MiR-7-5p attenuates vascular smooth muscle cell migration and intimal hyperplasia after vascular injury by NF-kB signaling

Yuan, Jixiang; Kong, Yun

doi:10.1016/j.bbrep.2022.101394

Cited by 2 publications

(2 citation statements)

References 35 publications

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“…Most studies in animal models of cerebral ischemia and intracerebral hemorrhage have indicated a decrease in miR-7–5P levels in brain tissue samples ( 33 – 36 ). Similarly, in a model of carotid artery injury, miR-7–5p was found to be downregulated when examining carotid endarterectomy samples( 37 ). Similar to 140–5p, the decreases in tissue miR-7–5p levels might be attributed to the release of miR-7–5P from injured tissue into the serum.…”

Section: Resultsmentioning

confidence: 97%

MicroRNA Expression Profile in Acute Ischemic Stroke

Mainali,

Nepal,

Webb

et al. 2024

Preprint

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Introduction: Acute ischemic stroke with large vessel occlusion (LVO) continues to present a considerable challenge to global health, marked by substantial morbidity and mortality rates. Although definitive diagnostic markers exist in the form of neuroimaging, their expense, limited availability, and potential for diagnostic delay can often result in missed opportunities for life-saving interventions. Despite several past attempts, research efforts to date have been fraught with challenges likely due to multiple factors such as inclusion of diverse stroke types, variable onset intervals, differing pathobiologies, and a range of infarct sizes, all contributing to inconsistent circulating biomarker levels. In this context, microRNAs (miRNAs) have emerged as a promising biomarker, demonstrating potential as biomarkers across various diseases, including cancer, cardiovascular conditions, and neurological disorders. These circulating miRNAs embody a wide spectrum of pathophysiological processes, encompassing cell death, inflammation, angiogenesis, neuroprotection, brain plasticity, and blood-brain barrier integrity. This pilot study explores the utility of circulating exosome-enriched extracellular vesicle (EV) miRNAs as potential biomarkers for anterior circulation LVO (acLVO) stroke.Methods: In our longitudinal prospective cohort study, we collected data from acute large vessel occlusion (acLVO) stroke patients at four critical time intervals post-symptom onset: 0–6 hours, 6–12 hours, 12–24 hours, and 5–7 days. For comparative analysis, healthy individuals were included as control subjects. In this study, extracellular vesicles (EVs) were isolated from the plasma of participants, and the miRNAs within these EVs were profiled utilizing the NanoString nCounter system. Complementing this, a scoping review was conducted to examine the roles of specific miRNAs such as miR-140-5p, miR-210-3p, and miR-7-5p in acute ischemic stroke (AIS). This review involved a targeted PubMed search to assess their influence on crucial pathophysiological pathways in AIS, and their potential applications in diagnosis, treatment, and prognosis. The review also included an assessment of additional miRNAs linked to stroke.Results: Within the first 6 hours of symptom onset, three specific miRNAs (miR-7-5p, miR-140-5p, and miR-210-3p) exhibited significant differential expression compared to other time points and healthy controls. These miRNAs have previously been associated with neuroprotection, cellular stress responses, and tissue damage, suggesting their potential as early markers of acute ischemic stroke.Conclusion: This study highlights the potential of circulating miRNAs as blood-based biomarkers for hyperacute acLVO ischemic stroke. However, further validation in a larger, risk-matched cohort is required. Additionally, investigations are needed to assess the prognostic relevance of these miRNAs by linking their expression profiles with radiological and functional outcomes.

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Section: Resultsmentioning

confidence: 97%

MicroRNA Expression Profile in Acute Ischemic Stroke

Mainali,

Nepal,

Webb

et al. 2024

Preprint

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“…The important role of p38MAPK in proliferation and migration of vascular SMC was observed in vitro study ( 70 ). In addition, NF-κB is also involved in migration of SMC ( 71 , 72 ). Therefore, inhibiting proliferation and migration of SMC by downregulating the p38MAPK/NF-κB signaling pathway is another possible mechanism for the anti-atherogenesis effect of MO.…”

Section: Discussionmentioning

confidence: 99%

Mussel oil is superior to fish oil in preventing atherosclerosis of ApoE−/− mice

Li,

Song,

et al. 2024

Front. Nutr.

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ObjectivesThe present study aimed to explore the preventive effect of mussel oil (MO) on atherosclerosis and the potential mechanism in apolipoprotein E-null (ApoE−/−) mice.MethodsApoE−/− mice were fed with a high-fat and high-cholesterol chow and given corn oil (CO), fish oil (FO), MO, or aspirin (ASP, dissolved in CO) by gavage for 12 weeks. The total n-3 polyunsaturated fatty acids (PUFAs) in MO (51.01%) and FO (46.82%) were comparable (mainly C22:6n-3 and C20:5n-3). Wild-type mice were fed with a normal chow and given equivalent CO as health control (CON).ResultsCompared with the CON group, obvious atherosclerotic plaque appeared at aorta and aortic sinus in the CO group. Compared with the CO group, MO but not FO had a significantly smaller atherosclerotic plaque area in the aorta. The aortic atherosclerotic plaque area was comparable in the MO, CON, and ASP groups. The MO group had a significantly smaller atherosclerotic plaque area, lower lipid deposition, lower contents of smooth muscle cell (SMC), and slightly lower contents of macrophage at the aortic sinus than the FO group. Serum concentrations of IL-1β, NF-κB, and VCAM-1 were comparable in the MO and FO groups and were significantly lower than the CO group. Compared with the CO group, the MO group but not FO group had significantly lower aortic protein levels of p65NF-κB, p38MAPK, and VCAM-1. The aortic protein levels of p-p65NF-κB and p-p38MAPK were significantly lower in the MO group than the FO group.ConclusionIn conclusion, MO is more potent than FO in preventing atherosclerosis, and the possible mechanism may be by downregulating p38MAPK/NF-κB signaling pathway, decreasing VCAM-1 and macrophage, and inhibiting proliferation and migration of SMC.

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MiR-7-5p attenuates vascular smooth muscle cell migration and intimal hyperplasia after vascular injury by NF-kB signaling

Cited by 2 publications

References 35 publications

MicroRNA Expression Profile in Acute Ischemic Stroke

MicroRNA Expression Profile in Acute Ischemic Stroke

Mussel oil is superior to fish oil in preventing atherosclerosis of ApoE−/− mice

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