Miroestrol, a phytoestrogen from Pueraria mirifica, improves the antioxidation state in the livers and uteri of β-naphthoflavone-treated mice

Abstract

Oxidative stress is involved in the progression of several diseases such as diabetes, hypertension, and age-related diseases. Miroestrol (MR) is a potent phytoestrogen from the tuberous root of Pueraria mirifica, a plant used in traditional Thai medicine that is claimed to have rejuvenating effects. In this study, the effects of MR on the antioxidation system, including anti-lipid peroxidation; on the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase; and on glutathione content in the livers and uteri of β-naphthoflavone (BNF)-treated mice were determined. BNF-treated mice are a model of procarcinogen-exposed mice. The results showed that MR improved the antioxidant activities of SOD and CAT in the livers and uteri of both normal and BNF-treated mice, while estradiol (E2) increased SOD activity in the uteri of normal mice and CAT activity in the livers of both normal and BNF-treated mice. In the liver, MR increased the levels of several forms of glutathione, whereas in the uteri E2 and MR reduced the level of lipid peroxidation by decreasing the level of malondialdehyde. Therefore, the use of MR as an alternative hormone replacement therapy might be beneficial due to its ability to improve antioxidation systems.

Imbalance of the antioxidative system by plumbagin and Plumbago indica L. extract induces hepatotoxicity in mice

“…SOD activity was assessed as previously described [ 34 ]. Briefly, a mixture containing liver homogenate, chloroform, and ethanol was centrifuged at 13,000 ×g , 4°C for 30 min.…”

“…CAT activity was assessed by the colorimetric method of Goth (1991) with some modifications [ 34 ]. The liver homogenate was incubated with H 2 O 2 at 37°C for 1 min before adding ammonium molybdate to stop the reaction.…”

Antihypertensive Effects of Apple Peel Extract on Spontaneously Hypertensive Rats

“…However, the enzymes associated with the GPX system i.e., GPX and GR activity did not show any significant difference when compared to the control group. The SOD catalyzes the transformation of ROS to hydrogen peroxide (H 2 O 2 ) which is the initial step of deactivation of ROS 26 . In APE treated mice liver, SOD activity had almost doubled in amount when compared with the control group.…”

“…In APE treated mice liver, SOD activity had almost doubled in amount when compared with the control group. An increased concentration of SOD indicates a higher capacity to scavenge free radicals 26 . Captopril had not shown any significant difference on these parameters when compared with the control.…”

A pilot pharmacokinetic study of miroestrol and deoxymiroestrol on rabbit sera using polyclonal antibody‐based icELISA analysis

“…According to the hypothesis, reaction of specific estrogen metabolites can generate critical DNA mutations which initiate breast, prostate, and other cancers (Russo & Russo, 2006). The effects of ME and DME on estrogenic activity and metabolism have been reported to explore their safety data (Jearapong, Chatuphonprasert, & Jarukamjorn, 2014;Monthakantirat et al, 2014;Udomsuk, Juengwatanatrakul, Putalun, & Jarukamjorn, 2011a, 2011b. It seems that their metabolites might not be the risk factors of hormone dependent cancer-like estrogen.…”