-1290 regulation of SLU7 affects hepatocellular carcinoma process

Exosome-mediated miR-1290 regulation of SLU7 affects hepatocellular carcinoma process

Exosome-mediated miR-1290 regulation of SLU7 affects hepatocellular carcinoma process

Hypoxia-Induced Adaptations of miRNomes and Proteomes in Melanoma Cells and Their Secreted Extracellular Vesicles

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