miR-96 functions as a tumor suppressor gene by targeting NUAK1 in pancreatic cancer

Huang, Xuan; Lv, Wei; Zhang, Jianhua; Lu, Da‐Lin

doi:10.3892/ijmm.2014.1940

Cited by 52 publications

(43 citation statements)

References 30 publications

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“…However, some studies suggested that miR‐96 might play an unusual role in pancreatic cancer. According to the studies of Yu et al and Huang et al, down‐regulation of miR‐96 was found in pancreatic cancer and miR‐96 overexpression inhibited cell proliferation and migration . In our study, we verified the low expression of miR‐96 and its tumor‐suppressive function in pancreatic cancer.…”

Section: Discussionsupporting

confidence: 79%

Retracted: LncRNA UCA1 impacts cell proliferation, invasion, and migration of pancreatic cancer through regulating miR‐96/FOXO3

et al. 2018

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This study was expected to reveal the regulatory effects of lncRNA UCA1 on pancreatic cancer cell progression through targeting miR-96/FOXO3. Microarray analysis was carried out on 36 cases of pancreatic cancer tissues and 16 cases of adjacent tissues among them. Expression levels of lncRNA UCA1, miR-96, and FOXO3 in pancreatic cancer tissues and cell lines were determined by qRT-PCR. Expression levels of FOXO3 protein were determined by western blot. Cell viability, cell cycle and apoptosis, cell invasion and migration were detected by CCK-8, flow cytometry, and transwell assay, respectively. The colocalization relationship between lncRNA UCA1 and miR-96 was detected by RNA FISH. Whether UCA1 could target miR-96 and whether miR-96 could target FOXO3 3'UTR were verified by dual-luciferase reporter gene assay. High expression of lncRNA UCA1 and FOXO3 and low expression of miR-96 were shown in pancreatic cancer. Inhibition of UCA1 suppressed pancreatic tumor cell proliferation, colony formation, and metastasis, while inhibition of miR-96 promoted pancreatic cancer cell progression. FOXO3 was the downstream target gene of miR-96 and showed the opposite effects. LncRNA UCA1 promoted cell proliferation, invasion, migration and inhibited cell apoptosis of pancreatic cancer through down-regulating miR-96 and up-regulating FOXO3. © 2018 IUBMB Life, 70(4):276-290, 2018.

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Section: Discussionsupporting

confidence: 79%

Retracted: LncRNA UCA1 impacts cell proliferation, invasion, and migration of pancreatic cancer through regulating miR‐96/FOXO3

et al. 2018

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“…Many of the differentially expressed protein-coding genes play roles in carcinogenesis (e.g. GAGE12J, VWA2, NUAK1, and MGAT4C), consistent with the transcriptional differences we identified between the NTF2 low and NTF2 high dox+ cell lines (Chen et al, 2013;Demichelis et al, 2012;Gonzalez et al, 2018;Huang et al, 2014).…”

Section: Identification Of Differentially Expressed Genes In Cells Exsupporting

confidence: 83%

Nuclear Transport Factor 2 (NTF2) suppresses metastatic melanoma by modifying cell migration, metastasis, and gene expression

Vuković

White

Gigley

et al. 2020

Preprint

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While changes in nuclear structure and organization are frequently observed in cancer cells, relatively little is known about how nuclear architecture impacts cancer progression and pathology. To begin to address this question, we studied Nuclear Transport Factor 2 (NTF2) because its levels decrease during melanoma progression.We show that increasing NTF2 expression in metastatic melanoma cells reduces cell proliferation and motility while increasing apoptosis. We also demonstrate that increasing NTF2 expression in these cells significantly inhibits metastasis and increases survival of mice. Mechanistically, we show that NTF2 levels affect the expression and nuclear positioning of a number of genes associated with cell proliferation and migration. We propose that by decreasing nuclear size and/or lamin A nuclear localization, ectopic expression of NTF2 in metastatic melanoma alters chromatin organization to generate a gene expression profile with characteristics of primary melanoma, concomitantly abrogating several phenotypes associated with advanced stage cancer both in vitro and in vivo. Thus NTF2 acts as a melanoma tumor suppressor to maintain proper nuclear structure and gene expression and could be a novel therapeutic target to improve health outcomes of melanoma patients.

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“…In the majority of the tumors, miR-96 acts as an oncogene to promote the proliferation and invasion of cancer cells by inhibiting transcription factor FOXO1 (24), FOXO3a (25), tumor suppressor protein RECK (26) and metastasis suppressor protein MTSS1 (27). However, in pancreatic cancer, miR-96 functions as a tumor suppressor by targeting HERG1 and NUAK1 (28,29).…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of miR-96, a potential biomarker of NSCLC, through bioinformatic analysis

et al. 2017

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Lung cancer is the leading cause of cancer-related death worldwide. The poor prognosis is partly due to lack of efficient methods for early diagnosis. MicroRNAs play roles in almost all aspects of cancer biology, and can be secreted into the circulation and serve as molecular biomarkers for the early diagnosis of cancer. In the present study, we determined the expression of miR-96 and the function of its target genes in lung cancer through bioinformatic analysis. Four microRNA expression profiles of lung cancer were downloaded from Gene Expression Omnibus and the data were analyzed using SPSS 16.0 software. Compared to the control group, expression of miR-96 was significantly increased in non-small cell lung cancer (NSCLC) (GSE51855), lung adenocarcinoma (GSE48414), stage I adenocarcinoma tissues (GSE63805) and the plasma of lung cancer patients (GSE68951). miR-96 was also elevated in six different NSCLC cell lines. However, the expression level of miR-96 was not related to the age, gender, clinical stage and histological subtype of the NSCLC patients. GO analysis of 78 predicted target genes of miR-96 showed that 42 of the obtained GO terms are highly associated with specific cellular processes including response to stimulus, signaling pathway, cell division, cell communication, cell migration and calcium signaling. KEGG results indicated that the miR-96 targets are mainly involved in the GnRH signaling pathway, long-term potentiation and insulin signaling pathway. In conclusion, miR-96, functioning as an oncogene, may play an important role in the development and progression of lung cancer. miR-96 may have the potential to serve as a molecular biomarker for the early diagnosis of NSCLC.

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miR-96 functions as a tumor suppressor gene by targeting NUAK1 in pancreatic cancer

Cited by 52 publications

References 30 publications

Retracted: LncRNA UCA1 impacts cell proliferation, invasion, and migration of pancreatic cancer through regulating miR‐96/FOXO3

Retracted: LncRNA UCA1 impacts cell proliferation, invasion, and migration of pancreatic cancer through regulating miR‐96/FOXO3

Nuclear Transport Factor 2 (NTF2) suppresses metastatic melanoma by modifying cell migration, metastasis, and gene expression

Identification and characterization of miR-96, a potential biomarker of NSCLC, through bioinformatic analysis

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