miR‐30e‐5p suppresses cell proliferation and migration in bladder cancer through regulating metadherin

Zhang, Zhenxing; Qin, Haixiang; Jiang, Bo; Chen, Wei; Cao, Wenmin; Zhao, Xiaozhi; Yuan, Hui; Qi, Wei; Dong, Zuo-Ren; Guo, Hongqian

doi:10.1002/jcb.28866

Cited by 26 publications

(21 citation statements)

References 49 publications

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“…Studies have demonstrated that miRNAs has diagnostic and therapeutic values for many cancers [15][16][17]. MiR-30e-5p, which is a newly cancer-related miRNA, plays a pivotal role in the progression of different cancers, such as in non-small cell lung cancer [18], colorectal cancer [19], bladder cancer [20]. However, the functional role of miR-30e-5p in NPC is less reported.…”

Section: Introductionmentioning

confidence: 99%

MiR-30e-5p inhibits the migration and invasion of nasopharyngeal carcinoma via regulating the expression of MTA1

Yao²,

Li³

et al. 2020

Bioscience Reports

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The study explored the effect of miR-30e-5p on nasopharyngeal carcinoma (NPC). MiR-30e-5p levels in NPC cancer and adjacent normal samples, in metastatic and non-metastatic cancer samples of NPC, and in NP69 cell and five NPC cell lines were determined by quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between miR-30e-5p and MTA1 was confirmed by dual-luciferase reporter assay, Western blot and qRT-PCR. The viability, migration and invasion of 5-8F and 6-10B cells were determined by CCK-8, scratch test and transwell assays, respectively. The levels of migration-related proteins (vimentin and Snail) and invasion-related proteins (MMP2 and MMP3) in NPC cells were detected by Western blot. The results showed that low expression of miR-30e-5p was associated with HNSC cancer, NPC, metastasis of NPC and NPC cell lines. Overexpressed miR-30e-5p in HNSC cancer and NPC was predictive of a better prognosis of patients. In addition, the viability, migration and invasion were reduced by up-regulating miR-30e-5p in 5-8F cells, but promoted by down-regulated miR-30e-5p in 6-10B cells. MiR-30e-5p reversed the migration and invasion of NPC cells regulated by MTA1, and inhibited migration and invasion of NPC cells via regulating MTA1 expression.

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Section: Introductionmentioning

confidence: 99%

MiR-30e-5p inhibits the migration and invasion of nasopharyngeal carcinoma via regulating the expression of MTA1

Yao²,

Li³

et al. 2020

Bioscience Reports

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“…The clinical significance of miR‐30e‐5p, especially its prognostic value in SCCHN, was a key highlight of our current investigation. As a metastasis suppressor, miR‐30e‐5p has been reported to be downregulated in several malignant carcinomas, such as bladder cancer, colorectal cancer, nasopharyngeal carcinoma and head and neck cancer . In the available clinical studies based on tumor samples, low expression of miR‐30e‐5p always exists in patients with more aggressive clinical characteristics, including advanced TNM stages and metastasis, which is consistent with our conclusion.…”

Section: Discussionsupporting

confidence: 90%

“…Consistent with the clinical results, loss and gain of functional experiments clearly indicated the critical role of miR‐30e‐5p in SCCHN metastasis. Several studies have confirmed that miR‐30e‐5p is a microRNA that is closely associated with metastasis in vitro; however, how miR‐30e‐5p blocks cancer metastasis and its exact molecular mechanisms remain largely unknown. In the process of metastasis, metastatic cancer cells are required to detach the primary lesion and enhance the migratory and invasive abilities, in which EMT is indispensable in the initiation of metastasis .…”

Section: Discussionmentioning

confidence: 99%

miR‐30e‐5p represses angiogenesis and metastasis by directly targeting AEG‐1 in squamous cell carcinoma of the head and neck

