MiR-29 silencing modulates the expression of target genes related to proliferation, apoptosis and methylation in Burkitt lymphoma cells

Mazzoccoli, Luciano; Robaina, Marcela Cristina; Apa, Alexandre Gustavo; Bonamino, Martín; Pinto, Luciana Wernersbach; Queiroga, Eduardo; Bacchi, Carlos E.; Klumb, Claudete Esteves

doi:10.1007/s00432-017-2575-3

Cited by 38 publications

(35 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cell lines and treatments. The BL cell lines: Raji (EBV + ) obtained from the American Type Culture Collection (ATCC), Daudi (EBV + ) and BL41 (EBV -) kindly provided by Dr Boulanger (Greehey Children's Cancer Research Institute, The University of Texas Health Science Center, San Antonio, TX, USA), Namalwa (EBV + ) kindly provided by Dr Favaro (Laboratório de Biologia Molecular e Celular, Hemocentro, Unicamp, Campinas, São Paulo, Brazil) and Ramos (EBV -) provided by Professor Andrei Thomas-Tikhonenko (Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA) were cultured accordingly (21). Mycoplasma contamination PCR and short tandem repeat (STR) PCR were performed to confirm that the cell lines were mycoplasma-negative and to verify their genotypes.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

miR‑29 promoter and enhancer methylation identified by pyrosequencing in Burkitt lymhoma cells: Interplay between MYC and miR‑29 regulation

et al. 2019

Self Cite

View full text Add to dashboard Cite

Deregulation of microRNA expression plays a significant role in several cancer types including Burkitt lymphoma (BL). MicroRNA genes may be regulated through epigenetic mechanisms, such as specific histone modifications and/or DNA methylation of CpG islands in promoter regions, or by regions that are located next to microRNA genes. Given the regulatory role of MYC in miR-29 expression, methylation as an additional mechanism for miR-29 silencing was investigated. Methylation of miR-29a/b/c in BL tumour samples and BL cell lines (BL41 and Raji) was assessed by pyrosequencing assay. BL cells were treated with 5-aza-2'-deoxicitidine (decitabine) and evaluated for miR-29a/b/c expression and methylation status. MYC, DNMT1 and DNMT3B protein expression were accessed by western blotting. For Epstein-Barr virus (EBV) microRNA (miR)-BART6 inhibition, the cells were transiently transfected with anti-BART6-5p. BL tumour samples and BL cell lines presented miR-29a/b1 and miR-29b2/c genes methylated in CpG sites located in both the promoter and enhancer regions. The treatment of BL cells with decitabine reduced methylation, induced miR-29s expression and downregulated MYC protein levels in a dose-dependent manner. Notably, inhibition of EBV miR-BART6-5p combined with decitabine enhanced miR-29 expression in an EBV-BL cell line. In conclusion, the miR-29a/b1 and miR-29b2/c genes have methylated CpG sequences at promoter and enhancer regions that may contribute to the regulation of miR-29 expression in BL tumours. The present findings indicated interplay between MYC and miR-29 regulation, highlighting the potential role of EBV-miRNAs in miR-29 regulation for BL pathogenesis.

show abstract

Section: Methodsmentioning

confidence: 99%

“…no. 4464058) (Life Technologies; Thermo Fisher Scientific, Inc.) utilizing the method derived from Mazzoccoli et al (21). Then, the cells were treated with decitabine (1.0 µM) and after 72 h, the cells were harvested, and miRNA was extracted and quantified as aforementioned.…”

Section: Transfection Of An Inhibitor Of Ebv-mir-bart6-5pmentioning

confidence: 99%

miR‑29 promoter and enhancer methylation identified by pyrosequencing in Burkitt lymhoma cells: Interplay between MYC and miR‑29 regulation

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…These members are encoded by two gene clusters of miR-29b2/miR-29c (mapped on chromosome 1q32) and miR-29b1/miR-29a (mapped on chromosome 7q32). 42 Heterodimer-type (Class I) PI3Ks consists of a regulatory subunit that is at least encoded by three distinct genes (85α, p85β, p55γ) and a p110 catalytic subunit. 43 The p85α is the most frequently expressed regulatory isoform of PI3K, which encodes two minor alternative splicing isoforms of p55a and p50a additionally.…”

Section: Mir-29mentioning

confidence: 99%

<p>Major miRNA Involved in Insulin Secretion and Production in Beta-Cells</p>

Aghaei

Khodadadian

Karimi-Nazari

et al. 2020

IJGM

View full text Add to dashboard Cite

Insulin is implicated as a leading factor in glucose homeostasis and an important theme in diabetes mellitus (DM). Numerous proteins are involved in insulin signaling pathway and their dysregulation contributes to DM. microRNAs (miRNAs) as singlestrand molecules have a critical effect on gene expression at post-transcriptional levels. Intensive investigation done by DM researchers disclosed that miRNAs have a significant role in insulin secretion by direct targeting numerous proteins engaged in insulin signaling pathway; so, their dysregulation contributes to DM. In this review, we presented some major miRNAs engaged in the insulin production and secretion.

show abstract

“…Some mutations in the seed sequence of miR-142 generated novel potential binding sites of ZEB2, a transcriptional repressor associated with EBV proliferation, and some impaired the combination of miR-142 to its targets, including RAC1 and ADCY (129). (69,131,132). Promoter hypermethylation may also induce dysregulations of lncRNA, the lower expression of lncRNA CANDE in CLL, for example (65).…”

Section: Genetic Alterationsmentioning

confidence: 99%

The Role of Noncoding RNAs in B-Cell Lymphoma

Zou

Wan

et al. 2020

Front. Oncol.

View full text Add to dashboard Cite

In recent years, emerging evidence has suggested that noncoding RNAs (ncRNAs) participate in nearly every aspect of biological processes and play a crucial role in the genesis and progression of numerous tumors, including B-cell lymphoma. The exploration of ncRNA dysregulations and their functions in B-cell lymphoma provides new insights into lymphoma pathogenesis and is essential for indicating future clinical trials and optimizing the diagnostic and therapeutic strategies. In this review, we summarize the role of ncRNAs in B-cell lymphoma and discuss their potential in clinical applications.

show abstract

MiR-29 silencing modulates the expression of target genes related to proliferation, apoptosis and methylation in Burkitt lymphoma cells

Cited by 38 publications

References 69 publications

miR‑29 promoter and enhancer methylation identified by pyrosequencing in Burkitt lymhoma cells: Interplay between MYC and miR‑29 regulation

miR‑29 promoter and enhancer methylation identified by pyrosequencing in Burkitt lymhoma cells: Interplay between MYC and miR‑29 regulation

<p>Major miRNA Involved in Insulin Secretion and Production in Beta-Cells</p>

The Role of Noncoding RNAs in B-Cell Lymphoma

Contact Info

Product

Resources

About