miR-142-5p regulates CD4+ T cells in human non-small cell lung cancer through PD-L1 expression via the PTEN pathway

Wan, Jun; Ling, Xiean; Peng, Bin; Ding, Guanggui

doi:10.3892/or.2018.6439

Cited by 35 publications

(31 citation statements)

References 26 publications

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“…miR-21 targets Pten. Pten promotes the proliferation of T cells and tumor cells, and has a critical role in ranKl-induced oc differentiation (15,16). in the present study, target genes that were potentially regulated by mir-21 were predicted by TargetScan and mirTarBase; Pten, which contains mir-21 binding sites in its 3'uTr, was selected as one of the target genes of mir-21.…”

Section: Expression Of Mir-21 Is Upregulated During Osteoclastogenesismentioning

confidence: 99%

miR‑21 promotes osteoclastogenesis through activation of PI3K/Akt signaling by targeting Pten in RAW264.7 cells

et al. 2020

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The present study aimed to investigate the effects of microrna (mir)-21 on osteoclastogenesis and its underlying molecular mechanisms. The expression levels of tartrate-resistant acid phosphatase (TraP) and mir-21 were detected during osteoclastogenesis in receptor activator of nF-κB ligand (ranKl)-induced raW264.7 cells via reverse transcription-quantitative Pcr. Bioinformatics and dual luciferase reporter assays were performed to analyze the association between mir-21 and Pten. ranKl-induced raW264.7 cells were divided into the following groups: Mir-negative control (nc), mir-21 mimic, mir-21 inhibitor and mir-21 mimic + lY294002. The effects of mir-21 on osteoclastogenesis and bone resorption were then detected using TraP staining and a bone resorption assay. Pten, phosphorylated-akt and nuclear factor of activated T cell (nFaTc1) expression levels were measured by western blotting to analyze the effects of mir-21 on the Pi3K/akt signaling pathway. The present study revealed that mir-21 was upregulated during osteoclastogenesis in ranKl-induced raW264.7 cells. Furthermore, mir-21 negatively regulated Pten. compared with the mir-negative control (nc) group, the number of osteoclasts and the percentage of bone resorption were increased in the mir-21 mimic group, whereas they were decreased in the mir-21 inhibitor group. The number of osteoclasts and the percentage of bone resorption in the mir-21 mimic + lY294002 group were lower than in the mir-21 mimic group. compared with the mir-nc group, the protein expression levels of Pten were decreased, whereas p-akt and nFaTc1 were increased in the mir-21 mimic group. conversely, Pten protein expression was increased, whereas p-akt and nFaTc1 were decreased in the mir-21 inhibitor group. in the mir-21 mimic + lY294002 group, Pten protein expression was higher, and p-akt and nFaTc1 were lower than in the mir-21 mimic group. in conclusion, mir-21 is upregulated during osteoclastogenesis, and may promote osteoclastogenesis and bone resorption through activating the Pi3K/akt signaling pathway via targeting Pten.

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Section: Expression Of Mir-21 Is Upregulated During Osteoclastogenesismentioning

confidence: 99%

miR‑21 promotes osteoclastogenesis through activation of PI3K/Akt signaling by targeting Pten in RAW264.7 cells

et al. 2020

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“…Thus, as an immune checkpoint, PD‐L1 has gained increasing attention for its significant role in the development, progression, and migration of tumors (Naidoo et al, 2016). PD‐L1, also known as the cluster of differentiation 274 (CD274) and B7 homolog 1 (B7‐H1), has been demonstrated to be upregulated in multiple tumor tissues (Koukourakis et al, 2018; Wan et al, 2018), including CRC (Dunne et al, 2016; Berntsson et al, 2018). MicroRNAs (miRNAs) are a group of endogenous gene‐encoded noncoding single RNAs of ~22 nucleotides in length (Anfossi et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‐93‐5p expression in tumor tissue and its tumor suppressor function via targeting programmed death ligand‐1 in colorectal cancer

