miRNA and mRNA expression profiling reveals potential biomarkers for metastatic cutaneous melanoma

Wang, Jun; Tao, Yiye; Bian, Queqiao

doi:10.1080/14737140.2021.1882860

Cited by 8 publications

(10 citation statements)

References 46 publications

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“…Predicted target genes of the most strongly upregulated miRNA, miR-4426, which is so far unknown in cancer (Figure 7A), are implicated in the canonical Wnt signaling pathway, positive regulation of locomotion, positive regulation of cell migration, and positive regulation of cell motility (Supplementary Table S1). miR-4426 shares 417 targets with miR-203a-3p (Figure 7B), a miRNA known in melanoma as a biomarker for metastatic melanoma [61], but the functional role of this miRNA is unknown so far. These target genes are involved in the regulation of transmembrane receptor protein serine/threonine kinase signaling pathway, cell population proliferation, and positive regulation of cell differentiation (Supplementary Table S1).…”

Section: Mirnas Differentially Expressed In Only Metastasis-derived Melanoma Cell Lines Compared To Melanoblasts Provide Information Aboumentioning

confidence: 99%

Learning from Embryogenesis—A Comparative Expression Analysis in Melanoblast Differentiation and Tumorigenesis Reveals miRNAs Driving Melanoma Development

Linck-Paulus

Lämmerhirt

Völler

et al. 2021

JCM

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Malignant melanoma is one of the most dangerous tumor types due to its high metastasis rates and a steadily increasing incidence. During tumorigenesis, the molecular processes of embryonic development, exemplified by epithelial–mesenchymal transition (EMT), are often reactivated. For melanoma development, the exact molecular differences between melanoblasts, melanocytes, and melanoma cells are not completely understood. In this study, we aimed to identify microRNAs (miRNAs) that promote melanoma tumorigenesis and progression, based on an in vitro model of normal human epidermal melanocyte (NHEM) de-differentiation into melanoblast-like cells (MBrCs). Using miRNA-sequencing and differential expression analysis, we demonstrated in this study that a majority of miRNAs have an almost equal expression level in NHEMs and MBrCs but are significantly differentially regulated in primary tumor- and metastasis-derived melanoma cell lines. Further, a target gene analysis of strongly regulated but functionally unknown miRNAs yielded the implication of those miRNAs in many important cellular pathways driving malignancy. We hypothesize that many of the miRNAs discovered in our study are key drivers of melanoma development as they account for the tumorigenic potential that differentiates melanoma cells from proliferating or migrating embryonic cells.

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Section: Mirnas Differentially Expressed In Only Metastasis-derived Melanoma Cell Lines Compared To Melanoblasts Provide Information Aboumentioning

confidence: 99%

Learning from Embryogenesis—A Comparative Expression Analysis in Melanoblast Differentiation and Tumorigenesis Reveals miRNAs Driving Melanoma Development

Linck-Paulus

Lämmerhirt

Völler

et al. 2021

JCM

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“…Thus, miR-129-2-3p and let-7e-5p were overexpressed in MNT-1 but down-regulated in VMM1, while miR-100-5p, miR-125b-5p, let-7a-5p, let-7d-5p, let-7f-5p and let-7g-5p registered a much higher expression level in VMM1, but decreased significantly in MNT-1 compared to MelJuSo cells. Data currently available reveal that miR-100-5p and miR-125b-5p can promote tumor cell proliferation and invasion, at the same time being related to survival time as they can negatively affect patient response to treatment with BRAF inhibitors such as vemurafenib [ 6 , 9 , 30 ]. Additionally, together with let-7e-5p, they can influence the differentiation and polarization of monocytes towards the immunosuppressive phenotype (MDSCs), indicating a potential negative effect on immunotherapy [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…Despite this significant accumulation of knowledge, it has yet to yield considerable clinical benefits in terms of improved treatment options or overall patient survival [ 8 , 9 ]. As such, it remains an active field of research for scientists in pursuit of a complete overview of melanoma metastasis.…”

Section: Introductionmentioning

confidence: 99%

Comparative Expression Profiling Reveals Molecular Markers Involved in the Progression of Cutaneous Melanoma towards Metastasis

Lazăr

Dinescu

Sleiman

et al. 2023

IJMS

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Cutaneous melanoma is one of the most aggressive types of cancer and often proves fatal in metastatic stages. Few treatment options are available, and its global incidence is quickly increasing. In order to gain an improved understanding of the molecular features regarding melanoma progression, we have compared gene and small non-coding RNA expression profiles from cell lines derived from primary melanoma (MelJuSo), lymph node metastasis (MNT-1) and brain metastasis (VMM1), representing distinct stages of malignant progression. Our preliminary results highlighted the aberrant regulation of molecular markers involved in several processes that aid melanoma progression and metastasis development, including extracellular matrix remodeling, migratory potential and angiogenesis. Moreover, bioinformatic analysis revealed potential targets of the microRNAs of interest. Confocal microscopy and immunohistochemistry analysis were used for validation at the protein level. Exploring the molecular landscape of melanoma may contribute to the achievement of future efficient targeted therapy, as well as better prevention, diagnosis and clinical management.

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“…Previous studies have shown that cytokinecytokine receptor interaction is significantly associated with various CM processes, such as exercise therapy and CM metastasis-related microRNA and mRNA expression. This interaction may play a fundamental role in modulating tumor metastasis, which is a critical factor affecting the OS of patients with CM [32][33][34]. Further, the chemokine [35] and B cell receptor signaling pathways [36,37] are also important factors that affect the prognosis of patients with CM.…”

Section: Discussionmentioning

confidence: 99%

Prediction of clinical prognosis in cutaneous melanoma using an immune-related gene pair signature

Long

et al. 2021

Bioengineered

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Cutaneous melanoma (CM) is a malignant and aggressive skin cancer that is the leading cause of skin cancer-related deaths. Increasing evidence shows that immunity plays a vital role in the prognosis of CM. In this study, we developed an immune-related gene pair (IRGP) signature to predict the clinical prognosis of patients with CM. Immune-related genes from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases were selected to construct the IRGPs, and patients with CM in these two cohorts were assigned to low-and highrisk subgroups. Moreover, we investigated the IRGPs and their individualized prognostic signatures using Kaplan-Meier survival analysis, univariate and multivariate Cox analyses, and analysis of immune cell infiltration in CM. A 41-IRGP signature was constructed from 2498 immune genes that could significantly predict the overall survival of patients with CM in both the TCGA and GEO cohorts. Immune infiltration analysis indicated that several immune cells, especially M1 macrophages and activated CD4 T cells, were significantly associated with the prognostic effect of the IRGP signature in patients with CM. Overall, the IRGP signature constructed in this study was useful for determining the prognosis of patients with CM and for providing further understanding of CM immunotherapy.

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miRNA and mRNA expression profiling reveals potential biomarkers for metastatic cutaneous melanoma

Cited by 8 publications

References 46 publications

Learning from Embryogenesis—A Comparative Expression Analysis in Melanoblast Differentiation and Tumorigenesis Reveals miRNAs Driving Melanoma Development

Learning from Embryogenesis—A Comparative Expression Analysis in Melanoblast Differentiation and Tumorigenesis Reveals miRNAs Driving Melanoma Development

Comparative Expression Profiling Reveals Molecular Markers Involved in the Progression of Cutaneous Melanoma towards Metastasis

Prediction of clinical prognosis in cutaneous melanoma using an immune-related gene pair signature

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