Mise en évidence d’une variation intra spécifique chez <i>Botrytis cinerea</i> et lutte biologique <i>in vitro</i> par l’extrait de compost

Abstract

Detection of Intraspecific variation in Botrytis cinerea and in vitro biological control by compost extract.Objectives: The present study was on intraspecific variation detection in B. cinerea and the effect of a municipal solid waste compost extract on two stages of its life cycle. Methodology and results: tomato leaves were inoculated with 22 strains of B. cinerea to select the most virulent. They were transplanted into culture medium based on compost extract to assess the growth and sporulation. B. cinerea strains isolated from tomato plants proved to be more virulent. Growth and sporulation of five strains selected were inhibited by the extracts in the media by 95.24% and 100% respectively. Conclusion and application of research: The study of pathogenicity of B. cinerea showed the existence of intraspecific variation. The sterilized compost extracts efficiency on B. cinerea in vitro proves that chemical compounds are involved and suggests the possibility of using these extracts and compost in vivo against tomato gray mold. Mouria et al.… J. Appl. Biosci. 2013. Mise en évidence d'une variation intraspecific chez B. cinerea et ce lutte biologique in vitro.

RETRACTED: The efficacy of composts as bio-control agents against damping-off disease incidence caused by Fusarium acuminatum and Fusarium solani

“…The pH measurement, the salinity, and the dry matter content of the composts were assessed by using the standard protocol of Mouria et al, (2014).…”

“…Subsequently, the Petri dishes were incubated in a controlled environment at a consistent temperature of 25°C for 7 days. After this incubation period, the degree of inhibition in mycelia growth was quanti ed using the methodology described in a previous study authored by Mouria et al (2013).…”

“…After 10 days of incubation, the sporulation assay was performed as described by Amadioha (2003) and Mouria et al (2014). Spores germination assay was performed by using micro-culture on coverslip by using the protocol of Rapilly (1968).…”

Effet du compost et de <i>Trichoderma harzianum</i> sur la suppression de la verticilliose de la tomate

“…La présence de plusieurs antagonistes ayant différents modes d'action dans ce compost (Mouria et al, 2013a) justifie son aptitude suppressive élevée qui est liée à sa microflore totale, suppression dite " générale" et à T. harzianum en particulier dite suppression "spécifique" Veeken et al, 2005). Cependant, des facteurs chimiques non identifiés du compost sont aussi responsables de cette activité suppressive (Mouria et al, 2013b). On peut donc conclure que les effets de ce compost sur la verticilliose de la tomate incluent des facteurs biologiques et chimiques.…”

Potentials of compost, compost tea and aqueous neem leaves extract in reducing some diseased plants and boosting onion (Allium cepa L.) production in the field at Gapring-Lara in t he Department of Mayo Kani (Cameroon)

“…Organic fertilizers such as compost and its extracts, or even plant extracts as biopesticide could be used as alternative, because of advantages they have of being biodegradable, non-persistent in the nature, inexpensive, and more accessible to small producers (Mahmoud et al, 2015). The current trend is the formulation of natural substances such as biopesticides and biofertilizers (Sagoua, 2009;Ekra, 2010;Mouria et al, 2013;Traore et al, 2015;Pale et al, 2021). Previous researches carried out in northern Cameroon have shown the effecacy of compost and their extracts in improving growth and yield as well as reducing the incidence of plant diseases (Ngakou et al, 2008;Anguessin et al, 2021).…”