miR-21, miR-93, miR-191, miR-let-7b, and miR-499 Expression Level in Plasma and Cerebrospinal Fluid in Patients with Prolonged Disorders of Consciousness

Petrova, Tatiana A.; Kondratyev, S. A.; Kostareva, Anna; Savvina, I.A.; Kondratyeva, E.

doi:10.3390/neurolint15010004

Cited by 3 publications

(4 citation statements)

References 48 publications

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“…Although they focused on different miRNAs or employed different methodologies compared to our study, their findings are consistent in suggesting that miRNAs represent an intriguing target for evaluation in prolonged DoC. In patients with TBI, HIBI, or subarachnoid hemorrhage, Petrova and colleagues identified significant differences in the expression of miRNAs 93-5p, 21-5p, and 191-5p in both cerebrospinal fluid and plasma, depending on the specific etiologies 55 . Zilliox and colleagues examined the whole blood miRNA profile in six patients with post-traumatic DoC lasting an average of 1.5 years, discovering 48 miRNAs that were differentially expressed compared to controls 56 .…”

Section: Discussionsupporting

confidence: 80%

“…Two recent studies have investigated changes in miRNA expression in patients with prolonged DoC 55 , 56 . Although they focused on different miRNAs or employed different methodologies compared to our study, their findings are consistent in suggesting that miRNAs represent an intriguing target for evaluation in prolonged DoC.…”

Section: Discussionmentioning

confidence: 99%

“…One of the primary limitations is the small sample size, especially concerning HIBI and female patients, which may limit the generalizability of the results. Moreover, we evaluated miRNAs in the blood only, while analyzing miRNAs in the cerebrospinal fluid may possibly provide more accurate results regarding the changes occurring in the central nervous system 55 , 57 . Additionally, our assessment of outcomes was limited to a follow-up period of 6 months post-injury.…”

Section: Discussionmentioning

confidence: 99%

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Investigating microRNAs as biomarkers in disorders of consciousness: a longitudinal multicenter study

Musso,

Bivona,

Bonomo

et al. 2023

Sci Rep

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MicroRNAs (miRNAs) are involved in gene regulation and may affect secondary brain injury and recovery in patients with disorders of consciousness (DoC). This study investigated the role of five miRNAs (150-5p, 132-3p, 23b-3p, 451a, and 16-5p) in prolonged DoC. miRNA levels were assessed in serum samples from 30 patients with unresponsive wakefulness syndrome or minimally conscious state due to traumatic or hypoxic-ischemic brain injury (TBI, HIBI) at baseline (1–3 months) and 6 months post-injury. Patients’ diagnoses were determined using the Coma Recovery Scale revised, and functional outcomes were evaluated 6 months after injury with the Glasgow Outcome Scale Extended (GOSE) and the Functional Independence Measure (FIM). Compared to healthy controls, patients with TBI had lower levels of miRNAs 150-5p, 132-3p, and 23b-3p at baseline, while patients with HIBI had lower levels of miRNA 150-5p at baseline and 6 months post-injury and a reduction of miRNA 451a at baseline. Higher levels of miRNAs 132-3p and 23b-3p were associated with better outcomes in TBI patients as indicated by GOSE and FIM scores. This study highlights distinct miRNA dysregulated patterns in patients with prolonged DoC, dependent on etiology and post-injury time, and suggests that miRNAs 132-3p and 23b-3p may serve as prognostic biomarkers.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Investigating microRNAs as biomarkers in disorders of consciousness: a longitudinal multicenter study

Musso,

Bivona,

Bonomo

et al. 2023

Sci Rep

View full text Add to dashboard Cite

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“…Many studies have described the potential of miRNAs as biomarkers of the severity and outcome of COVID-19 [ 38 , 39 ]. In addition, miRNA profiling has also been investigated as biomarkers for neurological disease [ 40 , 41 , 42 ]. This study aimed to establish a relationship between miRNA and neurological manifestations in patients with COVID-19 and HHV-6, and to evaluate miRNAs as potential biomarkers of this pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

miRNAs in Neurological Manifestation in Patients Co-Infected with SARS-CoV-2 and Herpesvírus 6 (HHV-6)

