miR-20a regulates adipocyte differentiation by targeting lysine-specific demethylase 6b and transforming growth factor-β signaling

Zhou, Jie; Guo, Fei; Wang, G; Wang, J; Zheng, Fang; Guan, Xidong; Chang, Ailing; Zhang, X; Dai, Chunhua; Li, S; Li, X; Wang, B

doi:10.1038/ijo.2015.43

Cited by 38 publications

(28 citation statements)

References 37 publications

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“…All of these results indicated that SRCIN is a direct target of miR‐20a‐5p, while SRCIN and miR‐20a‐5p were negatively correlated in the pathogenesis of breast cancer. To be noted, miR‐20a has been reported to regulate adipocyte differentiation by targeting TGF‐β signaling, which is important growth factor in tumor microenvironment as earlier discussed, making miR‐20a‐5p as a more potential target for breast cancer therapy.…”

Section: Discussionmentioning

confidence: 99%

Breast cancer cell‐derived exosomal miR‐20a‐5p promotes the proliferation and differentiation of osteoclasts by targeting SRCIN1

Guo

Zhu

et al. 2019

Cancer Medicine

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Bone metastasis of breast cancer makes patients suffer from pain, fractures, spinal cord compression, and hypercalcemia, and is almost incurable. Although the mechanisms of bone metastasis in breast cancers have been studied intensively, novel specific target will be helpful to the development of new therapeutic strategy of breast cancer. Herein, we focused on the microRNA of tumor cell‐derived exosomes to investigate the communication between the bone microenvironment and tumor cells. The expression of miR‐20a‐5p in the primary murine bone marrow macrophages (BMMs), MCF‐10A, MCF‐7, and MDA‐MB‐231 cell lines, as well as the cell‐derived exosomes were assessed by qRT‐PCR. Transwell assays were used to evaluate the effects of miR‐20a‐5p on tumor cell migration and invasion. The expression of exosomes marker including CD63and TSG101 was detected by Western Blot. Cell cycle distribution of BMMs was analyzed by flow cytometry. 3‐UTR luciferase reporter assays were used to validate the putative binding between miR‐20a‐5p and SRCIN1. MiR‐20a‐5p was highly expressed in breast tumor tissues and the exosomes of MDA‐MB‐231 cells. MiR‐20a‐5p promoted migration and invasion in MDA‐MB‐231 cells, and the proliferation and differentiation of osteoclasts. MDA‐MB‐231 cell‐derived exosomes transferred miR‐20a‐5p to BMMs and facilitated the osteoclastogenesis via targeting SRCIN1. The present work provides evidence that miR‐20a‐5p transferred from breast cancer cell‐derived exosomes promotes the proliferation and differentiation of osteoclasts by targeting SRCIN1, providing scientific foundations for the development of exosome or miR‐20a‐5p targeted therapeutic intervention in breast cancer progression.

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Section: Discussionmentioning

confidence: 99%

Breast cancer cell‐derived exosomal miR‐20a‐5p promotes the proliferation and differentiation of osteoclasts by targeting SRCIN1

Guo

Zhu

et al. 2019

Cancer Medicine

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“…In addition, Zhang et al 41 verified that miR-181a promotes preadipocyte differentiation by inhibiting TGFBR1 expression in pigs, and Zhou et al 42 showed that miR-20a accelerates preadipocyte differentiation by suppressing Kdm6b and TGFBR2. 42 Bae et al 43 revealed that miR-291a-3p suppresses cellular senescence by targeting TGFBR2 in human dermal fibroblasts. 43 Smad2 and Smad3 were also shown to strongly influence cell proliferation and differentiation in chondrocytes in a study by Wang et al 44 Transfection with miR-375 mimics does not significantly affect the expression levels of Smad2/3 and Smad1/5/8 but dramatically reduces the levels of p-Smad2/3 and p-Smad1/5/8 in cumulus cells.…”

Section: Discussionmentioning

confidence: 99%

“…TGFBR1 directly binds Smad2 and Smad3 and then binds Smad4, forming a heteromeric F I G U R E 6 ssc-miR-204 inhibitor suppress preadipocyte apoptosis. 42 Bae et al 43 revealed that miR-291a-3p suppresses cellular senescence by targeting TGFBR2 in human dermal fibroblasts. B, Western blot analysis of the marker genes Bax and Bcl2.…”

Section: Discussionmentioning

confidence: 99%

ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2

Zhang

Wang

et al. 2019

J of Cellular Biochemistry

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MicroRNAs (miRNAs) take part in a variety of biological processes by regulating target genes. Transforming growth factor β receptor 1 (TGFBR1) and TGFBR2 are crucial members of the TGF‐β family and are serine/threonine kinase receptors. The aim of this study was to explore the functions of ssc‐miR‐204 in porcine preadipocyte differentiation and apoptosis with regard to the TGFβ/Smad pathway. We identified miRNAs predicted to target TGFBR1 and TGFBR2 using a database and selected ssc‐miR‐204 as a candidate miRNA. ssc‐miR‐204 overexpression dramatically reduced the levels of TGFBR1 and TGFBR2. However, after transfection with ssc‐miR‐204 inhibitor, TGFBR1 and TGFBR2 levels were dramatically increased. ssc‐miR‐204 overexpression dramatically promoted porcine preadipocyte differentiation and apoptosis. After transfection with ssc‐miR‐204 inhibitor, porcine preadipocyte differentiation and apoptosis were dramatically inhibited. After transfection with ssc‐miR‐204 mimics, Smad2, Smad3, Smad4, p‐Smad2, and p‐Smad3 protein levels significantly decreased, and adipogenesis was regulated by inhibiting the TGF‐β/Smad3 signaling pathway. Taken together, these results verified that ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2.

