Miracle tree (Moringa oleifera) extract prevents rotenone-induced stunting in zebrafish (Danio rerio) through decreasing caspase-3

Khotimah, Husnul; Alfred, Travis; Wangta, Claudio; Chandra, Krisna; Tjan, Benny; Christianto, Albert; Movafeghi, Ali; Kalsum, Umi

doi:10.1063/5.0053014

Cited by 2 publications

(1 citation statement)

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“…Thus, it is possible to observe effects on the size of the larvae, but no behavioral changes. A similar result was observed in the work by Khotimah et al (2021) in which the authors exposed zebra sh embryos at 3 dpf to a concentration of 12.5 µg/L of rotenone and observed a reduction in the body size of zebra sh larvae at 6 dpf. Based on the data obtained in our tests, the morphometric evaluation of the head and body of zebra sh larvae exposed to rotenone as a PD-like model can be a useful tool when performing tests to evaluate drugs that have a protective action against PD-like inducing agents.…”

Section: Toxicological Parameterssupporting

confidence: 87%

Evaluation of toxicity and non-motor symptoms of Parkinson-like induced by rotenone in zebrafish animal model

Andrade

Silva

Soares

et al. 2023

Preprint

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In this study, the effects of exposure to rotenone on development were evaluated, particularly teratogenic and behavioral endpoints in the early life stages of zebrafish. This can serve as a model for Parkinson-like motor and non-motor symptoms, and anxiety-like behavior. The endpoints of percent epiboly, teratogenic effects, mortality, morphometry, thigmotaxis (TH), touch sensitivity (TS), and optomotor response (OMR) were analyzed in zebrafish embryo-larvae stage exposed to rotenone (5 to 20 µg/L). An increase in mortality of zebrafish was observed at 15 and 20 µg/L rotenone concentrations. The rotenone reduced the percent epiboly and increased the presence of teratogenic effects at concentrations of 10, 15, and 20 µg/L. Head and body size reductions were observed at all rotenone concentrations tested. Anxiety-like behaviors were observed with decreased displays of TH behavior in larvae exposed to 15 and 20 µg/L of rotenone. TS was reduced by 20 µg/L rotenone treatment. OMR and the eye diameter of zebrafish were not affected by rotenone exposure. Our results showed that rotenone has the potential to provoke non-motor symptoms, mainly anxiety-like behaviors, in the zebrafish's early life stages, making it a potential model for the study of Parkinson-like disease.

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Section: Toxicological Parameterssupporting

confidence: 87%