Zhang

Liu

et al. 2019

Cancer Science

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Metastasis is a critical determinant for the treatment strategy and prognosis in patients with squamous cell carcinoma of the head and neck (SCCHN). However, the mechanisms underlying SCCHN metastasis are poorly understood. Our study sought to determine the key microRNA and their functional mechanisms involved in SCCHN metastasis. For The Cancer Genome Atlas (TCGA) data analysis, quantitative PCR was used to quantify the level of miR-30e-5p in SCCHN and its clinical significance was further analyzed. A series of in vitro and in vivo experiments were applied to determine the effects of miR-30e-5p and its target AEG-1 on SCCHN metastasis. A mechanism investigation further revealed that AEG-1 was implicated in the angiogenesis and metastasis mediated by miR-30e-5p. Overall, our study confirms that miR-30e-5p is a valuable predictive biomarker and potential therapeutic target in SCCHN metastasis. K E Y W O R D SAEG-1, angiogenesis, metastasis, miR-30e-5p, squamous cell carcinoma of the head and neck

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“…MicroRNAs (miRNAs) are small non-coding RNA molecules that bind to the 3'-untranslated region (3'UTR) of corresponding messenger RNAs (mRNAs). MiRNAs have been reported to have a potential role as diagnostic biomarkers in ovarian cancer [13], colorectal cancer [14], and pancreatic cancer [15]. In 2016, Zhou et al identified a six-miRNA diagnostic panel that were upregulated in the plasma of patients with adenocarcinoma of the lung in an Asian population [16].…”

Section: Introductionmentioning

confidence: 99%

Upregulation of the Coatomer Protein Complex Subunit beta 2 (COPB2) Gene Targets microRNA-335-3p in NCI-H1975 Lung Adenocarcinoma Cells to Promote Cell Proliferation and Migration

Jiang

et al. 2020

Med Sci Monit

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Departmental sources Background: The coatomer protein complex subunit beta 2 (COPB2) gene is upregulated and promotes cell proliferation in some cancer cells. This study aimed to investigate the role of microRNA (miRNA) targeting by COPB2 gene expression in human lung adenocarcinoma cell lines, including NCI-H1975 cells. Material/Methods: COPB2 expression in normal human bronchial epithelial cells and lung adenocarcinoma cells was measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot. NCI-H1975 human lung adenocarcinoma cells were transfected with short-interfering COPB2 (siCOPB2). Cell apoptosis and cell proliferation were evaluated by flow cytometry and Cell Counting Kit-8 (CCK-8) assays, respectively. The transwell assay evaluated cell migration. Targeting of miR-335-3p by COPB2 was predicted using TargetScan 7.2 and verified using a dual-luciferase reporter assay in NCI-H1975 cells. MiR-335-3p mimics were transfected into NCI-H1975 cells. The further functional analysis included detection of protein expression for cyclin D1, tissue inhibitor matrix metalloproteinase-1 (TIMP-1), matrix metallopeptidase 9 (MMP9), Bcl-2, and Bax, to verify the role of miR-335-3p targeting by COPB2 in lung adenocarcinoma cells. Results: COPB2 was upregulated in lung adenocarcinoma cells and was a direct target of miR-335-3p mimics. COPB2 knockdown promoted cell apoptosis, inhibited cell migration and proliferation in NCI-H1975 cells. The effects of COPB2 knockdown on NCI-H1975 cells were increased by miR-335-3p mimics, which also further reduced the expression levels of cyclin D1, MMP9, and Bcl-2 and further increased TIMP-1 and Bax by siCOPB2. Conclusions: This study showed that COPB2 was the functional target of miR-335-3p in NCI-H1975 human adenocarcinoma cells.

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miR‐30e‐5p suppresses cell proliferation and migration in bladder cancer through regulating metadherin

Cited by 26 publications

References 49 publications

MiR-30e-5p inhibits the migration and invasion of nasopharyngeal carcinoma via regulating the expression of MTA1

MiR-30e-5p inhibits the migration and invasion of nasopharyngeal carcinoma via regulating the expression of MTA1

miR‐30e‐5p represses angiogenesis and metastasis by directly targeting AEG‐1 in squamous cell carcinoma of the head and neck

Upregulation of the Coatomer Protein Complex Subunit beta 2 (COPB2) Gene Targets microRNA-335-3p in NCI-H1975 Lung Adenocarcinoma Cells to Promote Cell Proliferation and Migration

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