miRNA signatures can predict acute liver failure in hepatitis E infected pregnant females

Trehanpati, Nirupma; Sehgal, Rashi; Patra, Soumya; Vyas, Ashish Kumar; Vasudevan, Madavan; Khosla, Ritu; Khanam, Arshi; Kumar, Guresh; Maiwall, Rakhi; Ramakrishna, Gayatri; Kottilil, Shyam; Sarin, Shiv Kumar

doi:10.1016/j.heliyon.2017.e00287

Cited by 19 publications

(11 citation statements)

References 40 publications

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“…Yamaguchi et al 13 found that miR-431 could contribute to chemotherapy resistance in human cancer. In addition, Trehanpati et al 15 found that miR-431 was significantly expressed in hepatitis E viral (HEV) infection in liver. Other studies have also explored the relationship between miR-431 and liver disease such as HCC, but whether miR-431-5p can play a part in the invasion of HCC by means of the EMT and the mechanism of how miR-431-5p reduces the EMT in HCC have not been fully explored up to now in vitro or in vivo.…”

Section: Introductionmentioning

confidence: 99%

miR-431-5p alters the epithelial-to-mesenchymal transition markers by targeting <em>UROC28</em> in hepatoma cells

Kong

Han

Deng

et al. 2018

OTT

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ObjectiveMicroRNA (miR)-431 plays an essential role in various human cancer types, particularly in the process of invasion. However, the function and mechanism of miR-431-5p in the invasion of hepatocellular carcinoma (HCC) remain undefined.MethodsThe expression levels of miR-431-5p and its potential target protein UROC28 in hepatocellular carcinoma cells and tissues were detected, and the levels of EMT markers in vivo and in vitro were also detected.ResultsMiR-431-5p was downregulated in HCC cell lines and tissues and associated with vascular invasion and tumor encapsulation. Furthermore, miR-431-5p was able to influence the epithelialto-mesenchymal transition (EMT) process in HCCLM3 and HUH7 cells. Mechanistically, it was discovered that miR-431-5p repressed invasion by targeting UROC28. Furthermore, miR-431-5p influenced the EMT markers in HCCLM3 and HUH7 cells by downregulating UROC28 expression. Similarly, in vivo assays confirmed that miR-431-5p upregulation in HCC cells remarkably inhibited tumor proliferation and influenced the EMT markers.ConclusionThe current study has demonstrated that the miR-431-5p/UROC28 axis acts possible influence on the EMT in HCC. Upregulation of miR-431-5p could be an original approach for inhibiting tumor invasion.

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Section: Introductionmentioning

confidence: 99%

miR-431-5p alters the epithelial-to-mesenchymal transition markers by targeting <em>UROC28</em> in hepatoma cells

Kong

Han

Deng

et al. 2018

OTT

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“…Healthy pregnancy was found to be associated with lower miR-122 levels. Notably, pregnancy was shown to significantly reduce more than 50% of PBMC-miRNAs compared to NPR controls [35]. When healthy pregnant women were taken as controls, a different pattern emerged; serum miR-122 levels varied according to the clinical presentation.…”

Section: Discussionmentioning

confidence: 99%

Circulating miR-122 levels in self-recovering hepatitis E patients

Haldipur

Arankalle

2019

ExRNA

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Background: Hepatitis E (HE) is prevalent in developing countries in both epidemic and sporadic forms and is characterized by high mortality during pregnancy. miR-122, the major hepatic microRNA has been shown to be modulated during liver diseases. Lack of data in HE led to investigations in non-pregnant (NPR) and pregnant (PR) patients. Results: Self-recovering NPR patients and pregnant women presenting with clinical (PR-acute) or subclinical (PR-SC) HE and respective healthy controls were studied. Serum samples were tested for miR-122 levels using qRT-PCR. Acute-phase, NPR patients circulated lower miR-122 levels, reducing further during convalescence. In contrast to previous reports, circulating miR-122 levels did not correlate with serum aminotransferase (ALT). In PR-acute patients, miR-122 levels were significantly lower that reflected pregnancy status and not HEV effect. In PR-SC patients, miR-122 levels were lower than the pregnant controls and reduced further when examined one month apart. A pregnant, fulminant HE patient circulated very high miR-122 levels that increased further during convalescence. Correlation analysis of miR-122 and circulating cytokines showed moderate correlation with CCL2 (subclinical, pregnant) and IL-6 (NPR). This first report revealed downregulation of circulating miR-122 levels in self-recovering NPR-acute patients despite liver damage (raised serum ALT) suggestive of alternate mechanism of secretion in blood. Conclusion: HEV infection during pregnancy led to differential modulation of serum miR-122 levels that correlated with clinical presentation. Utility of miR-122 as a prognostic marker for severe disease during pregnancy needs to be evaluated.

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“…8 Its premature form miR-450b is predominantly upregulated in liver tissue with acute hepatic failure caused by viral hepatitis E, and is associated with poor prognosis in pregnant women. 9 These studies suggest that miR-450b and miR-450b-3p may also play important roles in HCC. Previous research showed that miR-450b-3p could inhibit breast cancer proliferation by targeting the 3′UTR of human epidermal growth factor receptor 3 (HER3) mRNA.…”

Section: Introductionmentioning

confidence: 94%

“…Among numerous tumor‐associated miRNAs, miR‐450b‐3p, whose precursor (pre‐miRNA) is located on chromosome Xq26.3, has been shown to be downregulated in the liver of the Excision Repair Cross‐Complementation Group 1 ( ERCC1 )‐deficient progeroid mouse model and the old wild‐type mice, which suggests the level of miR‐450b‐3p in the liver is related to aging . Its premature form miR‐450b is predominantly upregulated in liver tissue with acute hepatic failure caused by viral hepatitis E, and is associated with poor prognosis in pregnant women . These studies suggest that miR‐450b and miR‐450b‐3p may also play important roles in HCC.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‐450b‐3p inhibits cell growth by targeting phosphoglycerate kinase 1 in hepatocellular carcinoma

Chen

Zhuang

Wang

et al. 2019

J of Cellular Biochemistry

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Dysregulation of microRNAs frequently contributes to the occurrence and progression of human diseases, including hepatocellular carcinoma (HCC). In this study, the role of miR‐450b‐3p in HCC was investigated. Gene Expression Omnibus database and HCC specimens were used to evaluate the expression level of miR‐450b‐3p and the patient's prognosis. Cell functional analyses and tumor xenograft model were used to assess the role of miR‐450b‐3p in HCC. Bioinformatics was used to predict the downstream target gene of miR‐450b‐3p, which was verified by dual‐luciferase reporter assay. MiR‐450b‐3p was found to be downregulated in HCC cell lines and tissues, compared with nontransformed immortal hepatic cells and adjacent normal liver tissues, respectively. Lower expression of miR‐450b‐3p was associated with poor overall survival and disease‐free survival in patients with HCC. Ectopic expression of miR‐450b‐3p inhibited HCC cell viability, colony formation, and cell‐cycle progression in vitro, and suppressed the growth of HCC xenograft tumors in vivo. Interestingly, a negative correlation between miR‐450b‐3p and phosphoglycerate kinase 1 (PGK1) protein was observed among HCC specimens. Additionally, miR‐450b‐3p inhibited PGK1 expression and phosphorylation of protein kinase B in HCC cell lines. Further experiments confirmed that PGK1 was a direct target of miR‐450b‐3p. Moreover, restoration of PGK1 abrogated the inhibitory effect of miR‐450b‐3p on HCC proliferation and cell division. In conclusion, miR‐450b‐3p is downregulated in human HCC and exerts tumor suppressive effects at least in part by inhibiting PGK1.

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miRNA signatures can predict acute liver failure in hepatitis E infected pregnant females

Cited by 19 publications

References 40 publications

miR-431-5p alters the epithelial-to-mesenchymal transition markers by targeting <em>UROC28</em> in hepatoma cells

miR-431-5p alters the epithelial-to-mesenchymal transition markers by targeting <em>UROC28</em> in hepatoma cells

Circulating miR-122 levels in self-recovering hepatitis E patients

MicroRNA‐450b‐3p inhibits cell growth by targeting phosphoglycerate kinase 1 in hepatocellular carcinoma

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