MiR-325-3p functions as a suppressor miRNA and inhibits the proliferation and metastasis of glioma through targeting FOXM1

Abstract

Glioma is a malignant brain tumor exhibiting high levels of proliferation and metastasis, and these have been related to its poor prognosis and high mortality rate. MicroRNA (miRNA)-325-3p exhibits tissue-specific expression profiles and is aberrantly expressed in multiple types of malignant tumors. Our research focuses on determining the function and mechanism of action of miR-325-3p in glioma. The relative expression levels of miR-325-3p in glioma tumor tissues and cell lines were verified by qRT-PCR. The effect of 325-3p on glioma tumor cell behavior was assessed using CCK-8 assays, EDU staining, colony formation assays, flow cytometry, transwell invasion assays, and a xenograft model. In addition, we searched for miR-325-3p targets, and their potential mechanism of action was demonstrated using a reporter assay and rescue experiments. Results showed that the expression levels of miR-325-3p in glioma cancer tissues and tumor cell lines were significantly lower than that of normal paired adjacent tissue or normal cell lines. Functional experiments illustrated that tumor proliferation, migration and invasion were suppressed via upregulation of miR-325-3p. To assess whether FOXM1 is a target of miR-325-3p, we examined this hypothesis using a luciferase report assay and then found that miR-325-3p could modulate the expression of FOXM1. Furthermore, the functional role of miR-325-3p was also confirmed in a xenograft model using nude mice. Together, our data demonstrated that in glioma, miR-325-3p may inhibit cancer cell growth through the suppression of FOXM1 and could be a promising new target for treating this type of brain cancer.

Long non-coding RNA (FALEC) promotes malignant behaviors of gastric cancer cells by regulating miR-203b/PIM3 axis

“…Research also shows that miRNAs can directly degrade mRNA or prevent translation by negatively regulating target genes and affecting a number of cell activities, such as cell growth, proliferation, apoptosis, autophagy, inflammation, invasion, and metastasis (36,42,43). Additionally, we also predicted the potential target genes of miR-203b using TargetScan, and PIM3 was identified to be the potential target gene of miR-203b.…”

“…The hypothesis of ceRNA is a common mechanism of lncRNA in regulating tumor progression (34). MiRNAs have functions similar to oncogenes or tumor suppressor genes and are closely related to the development process of human tumors (35,36).…”

Hsa_circ_0002980 prevents proliferation, migration, invasion, and epithelial-mesenchymal transition of liver cancer cells through microRNA-1303/cell adhesion molecule 2 axis

“…In cancer, a key mechanism by which circRNAs exert their function is by targeting binding to some tumorassociated miRNAs and suppressing the function of these miRNAs [26]. MiRNAs, as a group of singlestranded RNAs, were found to have a crucial role in tumor progression, especially in LC [27,28]. Multiple studies demonstrated that miRNAs can in uence LC progression by regulating cell proliferation, apoptosis, metastasis and immune escape [28,29].…”

Cancer‐associated fibroblast‐derived exosomal microRNA‐20a suppresses the PTEN/PI3K‐AKT pathway to promote the progression and chemoresistance of non‐small cell lung cancer

“…19,20 miRNAs are a kind of short non-coding RNAs that suppress target mRNA at the posttranscriptional level through binding to the complementary sequences within their 3′-UTR sequences and destabilizing these mRNAs or preventing their translation. 21,22 Recent research suggests that TAMderived exosomes can transfer miR-21 to gastric carcinoma cells, thereby conferring resistance to cisplatin (DDP). 23 CAFs created from patient-derived pancreatic cancer tumour samples were innately chemoresistant and played positive roles in promoting multiplication and chemotherapy resistance of pancreatic carcinoma cells through exosome signalling.…”

“…Some reports have highlighted key functional roles for exosomes in the context of lung cancer development 19,20 . miRNAs are a kind of short non‐coding RNAs that suppress target mRNA at the posttranscriptional level through binding to the complementary sequences within their 3′‐ UTR sequences and destabilizing these mRNAs or preventing their translation 21,22 . Recent research suggests that TAM‐derived exosomes can transfer miR‐21 to gastric carcinoma cells, thereby conferring resistance to cisplatin (DDP) 23 .…”