miR-451a was selectively sorted into exosomes and promoted the progression of esophageal squamous cell carcinoma through CAB39

Abstract

Exosomes are emerging mediators of cell-cell communication, which are secreted from cells and may be conveyed to recipient cells for cell biological processes. Here, we examined exosome miRNA expression in esophageal squamous cell carcinoma (ESCC) cells. We examine different miRNA expressions in cells and exosomes. To study the mechanisms of miRNA packaging into exosomes, we combined exosome proteomic data which is miR-451a interacts with YWHAE. Esophageal squamous tissues and matched tissues were compared from 155 patients, and CAB39 is related to TGF-β1. We found that miR-451a was encapsulated in the exosomes. Overexpression of YWHAE leads to miR-451a accumulation in the exosomes instead of donor cells. Furthermore, CAB39 was targeted with miR-451a. We found that CAB39 weakens antitumor immunity through TGF-β1 in ESCC. In summary, our data demonstrated that YWHAE selectively sorted miR-451a into exosomes and through the target of CAB39 weakened antitumor immunity promotes tumor progression.