MIST1, an Inductive Signal for Salivary Amylase in Mesenchymal Stem Cells

α-Amylase is abundant in plants and animals. α-Amylase inhibitors can reduce endogenous α-amylase activity, playing an essential role in agricultural pest control, and preventing and treating human disease. In the agricultural field, α-Amylase inhibitors can restrict pest that relies on the starch of crops. Acarbose is an α-amylase inhibitor used to treat diabetes. Some αamylase inhibitors are represented by antinutritional factors, while others are proteinaceous. Depending on their structures and sources, researchers have divided them into seven types: The knottin-like type, the γ-thionin-like type, the cereal type, the Kunitz type, the thaumatin-like type, and the lectin-like type. This paper introduces the methods for separating, purifying, and detecting proteinaceous α-amylase inhibitors while examining the structure and inhibition mechanism of several proteinaceous α-amylase inhibitors. Finally, it explores the potential applications of α-amylase inhibitors.

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“…[41] Furthermore, the MIST-1 signal has been reported to enhance the expression of salivary amylase 1 in vitro . [42]…”

Section: Kindsmentioning

confidence: 99%

Proteinaceous α-amylase inhibitors: purification, detection methods, types and mechanisms

Zhou

Zhang

et al. 2021

International Journal of Food Properties

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“…FN1, in addition to collagen I and II, upregulated in pancreatic exocrine acinar cells corresponding to the overexpression of a protein named muscle, intestine and stomach expression 1 (MIST-1) [42]. It is important to note that our previous study revealed that MIST-1, a basic helix-loop-helix transcription factor for AMY1 expression, was upregulated in co-cultured MSCs [25,26,43]. Additionally, MIST1 was found to be an upstream key regulator of FN1 [44] and other transcription factors like TCF3 [45] and PTF1A [46].…”

Section: Discussionmentioning

confidence: 96%

Secretome Analysis of Inductive Signals for BM-MSC Transdifferentiation into Salivary Gland Progenitors

Mona

Kobeissy

Park

et al. 2020

IJMS

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Severe dry mouth in patients with Sjögren’s Syndrome, or radiation therapy for patients with head and neck cancer, significantly compromises their oral health and quality of life. The current clinical management of xerostomia is limited to palliative care as there are no clinically-proven treatments available. Previously, our studies demonstrated that mouse bone marrow-derived mesenchymal stem cells (mMSCs) can differentiate into salivary progenitors when co-cultured with primary salivary epithelial cells. Transcription factors that were upregulated in co-cultured mMSCs were identified concomitantly with morphological changes and the expression of acinar cell markers, such as α-amylase (AMY1), muscarinic-type-3-receptor(M3R), aquaporin-5(AQP5), and a ductal cell marker known as cytokeratin 19(CK19). In the present study, we further explored inductive molecules in the conditioned media that led to mMSC reprogramming by high-throughput liquid chromatography with tandem mass spectrometry and systems biology. Our approach identified ten differentially expressed proteins based on their putative roles in salivary gland embryogenesis and development. Additionally, systems biology analysis revealed six candidate proteins, namely insulin-like growth factor binding protein-7 (IGFBP7), cysteine-rich, angiogenetic inducer, 61(CYR61), agrin(AGRN), laminin, beta 2 (LAMB2), follistatin-like 1(FSTL1), and fibronectin 1(FN1), for their potential contribution to mMSC transdifferentiation during co-culture. To our knowledge, our study is the first in the field to identify soluble inductive molecules that drive mMSC into salivary progenitors, which crosses lineage boundaries.

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“…Expression of the M3 receptor was detected at both the mRNA and protein level in mouse BM-MSCs [58]. The M3 receptor was localised primarily to the endoplasmic reticulum in the investigated BM-MSCs and as such were not competent to signal.…”

Section: Expression Of Of Machrs In Mscsmentioning

confidence: 90%

Acetylcholine Receptors in Mesenchymal Stem Cells

Alqahtani

Butcher

Ramage

et al. 2023

Stem Cells and Development

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Mesenchymal stem cells (MSCs) are well known for their regenerative potential. Despite the fact that the ability of MSCs to proliferate and differentiate has been studied extensively, there still remains much to learn about the signalling mechanisms and pathways which control proliferation and influence differentiation phenotype. In recent years, there has been growing evidence for the utility of non-neuronal cholinergic signalling systems and that acetylcholine (ACh) plays an important, ubiquitous, role in cell-to-cell communication. Indeed, cholinergic signalling is hypothesised to occur in stem cells and ACh synthesis, as well as ACh receptor (AChR) expression, has been identified in several stem cell populations; including MSCs. Furthermore, AChRs have been found to influence MSC regenerative potential. In humans, there are two major classes of AChRs, muscarinic AChRs and nicotinic AChRs, with each class possessing several subtypes or subunits. In this review, the expression and function of AChRs in different types of MSC will be summarised with the aim of highlighting how AChRs play a pivotal role in regulating MSC regenerative function.

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MIST1, an Inductive Signal for Salivary Amylase in Mesenchymal Stem Cells

Cited by 7 publications

References 55 publications

Proteinaceous α-amylase inhibitors: purification, detection methods, types and mechanisms

Proteinaceous α-amylase inhibitors: purification, detection methods, types and mechanisms

Secretome Analysis of Inductive Signals for BM-MSC Transdifferentiation into Salivary Gland Progenitors

Acetylcholine Receptors in Mesenchymal Stem Cells

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