MiR-21 overexpression in human primary squamous cell lung carcinoma is associated with poor patient prognosis

Gao, Wen; Shen, Hua; Liu, Lingxiang; Xu, Jian; Xu, Jing; Shu, Yongqian

doi:10.1007/s00432-010-0918-4

Cited by 219 publications

(168 citation statements)

References 38 publications

Supporting

Mentioning

154

Contrasting

Unclassified

Order By: Relevance

“…Treatment on Lovo cells by miR-143 inhibitor could irritate cell proliferation, while the overexpression of miR-143 could restrain cell proliferation. This study proved for the first time that miR-143 could restrain the growth of colon cancer cells by restraining the translation of K-RAS (Gao et al, 2011). Used miRNA chips to examine the non small-cell lung cancer (NSCLC) tissues and found 40 differently expressed miRNAs, where the expression of miR-451 was the lowest and was related to tumor differentiation, pathologic staging, and lymph node metastasis.…”

Section: Discussionmentioning

confidence: 96%

“…miRNA with diversified sequences, structure, abundance and expression ways can act as moderator in protein coding mRNA, and is important in gene expression, cell cycle regulation, and individual development. Recently, miRNA expression spectrum disorders were found in various tumors, including gastric cancer (Katada et al, 2009), nasopharyngeal carcinoma (Johnson et al, 2008), breast cancer (Hui et al, 2009), colon cancer (Chen et al, 2009) and lung cancer (Gao et al, 2011) indicating the important role of miRNA in regulation of tumor gene expressions. Because the gene regulation of miRNAs constitutes a powerful network, miRNAs show great potential: as a new treatment target and as a powerful intervention means.…”

Section: Discussionmentioning

confidence: 99%

“…According to phenotypic experiments, miR-451 inhibited invasive ability, induced cell cycle arrest in the G0/G1 phase, delayed the progression of cell cycle, inhibited cell proliferation and induced apoptosis in glioblastoma cells in vitro. It seems that miR-451 affects glioblastoma cells via regulation of the PI3K/AKT signaling pathway (Gao et al, 2011). Akt plays a critical role in the control of diverse cellular functions, such as protein synthesis, cell cycle, cell survival and apoptosis (Yang et al, 2004;Song et al, 2005;Chen et al, 2012), and may represent an important link between miR-451 and Bcl-2.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

MicroRNA-451 Inhibits Growth of Human Colorectal Carcinoma Cells via Downregulation of Pi3k/Akt Pathway

Zhang²,

Cai³

et al. 2013

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

MicroRNAs (MiRNAs) play important roles in coordinating a variety of cellular processes and abnormal expression has been linked to the occurrence of several cancers. The miRNA miR-451 is downregulated in colorectal carcinoma (CRC) cells, suggested by several research groups including our own. In this study, synthetic miR-451 mimics were transfected into the SW620 human CRC cell line using Lipofectamine 2000 and expression of miR-451 was analyzed by real time PCR, while expression of CAB39, LKB1, AMPK, AKT, PI3K and Bcl2 was analyzed by Western blot, and cell growth was detected by MTT assay. In comparison to the controls, a significant increase in the expression of miR-451 was associated with significantly decreased expression of CAB39, LKB1, AMPK, AKT, PI3K and Bcl2. The capacity of cell proliferation was significantly decreased by miR-451 expression, which also inhibited cell growth. Our study confirmed that miR-451 has a repressive role in CRC cells by inhibiting cell growth through down-regulating the P13K/AKT pathway.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

MicroRNA-451 Inhibits Growth of Human Colorectal Carcinoma Cells via Downregulation of Pi3k/Akt Pathway

Zhang²,

Cai³

et al. 2013

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

show abstract

“…The results suggested that low miR-21 expression was seen by adjuvant treatment in PDAC patients and anti-miR-21 increased anticancer drug activity in vitro. Gao et al [46] indicated that high-level miR-21 in squamous cell lung carcinoma tissue was associated with shortened survival time. On the contrary, Voortman and his colleagues [47] suggested that there was no significant association between the tested microRNAs (miR-21, miR-29b, miR-34a/b/c, miR-155 and let-7a) expression and survival as well as predicted chemotherapy response in resected NSCLC patients.…”

Section: Discussionmentioning

confidence: 99%

Reduction of plasma MicroRNA-21 is associated with chemotherapeutic response in patients with non-small cell lung cancer

Wei

Liu

Gao

et al. 2011

Chin. J. Cancer Res.

