MiRNA-27a controls FBW7/hCDC4-dependent cyclin E degradation and cell cycle progression

Identification of Loop Nucleotide Polymorphisms Affecting MicroRNA Processing and Function

“…The SNP attenuated miR27a's inhibition of its target protein, FBW7 (Fig. 4C), a tumor suppressor that functions as a substrate component for a SCF ubiquitin ligase (Lerner et al, 2011). Thus, it is plausible that SNP rs895819 (A → C) leads to upregulation of the FBW7 protein level by reducing miR-27a processing.…”

Low molecular weight cyclin E is associated with p27-resistant, high-grade, high-stage and invasive bladder cancer

“…For these studies, we used the parental bladder cancer in pediatric acute lymphoblastic leukemia (ALL) has been shown to suppress Fbw7 expression, leading to improper cell cycle progression and DNA replication stress, consistent with dysregulation of cyclin E expression. 11 We have found that deregulation of cyclin E can also occur through post-translational processing of the full-length cyclin E by an elastase-like protease to generate low molecular weight (LMW) isoforms. 12,13 Expression of these LMW isoforms in tumor cells leads to increased genomic instability 12 due to premature activation of CDC25C 14 and shortening of the length of mitosis from nuclear envelope breakdown to prometaphase.…”

miR-27a as an Oncogenic microRNA of Hepatitis B Virus-related Hepatocellular Carcinoma

“…Consistently, we here showed that inhition of miR-27a suppressed proliferation, migration and invasion of both HepG2 and Huh7 cell lines, indicating the oncogenic role of miR-27a in HCC cells. Several studies have identified prohibitin (Liu et al, 2009), ZBTB10 (Mertens-Talcott et al, 2007, Myt1 (Mertens-Talcott et al, 2007), FOXO1 (Guttilla et al, 2009), Sprouty2 ( Ma et al, 2010), FBW7 (Lerner et al, 2011) as miR-27a target genes.…”

“…miR-27a has been found to be an oncogene, which is abnormally upregulated in several types of cancers, including gastric cancer (Liu et al, 2009), colon cancer (Chintharlapalli et al, 2009), pancreatic cancer (Szafranska et al, 2007;Ma et al, 2010), breast cancer (Guttilla et al, 2009), ovarian cancer , and kidney cancer (Gottardo et al, 2007), pediatric acute lymphoblastic leukemia (ALL) (Lerner et al, 2011). It has been demonstrated that miR-27a play important roles in mediating cancer cell proliferation, cell cycle, apoptosis, migration and drug resistance (Mertens-Talcott et al, 2007;Liu et al, 2009;Ma et al, 2010;Lerner et al, 2011). Consistently, we here showed that inhition of miR-27a suppressed proliferation, migration and invasion of both HepG2 and Huh7 cell lines, indicating the oncogenic role of miR-27a in HCC cells.…”