miRNA-378a as a key regulator of cardiovascular health following engineered nanomaterial inhalation exposure

Hathaway, Quincy A.; Durr, Andrya J.; Shepherd, Danielle L.; Pinti, Mark V.; Brandebura, Ashley N.; Nichols, C.E.; Kunovac, Amina; Goldsmith, William T.; Friend, Sherri; Abukabda, Alaeddin B.; Fink, Garrett K.; Nurkiewicz, Timothy R.; Hollander, John M.

doi:10.1080/17435390.2019.1570372

Cited by 27 publications

(16 citation statements)

References 80 publications

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“…We, and others, have identi ed that maternal exposures to ENM during gestation can initiate developmental onset of disease within the maturing fetus. In laboratory studies, young and adult offspring have been reported to exhibit coronary dysfunction [40,41,42,43,44,45], vascular perturbations [27,45], reproductive consequences [46,47,48,49,50], and neurodevelopmental delays [51,52] after maternal inhalation of engineered nanomaterials during pregnancy. Therefore, particle deposition, and likely particle accumulation and retention, impact offspring health after birth and into adulthood.…”

Section: Discussionmentioning

confidence: 99%

Nanopolystyrene Translocation and Fetal Deposition After Acute Lung Exposure During Late-Stage Pregnancy

Fournier

D’Errico

Adler

et al. 2020

Preprint

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Abstract Background: Plastic is everywhere. It is used in food packaging, storage containers, electronics, furniture, clothing, and common single-use disposable items. Microplastic and nanoplastic particulates are formed from bulk fragmentation and disintegration of plastic pollution. Plastic particulates have recently been detected in indoor air and remote atmospheric fallout. Due to their small size, microplastic and nanoplastic particulate in the atmosphere can be inhaled and may pose a risk for human health, specifically in susceptible populations. When inhaled, nanosized particles have been shown to translocate across pulmonary cell barriers to secondary organs, including the placenta. However, the potential for maternal-to-fetal translocation of nanosized-plastic particles and the impact of nanoplastic deposition and accumulation on fetal health remain unknown. In this study we investigated whether nanopolystyrene particles can cross the placental barrier and deposit in fetal tissues after maternal pulmonary exposure.Results: Pregnant Sprague Dawley rats were exposed to 20 nm rhodamine-labeled nanopolystyrene beads (2.64 x 1014 particles) via intratracheal instillation on gestational day (GD) 19. Twenty-four hours later on GD 20, maternal and fetal tissues were evaluated using fluorescent optical imaging. Fetal tissues were fixed for particle visualization with hyperspectral microscopy. Using isolated placental perfusion, a known concentration of nanopolystyrene was injected into the uterine artery. Maternal and fetal effluents were collected for 180 minutes and assessed for polystyrene particle concentration. Twenty-four hours after maternal exposure, fetal and placental weights were significantly lower (7% and 8%, respectively) compared with controls. Nanopolystyrene particles were detected in the maternal lung, heart, and spleen. Polystyrene nanoparticles were also observed in the placenta, fetal liver, lungs, heart, kidney, and brain suggesting maternal lung-to-fetal tissue nanoparticle translocation in late stage pregnancy.Conclusion: These studies confirm that maternal pulmonary exposure to nanopolystyrene results in the translocation of plastic particles to placental and fetal tissues and renders the fetoplacental unit vulnerable to adverse effects. These data are vital to the understanding of plastic particulate toxicology and the developmental origins of health and disease.

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Section: Discussionmentioning

confidence: 99%

Nanopolystyrene Translocation and Fetal Deposition After Acute Lung Exposure During Late-Stage Pregnancy

Fournier

D’Errico

Adler

et al. 2020

Preprint

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“…[55] Mitochondrial dysfunction caused by nano-TiO 2 contributes to cardiac dysfunction, partially mediated by overexpression of microRNA-378a. [56] Residing within the first intron of the PGC-1b, [56] microRNA-378a acts as a negative regulator of mitochondrial oxidative metabolism and mitochondrial biogenesis pathways. [57] The expression of MiRNA-378a increased after inhalation of nano-TiO 2 , and the MiRNA-378a knock-out showed a cardioprotective effect.…”

Section: Circulatory and Cardiovascular Systemmentioning

confidence: 99%

“…Thus, fetal mice (prenatally exposed to ≈10 mg m −3 of nano-TiO 2 [54,59] ) showed negligible change in cardiac output. In adult isogenic [59] and heterozygous mice, [56] nano-TiO 2 caused more significant increased cardiac output, whereas in adult wild type and MiRNA-378a knockout mice the cardiac output notably decreased in response to nano-TiO 2 inhalation ( Figure 1).…”