Self Cite

View full text Add to dashboard Cite

Objective: To examine plasma microRNA-21 (miR-21) level in patients with non-small cell lung cancer (NSCLC) and its potential correlation with chemotherapeutic response.Methods: 77 NSCLC patients and 36 age and sex-matched healthy controls were included. Plasma miR-21 concentration was examined using a quantitative real-time reverse transcription polymerase chain reaction assay (qRT-PCR). Potential correlation between plasma mir-21 concentrations with chemotherapeutic responses was analyzed in 35 patients with advanced NSCLC (stages IIIB and IV).Results: Plasma miR-21 was significantly higher in NSCLC patients relative to the healthy controls (P<0.0001). As a biomarker, plasma mir-21 had a receiver operating characteristic (ROC) curve area of 0.729 with 61.04% sensitivity and 83.33% specificity. Chemotherapeutic response in the 35 patients with advanced NSCLC (stages IIIB and IV) included partial response (PR) (n=11), stable disease and progression disease (SD+PD) (n=24). The overall response rate (CR+PR) was 31.4%. Plasma miR-21 in patients who achieved PR was significantly lower than those who did not respond (SD+PD) (P=0.0487), and comparable to that of the healthy controls (P=0.2744).Conclusion: Plasma miR-21 is a good biomarker for NSCLC, and could be used to predict responses to chemotherapy.

show abstract

“…miR-29 plays an indirect role in the inhibition of tumorigenicity through demethylation by DNA methyltransferases DNMT3A and -3B [49]. miR-126 has also been identified as an important inhibitory factor of vascular proliferation via the suppression of vascular epithelial growth factor (VEGF) [50]. Therefore miR-126 is identified as a tumor suppressor because it will be downregulated in cases of lung cancer [51].…”

Section: Tumor Suppressive Mirnas In Lung Cancermentioning

confidence: 99%

Effect of miRNAs in lung cancer suppression and oncogenesis

Zhang

Liu

2016

Open Life Sciences

View full text Add to dashboard Cite

MicroRNA (miRNAs) is a group of small noncoding RNAs. It is involved in multiple cellular processes including proliferation, development, metabolism, differentiation and apoptosis; many of which are linked to several pathological conditions, including cancer. Lung cancer is one of the leading causes of mortality in the world: in 2008 for example, there were 163,000 deaths as a result of lung cancer. Despite technologies emerging which provide the potential for novel targeted therapies and improved early diagnoses, the overall rate of fiveyear survival still remains at only 15%. One reason for this disappointing statistic is related to the presentation of the disease, and specifically a lack of markers for early detection. Notably, the expression of some miRNAs has been reported to be involved in the diagnosis, classification and even prognosis of lung cancer. Tumorsuppressive and oncogenic miRNAs were found in lung carcinogenesis and the biological functions of these miRNAs have been validated in transplantable lung cancer models and human paired normal-malignant lung tissue banks. Some of these tumor-suppressive and oncogenic miRNAs related to lung cancer will be reviewed here. This article will focus on emphasing miRNAs effectiveness as a biomarker in lung carcinogenesis and candidate pharmacology. Furthermore, how these findings improve our understanding of lung cancer biology and therapy will also be discussed.

show abstract

MiR-21 overexpression in human primary squamous cell lung carcinoma is associated with poor patient prognosis

Cited by 219 publications

References 38 publications

MicroRNA-451 Inhibits Growth of Human Colorectal Carcinoma Cells via Downregulation of Pi3k/Akt Pathway

MicroRNA-451 Inhibits Growth of Human Colorectal Carcinoma Cells via Downregulation of Pi3k/Akt Pathway

Reduction of plasma MicroRNA-21 is associated with chemotherapeutic response in patients with non-small cell lung cancer

Effect of miRNAs in lung cancer suppression and oncogenesis

Contact Info

Product

Resources

About