miR-29c Suppresses the Malignant Phenotype of Hepatocellular Carcinoma Cells In Vitro by Mediating TPX2 Associated with Immune Infiltration

Wang, Haibo; Chen, Wanjin; Qi, Yong; Liu, Deng; Liu, Zhiqiang; Zhang, Qikun; Yi, Yujiao; Wang, Juanru; Wu, Wenyong

doi:10.1007/s10620-022-07810-3

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…A previous study reported that reduced TPX2 expression in HCC-infiltrating CD8 + T cells and downregulated TPX2 could restrict the anticancer effect of CD8 + T cells in HCC, whereas such antitumor activity could be enhanced by TPX2 overexpression (Wang et al 2022a). TPX2-associated immune infiltration participates in the inhibitory effect of miR-29c on the malignant phenotype of HCC cells (Wang et al 2023b). In colorectal cancer, TPX2 is closely associated with tumor-infiltrating lymphocytes (Guo et al 2023).…”

Section: Discussionmentioning

confidence: 99%

Integrated Bioinformatic Analysis Reveals the Oncogenic, Survival, and Prognostic Characteristics of TPX2 in Hepatocellular Carcinoma

Zhang,

Dong,

et al. 2024

Biochem Genet

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Targeting protein for Xenopus kinesin-like protein 2 (TPX2), a well-known mitotic protein, has been linked to carcinogenesis in several cancers. This study investigated the role of TPX2 in hepatocellular carcinoma (HCC) from various aspects using bioinformatic analyses. TPX2 expression and its prognostic value in pan-cancers were analyzed using SangerBox. TPX2 expression and its association with prognosis, immune infiltration, tumor mutations, and signaling pathways in HCC were analyzed using UALCAN, BoxKaplan-Meier Plotter, GEPIA, Human Protein Atlas, TIMER 2.0, and SangerBox. Genes co-expressed with TPX2 in HCC were analyzed using the HCCDB database, followed by functional enrichment using SangerBox. Clinical predictive models were established based on TPX2 and its co-expressed genes using the ACLBI database. TPX2 expression significantly increased in pan-cancers and was associated with survival in nearly half of the cancer types. High TPX2 expression has been linked to poor survival outcomes in patients with HCC. TPX2 expression was positively correlated with abundant infiltration of immune cells (including B cells, CD4 + /CD8 + T cells, macrophages, neutrophils, and dendritic cells), TP53 mutation, and carcinogenesis-related pathways, such as the PI3K/AKT/mTOR pathway, cellular response to hypoxia, and tumor proliferation signature. Nineteen genes were found to be co-expressed with TPX2 in HCC, and these genes showed close positive correlations and were mainly implicated in cell cycle-related functions. A prognostic model established using TPX2 and its expressed genes could stratify HCC patients into high- and low-risk groups, with a significantly shorter survival time in high-risk groups. The prognostic model performed well in predicting 1-, 3-, and 5-year survival of patients with HCC, with areas under the curve of 0.801, 0.725, and 0.711, respectively. TPX2 functions as an oncogene in HCC, and its high expression is detrimental to the survival of patients with HCC. Thus, TPX2 may be a prognostic biomarker and potential therapeutic target for HCC.

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Section: Discussionmentioning

confidence: 99%

Integrated Bioinformatic Analysis Reveals the Oncogenic, Survival, and Prognostic Characteristics of TPX2 in Hepatocellular Carcinoma

Zhang,

Dong,

et al. 2024

Biochem Genet

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E2F8—TPX2 axis regulates glycolysis and angiogenesis to promote progression and reduce chemosensitivity of liver cancer

Li,

Cao,

Deng

et al. 2024

Cytotechnology

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Identification of pyroptosis-associated miRNAs in the immunoscape and prognosis of hepatocellular carcinoma

Zheng,

Huang

2024

BMC Cancer

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Background Hepatocellular carcinoma is one of the most prevalent types of liver malignancy and poses a severe threat to global health. Despite recent improvements in therapeutic approaches, treatment options for patients with advanced or recurrent HCC are still limited. Materials and methods Our study analyzed miRNA differential expression using data from hepatocellular carcinoma patients in the Cancer Genome Atlas. Pyroptosis-related genes were identified from gene cards. Differential expression of miRNAs was analyzed using DESeq2 and visualized using ggplot2 and pheatmap. A prognostic risk model for pyroptosis-associated miRNAs was constructed using LASSO regression and validated by principal component analysis, Kaplan-Meier survival and ROC curve analysis. We also performed gene and pathway enrichment analysis. Immune cell infiltration and function in HCC were assessed using single-sample genomic enrichment analysis, and correlations with immune cells and function were explored. Also, CCK-8 assay as well as migration and invasion assays were performed after knockdown of miR-6844. Results We have established and validated a prognostic risk model based on ten DEmiRNAs, which is important for the survival of HCC patients. Significant changes in immune cell infiltration and immune function were also found in high-risk patients. It also demonstrated that knockdown of miR-6844 inhibited HCC cell proliferation, migration and invasion, highlighting its role in HCC progression. Conclusion Our study reveals the implications of pyroptosis-associated differential miRNAs on the prognosis of patients with hepatocellular carcinoma and provides a foundation for novel HCC therapies, especially immunotherapy. Supplementary Information The online version contains supplementary material available at 10.1186/s12885-024-13276-5.

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miR-29c Suppresses the Malignant Phenotype of Hepatocellular Carcinoma Cells In Vitro by Mediating TPX2 Associated with Immune Infiltration

Cited by 4 publications

References 32 publications

Integrated Bioinformatic Analysis Reveals the Oncogenic, Survival, and Prognostic Characteristics of TPX2 in Hepatocellular Carcinoma

Integrated Bioinformatic Analysis Reveals the Oncogenic, Survival, and Prognostic Characteristics of TPX2 in Hepatocellular Carcinoma

E2F8—TPX2 axis regulates glycolysis and angiogenesis to promote progression and reduce chemosensitivity of liver cancer

Identification of pyroptosis-associated miRNAs in the immunoscape and prognosis of hepatocellular carcinoma

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