MiR-133b Is Down-Regulated in Human Osteosarcoma and Inhibits Osteosarcoma Cells Proliferation, Migration and Invasion, and Promotes Apoptosis

Zhao, Hua; Li, Mei; Li, Lihua; Yang, Xiaoming; Lan, Guobo; Zhang, Yu

doi:10.1371/journal.pone.0083571

Cited by 95 publications

(71 citation statements)

References 46 publications

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“…Furthermore, enhanced miR-125 expression was shown to reduce MCL1 expression and inhibit invasion of a human gastric cancer cells (54). Similarly, targeting of MCL1 by miR-107 was shown to abrogate invasion of cervical cancer-derived SiHa and HeLa cell lines (55), and miR-133b was shown to knockdown MCL1 expression in two osteosarcoma cell line lines, thereby reducing their invasive capacity in vitro (56). Therefore, these MCL-1-targeting miRNAs are interesting candidates to be validated for their modulating effects on MCL1 expression also in melanoma cells.…”

Section: Discussionmentioning

confidence: 92%

miR-339-3p Is a Tumor Suppressor in Melanoma

et al. 2016

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Determinants of invasion and metastasis in cancer remain of great interest to define. Here, we report the definition of miR-339-3p as a novel tumor suppressive microRNA that blocks melanoma cell invasion without affecting cell survival. miR-339-3p was identified by a comprehensive functional screen of a human miRNA mimetic library in a cell-based assay for invasion by the melanoma cell line A375. miR-339-3p was determined as a strong inhibitor of invasion differentially expressed in melanoma cells and healthy melanocytes. MCL1 was defined as a target for downregulation by miR-339-3p, functioning through direct interaction with the 3 0 untranslated region of MCL1 mRNA. Blocking miR-339-3p by an antagomiR was sufficient to increase melanoma cell invasion, an effect that could be phenocopied by RNAi-mediated silencing of MCL1. In vivo studies established that miR-339-3p overexpression was sufficient to decrease lung colonization by A375 melanoma cells in NSG mice, relative to control cells. Overall, our results defined miR-339-3p as a melanoma tumor suppressor, the levels of which contributes to invasive aggressiveness.

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Section: Discussionmentioning

confidence: 92%

miR-339-3p Is a Tumor Suppressor in Melanoma

et al. 2016

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“…Evidences have indicated that miRNAs play critical roles in managing many normal biological processes, and abnormal expression of miRNAs is associated with various cancers occurrence, such as gas tric cancer, lung cancer, OS, etc. (12)(13)(14), and miRNAs serve as oncogenes or cancer suppressors during the progression of OS (15,16). For example, Bi et al (17) suggested that miR-100 serves as a cancer inhibitor in OS development via suppressing OS growth by regulating FGFR3 expression.…”

Section: Introductionmentioning

confidence: 99%

“…MiR -663b has been found to be up-regulated in OS (14), however, the exact roles of miR-663b in OS and the underlying molecular mechanisms are still unclear. Thus, in this study, we will investigate the role of miR-663b in OS and further explore the underlying molecular mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Blockade of miR-663b inhibits cell proliferation and induces apoptosis in osteosarcoma via regulating TP73 expression

Shu¹,

Ye²,

Gu³

et al. 2018

BLL

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OBJECTIVE: This study aimed to investigate the exact role of miR-663b in osteosarcoma (OS) progression and further explore the underlying molecular mechanisms. MATERIALS AND METHODS: The miR-663b expression in human OS cell lines was determined by qRT-PCR, and the results suggested that miR-663b was highly expressed in human OS cells. TargetScan was used to predict the potential targets of miR-663b, and the prediction was confi rmed by dual-luciferase reporter assay. To investigate the role of miR-663b in OS, miR-663b was down-regulated in U2OS cells using miR-663b inhibitor. CCK8 and fl ow cytometry were preformed to investigate the proliferation and apoptosis of U2OS cells. Moreover, qRT-PCR and western blot analysis were performed to measure the mRNA and protein expression. RESULTS: We found that miR-663b directly targets TP73 and negatively regulates TP73 expression. MiR-663b inhibitor signifi cantly decreased the proliferation ability of U2OS cells, while the percentage of apoptotic cells was markedly increased. The level of Bcl-2 was notably inhibited by miR-663b inhibitor, while Bax expression was signifi cantly enhanced. Moreover, miR-663b down-regulation promoted p53 and p21 expression in U2OS cells. CONCLUSIONS: MiR-663b down-regulation represses proliferation and induces apoptosis in OS by targeting TP73. Therefore, we provide a potential therapeutic target for OS treatment (Fig. 6, Ref. 27). Text in PDF www.elis.sk.

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“…In contrast, the expression of miR-133a and miR133b in osteosarcoma is lower than healthy human skeletal muscle samples. Overexpression of miR-133b reduces tumor cell proliferation, migration, invasion and induces apoptosis in osteosarcoma cell lines, indicating a tumor suppressor role of miR-133b in osteosarcoma [77] . Recently, mature miRNAs labeled with unique DNA tags have been profiled in human sarcomas and normal tissues.…”

Section: Mirna Analysismentioning

confidence: 99%

The advancements of genomics and data integration in sarcoma research

et al. 2017

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Abstract:In the age of big data, genomics and clinical research have reached a crossroads. A wealth of data is being generated, but it is becoming increasingly complicated to analyze these data to extract meaningful results. The ability to understand biological systems holistically has unprecedented potential to transform how cancers are treated. Recent major advances leading biomedical research towards "systems medicine" have been fueled by high-throughput platforms, such as microarrays and next-generation sequencing, which can capture vast amounts of data in different genomic spaces. Unfortunately, because of high dimensionality and complex relationships among these data, inferring comprehensive and useful biological models has proven computationally and statistically challenging. However, novel bioinformatic methods for data integration of cancer genomic datasets have been developed. In this review, we will describe the applications of various genomic approaches in sarcoma research and introduce bioinformatic methods for data integration. With the continuing evolution of technological and bioinformatic methodologies, the application of big data within clinics and hospitals will ultimately result in significant improvements on how cancers are detected and treated.Keywords: omics; genomics; sarcomas; sequencing; microarray; data integration; bioinformatics; big data

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MiR-133b Is Down-Regulated in Human Osteosarcoma and Inhibits Osteosarcoma Cells Proliferation, Migration and Invasion, and Promotes Apoptosis

Cited by 95 publications

References 46 publications

miR-339-3p Is a Tumor Suppressor in Melanoma

miR-339-3p Is a Tumor Suppressor in Melanoma

Blockade of miR-663b inhibits cell proliferation and induces apoptosis in osteosarcoma via regulating TP73 expression

The advancements of genomics and data integration in sarcoma research

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