miR-320a regulates cell proliferation and apoptosis in multiple myeloma by targeting pre-B-cell leukemia transcription factor 3

Lu, Yinghao; Wu, Depei; Wang, Jishi; Li, Yan; Chai, Xiangping; Kang, Qian

doi:10.1016/j.bbrc.2016.04.069

Cited by 40 publications

(37 citation statements)

References 29 publications

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“…In addition, the emergence of next-generation sequencing technology has made the detection of serum miRNAs more convenient and accurate (Wang et al 2010;Lu et al 2016;Amodio et al 2016). However, there is currently a lack of studies on serum miRNAs in AD.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have also reported that the levels of miRNAs, including miR-27a, 9, 137, 181c, 146a, 155, 424, and 29c, are altered in the cerebrospinal fluid of AD patients (Geekiyanage and Chan 2011;Alexandrov et al 2012;Sala Frigerio et al 2013). Recent studies have revealed that the serum levels of miRNAs are stable and that miRNAs can be used to diagnose conditions, such as acute myocardial infarction and multiple myeloma (Wang et al 2010;Lu et al 2016;Amodio et al 2016). In addition, an increasing number of studies have analyzed serum miRNAs in patients with nervous system-related disorders.…”

Section: Introductionmentioning

confidence: 99%

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Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

et al. 2017

Tohoku J. Exp. Med.

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MicroRNAs (miRNAs) are short noncoding RNA that participate in posttranscriptional gene regulation. However, little is understood about the roles of miRNAs in Alzheimer's disease (AD). In this study, we used next-generation sequencing on RNA extracted from the serum samples of 20 AD patients and 20 controls, yielding a total of 72 miRNAs with significantly changed expression levels. Among these candidates, we selected 9 miRNAs with most significant alteration in disease, and validated their expression levels using RT-qPCR analysis on serum samples from 45 AD patients and 40 control subjects. Thus, the serum levels of miR-146a-5p, 106b-3p, 195-5p, 20b-5p, and 497-5p were significantly higher, while those of miR-125b-3p, 29c-3p, 93-5p and 19b-3p were significantly lower in AD patients, compared with control subjects. Two miRNAs, miR-29c-3p and miR-19b-3p, were selected because both RNA deep-sequencing and q-PCR methods indicated lower serum levels of these miRNAs in AD patients. Computational analysis predicted that 3′-untranslated region of signal transduction and activator of transcription 3 (STAT3) mRNA is targeted both by miR-29c-3p and miR-19b-3p. Using SH-SY5Y human neuroblastoma cells, we showed that transfection with miR-29c-3p or miR-19b-3p inhibitor significantly increased STAT3 phosphorylation. Furthermore, Water maze test, which assesses the learning and memory deficits in rodents, showed that escape latency was significantly shorter in AD rats with overexpression of miR-29c-3p or miR-19b-3p than in control AD rats. These results suggest that miR-29c-3p or miR-19b-3p may contribute to the cognitive function. In conclusion, the serum levels of miR-29c-3p and miR-19b-3p are helpful biomarkers for AD.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

et al. 2017

Tohoku J. Exp. Med.

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“…Additionally, miR-320a has been observed to be an independent prognostic factor where decreased miR-320a expression is correlated with lower survival in invasive breast cancer. [32] The anti-proliferative and tumor-suppressing effects of miR-320a have also been recorded in lung cancer [33], prostate cancer [34], gastric cancer [35], leukemia [36, 37], and nasopharyngeal carcinoma [38]. …”

Section: Discussionmentioning

confidence: 99%

Identification of MicroRNAs as Breast Cancer Prognosis Markers through the Cancer Genome Atlas

et al. 2016

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Breast cancer is the second-most common cancer and second-leading cause of cancer mortality in American women. The dysregulation of microRNAs (miRNAs) plays a key role in almost all cancers, including breast cancer. We comprehensively analyzed miRNA expression, global gene expression, and patient survival from the Cancer Genomes Atlas (TCGA) to identify clinically relevant miRNAs and their potential gene targets in breast tumors. In our analysis, we found that increased expression of 12 mature miRNAs—hsa-miR-320a, hsa-miR-361-5p, hsa-miR-103a-3p, hsa-miR-21-5p, hsa-miR-374b-5p, hsa-miR-140-3p, hsa-miR-25-3p, hsa-miR-651-5p, hsa-miR-200c-3p, hsa-miR-30a-5p, hsa-miR-30c-5p, and hsa-let-7i-5p —each predicted improved breast cancer survival. Of the 12 miRNAs, miR-320a, miR-361-5p, miR-21-5p, miR-103a-3p were selected for further analysis. By correlating global gene expression with miRNA expression and then employing miRNA target prediction analysis, we suggest that the four miRNAs may exert protective phenotypes by targeting breast oncogenes that contribute to patient survival. We propose that miR-320a targets the survival-associated genes RAD51, RRP1B, and TDG; miR-361-5p targets ARCN1; and miR-21-5p targets MSH2, RMND5A, STAG2, and UBE2D3. The results of our stringent bioinformatics approach for identifying clinically relevant miRNAs and their targets indicate that miR-320a, miR-361-5p, and miR-21-5p may contribute to breast cancer survival.

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“…This miRNA has an inhibitory effect on proliferation and motility and is involved in the induction of apoptosis (18). It has been found to be down regulated in breast, colorectal tumours, hepatocellular carcinomas (13) and multiple myeloma (18).…”

Section: Exosomes and The Tsg Mirna-230amentioning

confidence: 99%

“…It has been found to be down regulated in breast, colorectal tumours, hepatocellular carcinomas (13) and multiple myeloma (18). So far two targets genes have been identified: cMyc, whose role as oncogene needs no introduction and PBX3 (19) which belong to the Pre-B-cell leukemia homeobox family PBX.…”

Section: Exosomes and The Tsg Mirna-230amentioning

confidence: 99%

Exosomes: recruits for tumour surveillance?

Pezzella

2017

J. Thorac. Dis.

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miR-320a regulates cell proliferation and apoptosis in multiple myeloma by targeting pre-B-cell leukemia transcription factor 3

Cited by 40 publications

References 29 publications

Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

Identification of MicroRNAs as Breast Cancer Prognosis Markers through the Cancer Genome Atlas

Exosomes: recruits for tumour surveillance?

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