Mistranslation in IGF-1 during over-expression of the protein in Escherichia coli using a synthetic gene containing low frequency codons

Seetharam, Ramnath; Heeren, Robert A.; Wong, Edith Y.; Braford, Sarah R.; Klein, Barbara K.; Aykent, Serdar; Kotts, Claire E.; Mathis, Karl J.; Bishop, Bruce F.; Jennings, Michael J.; Smith, Christine E.; Siegel, Ned R.

doi:10.1016/s0006-291x(88)81117-3

Cited by 68 publications

(25 citation statements)

References 10 publications

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“…This mistranslation of Arg for Lys has been extensively documented in other proteins [22], [23], [24], [25]. Therefore, it would seem that there are two positions for rare codons that may be critical for the expression of TIM, those at the beginning and the adjacent codons at positions 99 and 100.…”

Section: Discussionmentioning

confidence: 81%

The importance of arginine codons AGA and AGG for the expression in E. coli of triosephosphate isomerase from seven different species

Aguirre-López

Cabrera

Gómez‐Puyou

et al. 2017

Biotechnology Reports

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Section: Discussionmentioning

confidence: 81%

The importance of arginine codons AGA and AGG for the expression in E. coli of triosephosphate isomerase from seven different species

Aguirre-López

Cabrera

Gómez‐Puyou

et al. 2017

Biotechnology Reports

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“…Production levels of rGH using pCold-1, 8.0%-12.0% were comparable to that of studies using other vectors, such as pET; 5.0%-15.0% (Sekine et al, 1985;Sato et al, 1988;Tsai et al, 1995). Nucleotide sequences of the three fish mGH used in this study were different; the homology between giant gouramy versus (Seetharam et al, 1988), bacterial culture system, and construction of vector expression used. In addition, variation in fish model used in rGH bioactivity analysis may be also taken into account.…”

Section: Discussionmentioning

confidence: 99%

Production and Bioactivity Potential of Three Recombinant Growth Hormones of Farmed Fish

Alimuddin

Lesmana

Sudrajat

et al. 2010

Indonesian Aquaculture Journal

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This study was aimed to produce recombinant growth hormone (rGH) from giant grouper (Epinephelus lanceolatus), giant gouramy (Osphronemus gouramy) and common carp (Cyprinus carpio) and compare their bioactivity potential by means of inducing the growth hormone of juvenile Nile tilapia (Oreochromis niloticus) as the model. DNA fragment encoding mature GH protein of giant grouper (El-mGH), giant gouramy (Og-mGH) and common carp (Cc-mGH) was amplified by PCR method. The purified PCR products were ligated to pCold-1 to generate pCold/El-mGH, pCold/OgmGH, and pCold/Cc-mGH protein expression vector, respectively. Each of the expression vectors was transformed into the Escherichia coli BL21. E. coli BL21 was cultured using 2xYT medium and protein production was induced by cold shock at 15±1 o C for overnight. The inclusion bodies of E. coli transformants containing protein expression vector were isolated by sonication method, and rGH production was analyzed by SDS-PAGE. Juvenile of Nile tilapia of average body weight of 12.41±3.28 g was intramuscularly injected once a week for 4 weeks with 1 μg inclusion body containing rGH per gram fish body weight. The result showed that rGH in molecular weight of about 25 kDa was obtained. Fish injected with rGH of El-mGH, Cc-mGH and Og-mGH grew 20.94%, 18.09%, and 16.99% faster, respectively, compared with the control. This result indicated that the three rGH produced in E. coli possessed biological activity when tested on Nile tilapia and further research is needed to find its effect on the growth of other aquaculture fish species. KEYWORDS: bioactivity, recombinant protein, growth hormone, Nile tilapiaProduction and bioactivity potential of three recombinant ... (Alimuddin)

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“…Expression of genes containing rare codons reduces the level of expressed proteins. In addition, several other effects including frame shifts (Spanjaard et al, 1990), premature truncation, and amino acids misincorporation (Seetharam et al, 1988) are observed. The Flp encoding gene is very rich in codons having either A or T in the wobble position (253 codons out of 424) suggesting this gene to be not efficiently expressed in high-GC organisms.…”

Section: Discussionmentioning

confidence: 99%

Marker removal from actinomycetes genome using Flp recombinase

Fedoryshyn¹,

Petzke²,

Welle³

et al. 2008

Gene

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Mistranslation in IGF-1 during over-expression of the protein in Escherichia coli using a synthetic gene containing low frequency codons

Cited by 68 publications

References 10 publications

The importance of arginine codons AGA and AGG for the expression in E. coli of triosephosphate isomerase from seven different species

The importance of arginine codons AGA and AGG for the expression in E. coli of triosephosphate isomerase from seven different species

Production and Bioactivity Potential of Three Recombinant Growth Hormones of Farmed Fish

Marker removal from actinomycetes genome using Flp recombinase

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