Carneiro

Moreira

Coelho

et al. 2023

IJMS

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Human herpesviruses (HHVs) can establish latency and be reactivated, also are neurotropic viruses that can trigger neurological disorders. HHV-6 is a herpesvirus that is associated with neurological disorders. Studies have reported the detection of HHV-6 in patients with COVID-19 and neurological manifestations. However, specific diagnoses of the neurological disorders caused by these viruses tend to be invasive or difficult to interpret. This study aimed to establish a relationship between miRNA and neurological manifestations in patients co-infected with COVID-19 and HHV-6 and evaluate miRNAs as potential biomarkers. Serum samples from COVID-19 patients in the three cohorts were analyzed. miRNA analysis by real-time polymerase chain reaction (qPCR) revealed miRNAs associated with neuroinflammation were highly expressed in patients with neurological disorders and HHV-6 detection. When compared with the group of patients without detection of HHVs DNA and without neurological alterations, the group with detection of HHV-6 DNA and neurological alteration, displayed significant differences in the expression of mir-21, mir-146a, miR-155 and miR-let-7b (p < 0.01). Our results reinforce the involvement of miRNAs in neurological disorders and provide insights into their use as biomarkers for neurological disorders triggered by HHV-6. Furthermore, understanding the expression of miRNAs may contribute to therapeutic strategies.

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MicroRNA profiling of cerebrospinal fluid from dogs with steroid responsive meningitis-arteritis and meningoencephalitis of unknown origin

et al. 2023

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IntroductionNon-infectious inflammatory diseases of the central nervous system in dogs, such as steroid responsive meningitis-arteritis (SRMA) and meningoencephalitis of unknown origin (MUO), represent a common clinical challenge that needs extensive and multimodal work-up to reach a presumptive diagnosis. Both diseases are presumably caused by dysregulations of the immune system, but further research is needed in order to understand the molecular mechanisms behind each disease and to optimize treatment.MethodsBy next-generation sequencing and subsequent quantitative real-time PCR (qPCR) verification, we designed a prospective case–control pilot study to analyze the small RNA profiles of cerebrospinal fluid from dogs suffering from MUO (N = 5), dogs suffering from SRMA (N = 8), and healthy dogs (N = 5) presented for elective euthanasia used as the Control group.ResultsOur results showed an overall enrichment in Y-RNA fragments across all samples, followed by microRNAs (miRNAs) and ribosomal RNAs as the major findings. Additional traces of short RNA reads mapped to long non-coding RNAs and protein-coding genes were also found. From the detected canine miRNAs, miR-21, miR-486, miR-148a, miR-99a, miR-191 and miR-92a were among the most abundant. Dogs with SRMA showed higher differences in miRNA abundance than dogs with MUO when compared to healthy dogs, and miR-142-3p was consistently detected as differentially upregulated in both diseases, although at a low concentration. Moreover, miR-405-5p and miR-503-5p showed different profiles between SRMA and MUO dogs. Subsequent qPCR analyses confirmed miR-142-5p, miR-191-5p and miR-92a-3p as significantly upregulated miRNAs in dogs with SRMA and/or MUO.DiscussionCerebrospinal fluid is a challenging biological material to use for profiling miRNAs due to the low content of circulating RNAs. Despite this, we could confirm several miRNAs being differentially abundant when comparing healthy dogs and dogs with MUO and SRMA, respectively. The results of this study indicate a potential role of miRNAs in the underlying molecular mechanisms of these diseases and establish the basis for further studies.

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miR-21, miR-93, miR-191, miR-let-7b, and miR-499 Expression Level in Plasma and Cerebrospinal Fluid in Patients with Prolonged Disorders of Consciousness

Cited by 3 publications

References 48 publications

Investigating microRNAs as biomarkers in disorders of consciousness: a longitudinal multicenter study

Investigating microRNAs as biomarkers in disorders of consciousness: a longitudinal multicenter study

miRNAs in Neurological Manifestation in Patients Co-Infected with SARS-CoV-2 and Herpesvírus 6 (HHV-6)

MicroRNA profiling of cerebrospinal fluid from dogs with steroid responsive meningitis-arteritis and meningoencephalitis of unknown origin

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