Chen

Wang

Lin

et al. 2020

Cell Biology International

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This work aimed to investigate miR-93-5p expression in tumor tissue and its in vitro effects in colorectal cancer (CRC) by targeting programmed death ligand-1 (PD-L1). MiR-93-5p and PD-L1 expression was detected in CRC and adjacent normal tissues by quantitative real-time polymerase chain reaction and immunohistochemistry. The correlation between miR-93-5p and PD-L1 was validated by a dual-luciferase reporter assay. HCT116 and SW480 cells were divided into blank, miR-NC, miR-93-5p mimics, miR-93-5p inhibitor, PD-L1 small interfering RNA (siRNA) and miR-93-5p inhibitor + PD-L1 siRNA groups, and wound-healing and transwell assays were performed to detect cell migration and invasion, respectively. Protein expression was measured by western blotting. The secretion of cytokines was detected in the CRC cell/T coculture models. MiR-93-5p was downregulated in CRC tissues with upregulated PD-L1. In PD-L1-negative patients, miR-93-5p expression was increased compared with that in PD-L1-positive patients. MiR-93-5p and PD-L1 expression levels were associated with the tumor differentiation, lymphatic metastasis, TNM, Duke's stage, and prognosis of CRC. PD-L1 siRNA weakened the migration and invasion abilities via decreased expression of matrix metalloproteinase-1 (MMP-1), -2, and -9, and these effects were abolished by the miR-93-5p inhibitor. Additionally, anti-PD-L1 upregulated the expressions of interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), and interferon γ (IFN-γ) in the coculture of T cells with CRC cells, but downregulated the expressions of IL-1β, IL-10, and TGF-β. However, these changes were partially reversed by miR-93-5p inhibition. miR-93-5p is expected to be a novel target for CRC treatment since it decreases the migration and invasion, as well as the immune evasion, of CRC cells via targeting PD-L1. Abbreviations: CRC, colorectal cancer; ELISA, enzyme-linked immunosorbent assay; HE, hematoxylin and eosin; ITIM, immunoreceptor tyrosinebased inhibitory motif; miRNA, microRNA; PD-L1, programmed death ligand-1 Role of miR-93-5p in CRC via targeting PD-L1Y.-L. Chen et al.

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“…The data indicated that four miRNAs, miR-136-5p, miR-127-5p, miR-197 and miR-142-5p were closely associated with hsa_circ_0014130. Meanwhile, miR-136-5p, miR-127-5p, miR-197 and miR-142-5p have been found to play important roles in the progression of human cancers (4,(19)(20)(21). These data indicated that miR-136-5p, miR-127-5p, miR-197 and miR-142-5p might be the potential targets of hsa_circ_0014130 ( Fig.…”

Section: Hsa_circ_0014130 Functions As a Cerna Of Mir-136-5p In Nsclcmentioning

confidence: 79%

Circular RNA hsa_circ_0014130 Inhibits Apoptosis in Non–Small Cell Lung Cancer by Sponging miR-136-5p and Upregulating BCL2

Geng

Bao

Zhang

et al. 2020

Molecular Cancer Research

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Previous studies indicated that circular RNAs (circRNAs) played vital roles in the development of non-small-cell lung cancer (NSCLC). Although hsa_circ_0014130 might be a potential NSCLC biomarker, its function in NSCLC remains unknown. Thus, this study aimed to investigate the role of hsa_circ_0014130 in the progression of NSCLC. The levels of hsa_circ_0014130 in NSCLC tissues and adjacent normal tissues were determined by RT-qPCR. In addition, the expressions of Bcl-2, cleaved caspase 3 in A549 cells were detected with western blot. Meanwhile, the dual luciferase reporter system assay was used to determine the interaction of hsa_circ_0014130 and miR-136-5p, or Bcl-2 and miR-136-5p in NSCLC respectively. The level of hsa_circ_0014130 was significantly upregulated in NSCLC tissues. Downregulation of hsa_circ_0014130 markedly inhibited the proliferation and invasion of A549 cells via inducing apoptosis. In addition, downregulation of hsa_circ_0014130 inhibited the tumorigenesis of subcutaneous A549 xenograft in mice in vivo. Meanwhile, mechanistic analysis indicated that downregulation of hsa_circ_0014130 decreased the expression of miR-136-5p targeted gene Bcl-2 via acting as a competitive 'sponge' of miR-136-5p. In this study, we found that hsa_circ_0014130 was upregulated in NSCLC tissues. In addition, hsa_circ_0014130 functions as a tumor promoter in NSCLC to promote tumor growth through up-regulating Bcl-2 partially via 'sponging' miR-136-5p. Implications statementIn conclusion, hsa_circ_0014130 might function as a prognostic factor for patients with NSCLC and might be a therapeutic target for the treatment of NSCLC in future.

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miR-142-5p regulates CD4+ T cells in human non-small cell lung cancer through PD-L1 expression via the PTEN pathway

Cited by 35 publications

References 26 publications

miR‑21 promotes osteoclastogenesis through activation of PI3K/Akt signaling by targeting Pten in RAW264.7 cells

miR‑21 promotes osteoclastogenesis through activation of PI3K/Akt signaling by targeting Pten in RAW264.7 cells

MicroRNA‐93‐5p expression in tumor tissue and its tumor suppressor function via targeting programmed death ligand‐1 in colorectal cancer

Circular RNA hsa_circ_0014130 Inhibits Apoptosis in Non–Small Cell Lung Cancer by Sponging miR-136-5p and Upregulating BCL2

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