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“…In addition, miRNA families such as let-7, miR-30, miR-17, miR-148(Jing et al 2012) and miR-24(Qiang et al 2008; Kang et al 2013) are involved in animal adipose deposition. miR-20a regulates adipocyte differentiation by targeting lysine-specific demethylase 6 b and transforming growth cytokine β signal(Zhou et al). Previous studies(Michael et al 2009) assessed the anti-adipogenesis characteristics of miR-27b, which was down-regulated during adipocyte differentiation and weakened the induction of PPARc .…”

Section: Discussionmentioning

confidence: 99%

Screening and identification of MicroRNAs expressed in perirenal adipose tissue during rabbit growth

Wang

Guo

Tian

et al. 2019

Preprint

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45MiRNAs regulate adipose tissue development, which are closely 46 related to subcutaneous and intramuscular fat deposition and adipocyte 47 differentiation. As an important economic and agricultural animal, rabbits 48 have low adipose tissue deposition and are an ideal model to study 49 adipose regulation. However, the miRNAs related to fat deposition during 50 the growth and development of rabbits are poorly defined. In this study, 51 miRNA-sequencing and bioinformatics analyses were used to profile the 52 miRNAs in rabbit perirenal adipose tissue at 35, 85 and 120 days 53 post-birth. Differentially expressed (DE) miRNAs between different 54 stages were identified by DEseq in R. Target genes of DE miRNAs were 55 predicted by TargetScan and miRanda. To explore the functions of 56 identified miRNAs, Gene Ontology (GO) enrichment and Kyoto 57 Encyclopedia of Genes and Genomes (KEGG) pathway analyses were 58 performed. Approximately 1.6 GB of data was obtained by miRNA-seq. 59 A total of 987 miRNAs (780 known and 207 newly predicted) and 174 60 DE miRNAs were identified. The miRNAs ranged from 18nt to 26nt. GO 61 enrichment and KEGG pathway analyses revealed that the target genes of 62 the DE miRNAs were mainly involved in zinc ion binding, regulation of 63 cell growth, MAPK signaling pathway, and other adipose 64 hypertrophy-related pathways. Six DE miRNAs were randomly selected 65 and their expression profiles were validated by q-PCR. In summary, we 66 4 provide the first report of the miRNA profiles of rabbit adipose tissue 67 during different growth stages. Our data provide a theoretical reference 68 for subsequent studies on rabbit genetics, breeding and the regulatory 69 mechanisms of adipose development.70 Introduction 71 MicroRNAs (miRNAs) are endogenous non-coding RNAs, typically 72 18～26 nucleotides in length, that regulate gene expression in eukaryotic 73 cells. Mature miRNAs are produced from long primary transcripts 74 through a series of nucleases that are further assembled into 75 RNA-induced silencing complexes. These complexes recognize target 76 mRNAs by complementary base pairing, leading to mRNA degradation 77 and the inhibition of translation(Fabian et al. 2010). MiRNAs regulate a 78 wide range of physiological processes, including growth and 79 development, virus defense, cell proliferation, apoptosis and fat 80 metabolism. Meanwhile, it has been well documented that MiRNAs 81 regulate adipose tissue development, which are closely related to 82 subcutaneous and intramuscular fat deposition(Guoxi et al. 2011; Guo et 83 al. 2012) and adipocyte differentiation(Son et al. 2014).MiRNAs, 84 including miR-27b(Karbiener et al. 2009), miR-103(Meihang et al. 2015) 85 and miR-148a(Shi et al. 2015) regulate adipogenic processes, promoting 86 or inhibiting adipogenesis in animals. This implicates miRNAs can be a 87 5 new target for studying the molecular mechanisms governing fat 88 development, growth and deposition in animals. 89 To-date, studies on the role of miRNAs during fat development have 90 ...

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miR-20a regulates adipocyte differentiation by targeting lysine-specific demethylase 6b and transforming growth factor-β signaling

Abstract: The present work provides evidence that mouse miR-20a promotes adipocyte progenitor cells to differentiate and this function may depend upon its inhibitory effects on Kdm6b and TGF-β signaling.

Cited by 38 publications

References 37 publications

Breast cancer cell‐derived exosomal miR‐20a‐5p promotes the proliferation and differentiation of osteoclasts by targeting SRCIN1

Breast cancer cell‐derived exosomal miR‐20a‐5p promotes the proliferation and differentiation of osteoclasts by targeting SRCIN1

ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2

Screening and identification of MicroRNAs expressed in perirenal adipose tissue during rabbit growth

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