Mirabijalone E: A novel rotenoid from Mirabilis himalaica inhibited A549 cell growth in vitro and in vivo

Linghu, Lang; Fan, Haojun; Hu, Yijie; Zou, Y H; Yang, Panpan; Lan, Xiaozhong; Liao, Zhihua; Chen, Min

doi:10.1016/j.jep.2014.05.034

Cited by 27 publications

(17 citation statements)

References 18 publications

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“…Most of these metabolites, including benzoic acid (Curini et al 2012 ; Jeong et al 2014 ), salicylic acid (Kong et al 2008 ), chlorogenic acid (Jeong et al 2014 ), p-coumaric acid (Lou et al 2012 ), ferulic acid (Zhu et al 2014 ), and naringenin (Rani et al 2016 ) have antibacterial, anti-inflammatory, and anti-oxidative effects. Moreover, p-coumaric acid (Jaganathan et al 2013 ), ferulic acid (Ou and Kwok 2004 ), naringenin (Harmon and Patel 2004 ), apigenin (King et al 2010 ) and rotenoid (Linghu et al 2014 ) exhibit anticancer properties. The important medicinal efficacy of these compounds is related to the potential accumulation of active medicinal compounds in M. himalaica .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, insufficient data on the critical metabolites in M. himalaica has severely hindered the screening of its active medicinal compounds. On the other hand, many studies have shown that rotenoid, which exhibits remarkable anticancer effects, is among the active medicinal compounds in M. himalaica (Fan 2012 ; Linghu et al 2014 ; Lan et al 2016 ). However, the synthesis mechanism of rotenoid and its related metabolites in M. himalaica remains unclear because of a lack of available genetic information, especially at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

“…Rotenoids are isoflavonoids and have important medicinal value, including marked anti-oxidant, antitumour, antibacterial, anti-inflammatory effects. Linghu et al ( 2014 ) reported that rotenoid can block the S phase of the A549 cell cycle, which has important potential for cancer treatment (Linghu et al 2014 ). Using rotenoid extracted from the roots of Boerhavia diffusa , Bairwa et al ( 2013 ) found that rotenoid exhibits clear inhibitory effects on COX-1 and COX-2 activity (Bairwa et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica

Zhang

Hong

et al. 2017

Mol Genet Genomics

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Mirabilis himalaica (Edgew.) Heimerl is among the most important genuine medicinal plants in Tibet. However, the biosynthesis mechanisms of the active compounds in this species are unclear, severely limiting its application. To clarify the molecular biosynthesis mechanism of the key representative active compounds, specifically rotenoid, which is of special medicinal value for M. himalaica, RNA sequencing and TOF-MS technologies were used to construct transcriptomic and metabolomic libraries from the roots, stems, and leaves of M. himalaica plants collected from their natural habitat. As a result, each of the transcriptomic libraries from the different tissues was sequenced, generating more than 10 Gb of clean data ultimately assembled into 147,142 unigenes. In the three tissues, metabolomic analysis identified 522 candidate compounds, of which 170 metabolites involved in 114 metabolic pathways were mapped to the KEGG. Of these genes, 61 encoding enzymes were identified to function at key steps of the pathways related to rotenoid biosynthesis, where 14 intermediate metabolites were also located. An integrated analysis of metabolic and transcriptomic data revealed that most of the intermediate metabolites and enzymes related to rotenoid biosynthesis were synthesized in the roots, stems and leaves of M. himalaica, which suggested that the use of non-medicinal tissues to extract compounds was feasible. In addition, the CHS and CHI genes were found to play important roles in rotenoid biosynthesis, especially, since CHS might be an important rate-limiting enzyme. This study provides a hypothetical basis for the screening of new active metabolites and the metabolic engineering of rotenoid in M. himalaica.Electronic supplementary materialThe online version of this article (10.1007/s00438-017-1409-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica

Zhang

Hong

et al. 2017

Mol Genet Genomics

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“…Mirabilis himalaica is a well‐known herbal plant species that grows on the very high mountains of Tibet Plateau . The taproots of M. himalaica plants have been widely used to treat many diseases, such as nephritic edema, gonorrhea, and stomach disorders, by Tibetan native people for more than 1,000 years .…”

Section: Introductionmentioning

confidence: 99%

“…). The typical bioactive compound of M. himalaica is the rotenoid that is reported to have anti‐tumor activity . Because of slow growth, taproots as only medicinal source and artificial cultivation not available, M. himalaica is endangered.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and transgenic characterization of the genes encoding chalcone synthase and chalcone isomerase from the Tibetan herbal plant Mirabilis himalaica

Lan

Hong

Xia

et al. 2015

Biotech and App Biochem

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Mirabilis himalaica is an endangered medicinal plant species in the Tibetan Plateau. The two genes respectively encoding chalcone synthase (MhCHS) and chalcone isomerase (MhCHI) were isolated and characterized from M. himalaica. The sequence analysis revealed that the two genes were similar with their corresponding homologous genes in other plants. The tissue profiles showed that both MhCHS and MhCHI had higher expression levels in roots than in stems and leaves. Transgenic hairy root cultures respectively with overexpressing MhCHS and MhCHI were established. The genomic PCR detection confirmed the authority of transgenic hairy root lines, in which either MhCHS or MhCHI expression levels were much higher than that in non-transgenic hairy root line. Finally, the HPLC detection results demonstrated that the rotenoid contents in MhCHS/MhCHI-transformed hairy root lines were enhanced. This study provided two candidate genes that could be used to genetic engineering rotenoid biosynthesis in M. himalaica and an alternative method to produce rotenoid using transgenic hairy root cultures.

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Integration of Metabolomics with Network Pharmacology Deciphers the Anti‐Fatigue Activity Mechanisms of the Extract of Mirabilis himalaica Root

Li,

Hao,

Zhou

et al. 2024

Molecular Nutrition Food Res

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Fatigue, a common symptom in both diseased and healthy individuals, is a biological phenomenon characterized by a sense of extreme physical or mental exhaustion. To explore novel drugs and food sources of anti‐fatigue, the hydroalcoholic extract of the root of Mirabilis himalaica (MH extract) is evaluated as anti‐fatigue agents in this work, and clarifies that the mechanism of MH intervention in fatigue symptoms, and distribution of the anti‐fatigue constituents in the plant of Mirabilis himalaica is examined. The results show that the MH extract have a significantly anti‐fatigue effect via the pharmacological experiment and biochemical indicators. The network pharmacology, metabolomics, molecular docking, and pharmacology are integrated to determine that boeravinone A, B, and E are the pharmacoperones of anti‐fatigue. Moreover, the compounds of boeravinone are present only in the root and not in the leaf and stem of the Mirabilis himalaica, which validates that root of Mirabilis himalaica is historically and officially utilized medicinal parts.

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Mirabijalone E: A novel rotenoid from Mirabilis himalaica inhibited A549 cell growth in vitro and in vivo

Cited by 27 publications

References 18 publications

Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica

Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica

Molecular cloning and transgenic characterization of the genes encoding chalcone synthase and chalcone isomerase from the Tibetan herbal plant Mirabilis himalaica

Integration of Metabolomics with Network Pharmacology Deciphers the Anti‐Fatigue Activity Mechanisms of the Extract of Mirabilis himalaica Root

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