Section: Circulatory and Cardiovascular Systemmentioning

confidence: 99%

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Luo

Xie

et al. 2020

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133

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Titanium dioxide nanoparticles (nano‐TiO2) are widely used in consumer products, raising environmental and health concerns. An overview of the toxic effects of nano‐TiO2 on human and environmental health is provided. A meta‐analysis is conducted to analyze the toxicity of nano‐TiO2 to the liver, circulatory system, and DNA in humans. To assess the environmental impacts of nano‐TiO2, aquatic environments that receive high nano‐TiO2 inputs are focused on, and the toxicity of nano‐TiO2 to aquatic organisms is discussed with regard to the present and predicted environmental concentrations. Genotoxicity, damage to membranes, inflammation and oxidative stress emerge as the main mechanisms of nano‐TiO2 toxicity. Furthermore, nano‐TiO2 can bind with free radicals and signal molecules, and interfere with the biochemical reactions on plasmalemma. At the higher organizational level, nano‐TiO2 toxicity is manifested as the negative effects on fitness‐related organismal traits including feeding, reproduction and immunity in aquatic organisms. Bibliometric analysis reveals two major research hot spots including the molecular mechanisms of toxicity of nano‐TiO2 and the combined effects of nano‐TiO2 and other environmental factors such as light and pH. The possible measures to reduce the harmful effects of nano‐TiO2 on humans and non‐target organisms has emerged as an underexplored topic requiring further investigation.

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“…We, and others, have identi ed that maternal exposures to metallic and carbonaceous ENM during gestation can initiate developmental onset of disease within the maturing fetus. In laboratory studies, young and adult offspring have been reported to exhibit coronary dysfunction [33,34,35,36,37,38], vascular perturbations [38,39], negative reproductive health outcomes [40,41,42], and neurological outcomes [43,44,45] after maternal inhalation of engineered nanomaterials during pregnancy. It is also plausible that these ndings represent a snapshot of time, wherein the particles reach the fetal tissues within 24 hours of exposure but are removed from the fetal circulation prior to birth.…”

Section: Discussionmentioning

confidence: 99%

Nanopolystyrene translocation and fetal deposition after acute lung exposure during late-stage pregnancy

Fournier

D’Errico

Adler

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Plastic is everywhere. It is used in food packaging, storage containers, electronics, furniture, clothing, and common single-use disposable items. Microplastic and nanoplastic particulates are formed from bulk fragmentation and disintegration of plastic pollution. Plastic particulates have recently been detected in indoor air and remote atmospheric fallout. Due to their small size, microplastic and nanoplastic particulate in the atmosphere can be inhaled and may pose a risk for human health, specifically in susceptible populations. When inhaled, nanosized particles have been shown to translocate across pulmonary cell barriers to secondary organs, including the placenta. However, the potential for maternal-to-fetal translocation of nanosized-plastic particles and the impact of nanoplastic deposition or accumulation on fetal health remain unknown. In this study we investigated whether nanopolystyrene particles can cross the placental barrier and deposit in fetal tissues after maternal pulmonary exposure.Results: Pregnant Sprague Dawley rats were exposed to 20 nm rhodamine-labeled nanopolystyrene beads (2.64 x 1014 particles) via intratracheal instillation on gestational day (GD) 19. Twenty-four hours later on GD 20, maternal and fetal tissues were evaluated using fluorescent optical imaging. Fetal tissues were fixed for particle visualization with hyperspectral microscopy. Using isolated placental perfusion, a known concentration of nanopolystyrene was injected into the uterine artery. Maternal and fetal effluents were collected for 180 minutes and assessed for polystyrene particle concentration. Twenty-four hours after maternal exposure, fetal and placental weights were significantly lower (7% and 8%, respectively) compared with controls. Nanopolystyrene particles were detected in the maternal lung, heart, and spleen. Polystyrene nanoparticles were also observed in the placenta, fetal liver, lungs, heart, kidney, and brain suggesting maternal lung-to-fetal tissue nanoparticle translocation in late stage pregnancy.Conclusion: These studies confirm that maternal pulmonary exposure to nanopolystyrene results in the translocation of plastic particles to placental and fetal tissues and renders the fetoplacental unit vulnerable to adverse effects. These data are vital to the understanding of plastic particulate toxicology and the developmental origins of health and disease.

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miRNA-378a as a key regulator of cardiovascular health following engineered nanomaterial inhalation exposure

Cited by 27 publications

References 80 publications

Nanopolystyrene Translocation and Fetal Deposition After Acute Lung Exposure During Late-Stage Pregnancy

Nanopolystyrene Translocation and Fetal Deposition After Acute Lung Exposure During Late-Stage Pregnancy

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Nanopolystyrene translocation and fetal deposition after acute lung exposure during late-stage pregnancy

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miRNA-378a as a key regulator of cardiovascular health following engineered nanomaterial inhalation exposure

Cited by 27 publications

References 80 publications

Nanopolystyrene Translocation and Fetal Deposition After Acute Lung Exposure During Late-Stage Pregnancy

Nanopolystyrene Translocation and Fetal Deposition After Acute Lung Exposure During Late-Stage Pregnancy

Rethinking Nano‐TiO2 Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Nanopolystyrene translocation and fetal deposition after acute lung exposure during late-stage pregnancy

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Product

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Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals