miRNA Expression Profile and Involvement of Let-7d-APP in Aged Rats with Isoflurane-Induced Learning and Memory Impairment

Luo, Ting; Yin, Shuzhou; Shi, Rong; Xu, Congcong; Wang, Yun; Cai, Jun; Yue, Yun; Wu, Anshi

doi:10.1371/journal.pone.0119336

Cited by 20 publications

(16 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Let-7d is abundant in the brain. In fact, using a microarray screening, Shu and colleagues found that let-7d was up-regulated in the hippocampus and the Marginal division (MrD) of the neostriatum ( Shu et al, 2013 ), suggesting that it may play an important role in modulating learning and memory ( Luo et al, 2015 ). Also, let-7d, which is increased in the prefrontal cortex of spontaneously hypertensive rats' brain, negatively regulated galectin-3 which was associated with attention deficit hyperactivity disorder development ( Wu et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Lentiviral-mediated let-7d microRNA overexpression induced anxiolytic- and anti-depressant-like behaviors and impaired dopamine D3 receptor expression

Bahí

Dreyer

2018

European Neuropsychopharmacology

View full text Add to dashboard Cite

Generalized anxiety and major depression disorders (MDD) are severe debilitating mood disorders whose etiology are not fully understood, but growing evidence indicates that microRNAs (miRNAs) might play a key role in their neuropathophysiological mechanisms. In the current study, we investigate the role of Lethal-7 (let-7d) miRNA, and its direct target dopamine D3 receptor (D3R) gain-of-function, in the hippocampus, in preclinical models of anxiety and depression in mice. For this purpose, we have constructed a lentiviral vector carrying let-7d miRNA and its anxiolytic effect was investigated by employing the open-field (OF) and the elevated plus maze (EPM) tests. The anti-depressant activity was evaluated using the tail suspension and the forced-swim tests (TST & FST). Our results show that let-7d overexpression significantly improved the measures of anxiety in the OF and EPM tests. In addition, let-7d increased the mobility time in the TST and FST. Interestingly, gene expression interaction analysis shows that the D3R mRNA negatively correlates with let-7d expression. In a different set of experiments, we used a tetracycline-inducible (tet-off) lentiviral vector to overexpress D3R to assess its gain-of-function in the hippocampus on anxiety-and depression-like behaviors. In line, we found that in the absence of doxycycline, D3R produced a significant anxiogenic and depressant-like response. Most importantly, these effects were abrogated when mice were fed doxycycline in drinking water. Our results provide the first evidence for an anxiolytic and

show abstract

Section: Introductionmentioning

confidence: 99%

Lentiviral-mediated let-7d microRNA overexpression induced anxiolytic- and anti-depressant-like behaviors and impaired dopamine D3 receptor expression

Bahí

Dreyer

2018

European Neuropsychopharmacology

View full text Add to dashboard Cite

show abstract

“…Anesthesia-induced cognitive impairment is a recognized clinical phenomenon and possibly the most frequent type of postoperative cognitive impairment ( 3 , 4 ). miRNAs had been reported to be associated with cognitive ability ( 34 , 35 ). The present study aimed to investigate the effect of miR-383 expression on propofol-induced learning and memory impairment.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment

et al. 2018

View full text Add to dashboard Cite

Anesthesia-induced cognitive impairment is a recognized clinical phenomenon. The present study aimed to investigate the effect of microRNA-383 (miR-383) expression on propofol-induced learning and memory impairment. In total, 48 male Sprague-Dawley rats (weight, 250±10 g) were randomly divided into four groups (n=12 each): Control group, and three groups of rats that were anesthetized with propofol for 6 h and untreated (propofol model group), treated with a constructed lentivirus vector expressing miR-383 mimics (mimic + propofol group), or treated with miR-383 scramble (scramble + propofol group). The learning memory ability, hippocampal neuron apoptosis and expression of apoptosis-associated factors were detected using reverse transcription-quantitiative polymerase chain reaction and western blot analysis. Propofol treatment significantly reduced the relative mRNA and protein expression of miR-383, induced neuron apoptosis, upregulated the Bax/Bcl-2 ratio, downregulated the relative mRNA and protein expression levels of postsynaptic density protein 95 and cAMP-response element binding protein, and inactivated the phosphoinositide 3-kinase/protein kinase B signaling pathway. By contrast, miR-383 mimics significantly altered the propofol-induced dysregulation of the aforementioned factors. In conclusion, miR-383 mimic was able to repair propofol-induced cognitive impairment via protecting against hippocampal neuron apoptosis and dysregulation of related factors. The present study suggested that miR-383 may be used as a potential therapeutic target for the clinical treatment of cognitive impairment induced by propofol anesthesia.

show abstract

“…Finally, AGO2 immunoprecipitation (AGO2-IP) assays were used to demonstrate that the sponges were enriched in the AGO2-IP fraction [39]. Given that miRNAs have been implicated not only in cancer but in numerous neurological [42]- [44], cardiovascular [45]- [47] and autoimmune diseases [48]- [50], the miRNA sponges could be another useful tool in the arsenal for combatting disease.…”

Section: Health Relevance and Potential Uses In Biomedicinementioning

confidence: 99%

Circular RNAs: New Players in Gene Regulation

Andreeva¹,

Cooper²

2015

ABB

View full text Add to dashboard Cite

The existence of circular RNAs (circRNAs) was demonstrated over 30 years ago. They did not gain much interest at the time because they appeared to be relatively rare when compared to the abundance of the canonical linear RNAs. However, more recent evidence suggests that circRNAs are abundant in cells and tissues and possess intriguing biological properties. These recent developments have renewed our interest in this novel class of molecules. This report will provide an overview of circRNAs, discuss how they may modify our understanding of gene regulation and indicate their most likely relevance to health. The circRNAs from viruses, bacteria and archaea are not in the scope of this report, and we focused this review on circRNAs in eukaryotes.

show abstract

miRNA Expression Profile and Involvement of Let-7d-APP in Aged Rats with Isoflurane-Induced Learning and Memory Impairment

Cited by 20 publications

References 30 publications

Lentiviral-mediated let-7d microRNA overexpression induced anxiolytic- and anti-depressant-like behaviors and impaired dopamine D3 receptor expression

Lentiviral-mediated let-7d microRNA overexpression induced anxiolytic- and anti-depressant-like behaviors and impaired dopamine D3 receptor expression

MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment

Circular RNAs: New Players in Gene Regulation

Contact Info

Product

Resources

About