Mitigative effects of Eleutheroside E against the mechanical barrier dysfunction induced by soybean agglutinin in IPEC‐J2 cell line

The genus Polygonatum is an essential resource for traditional Chinese medicine (TCM). To investigate the molecular mechanism of lignan biosynthesis in Polygonatum sibiritumRed and Polygonatum kingianum var. grandifolium, we performed transcriptomic and metabolomic analyses of these two plants. Seventeen lignans were detected a total of 17 lignans. Among them,Cycloolivil-6-O-glucoside,Matairesinol-4,4'-di-O-glucoside,5'-Methoxyisolariciresitnol-9'-O-glucoside,Syringaresinol,Syringaresinol-4'-O-glucoside,Isolariciresinol-9'-O-glucoside, dihydrodehydrodiconiferyl alcohol-4-O-glucoside, Pinoresinol and Eleutheroside E were reported in the genus Polygonatum for the first time. Transcriptome sequencing identified 75 genes related to the lignin biosynthesis pathway belonging to 12 classes of transcription factor families, among which genes from the SET, C2H2, and GARP-G2-like families were dominant in lignin biosynthesis. In addition, a family of transcription factors such as SET, GARP-G2-like, C2H2, Trihelix, SWI/SNF-BAF60b, PHD, FAR1, C3H, AP2/ERF-ERF, and BES1 were found to have essential roles in lignin biosynthesis by combined metabolome and transcriptome analyses. This study lays the foundation for an in-depth understanding of Polygonatum lignan biosynthesis and metabolic mechanisms. This has significant reference value for the future cloning of critical genes for lignans and the selection and breeding of Polygonatumgermplasm resources with high lignan content.

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Integrated Multi-Omics Analysis Reveals the Regulatory Mechanisms of Lignans Biosynthesis in the Genus Polygonatum

Shi,

Wan,

Lan

et al. 2024

Preprint

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By integrating metabolomic and transcriptomic analyses, our study unravels the regulatory mechanisms underlying the biosynthesis of lignans in the plant genus Polygonatum. As a perennial herb of the Liliaceae family, Polygonatum boasts rhizomes that are rich in polysaccharides, saponins, flavonoids, sterols, and amino acids—micronutrients that contribute to its essence-nourishing, antioxidant, and anti-aging properties. These rhizomes have a positive impact on memory enhancement, blood glucose and lipid reduction, and immune system strengthening. Lignans, as secondary metabolites in plants, play a pivotal role in plant defense against pests and stress, and exhibit a range of pharmacological activities, including anti-tumor, anti-HIV, anti-inflammatory, hepatoprotective, and antioxidant effects.Focusing on Polygonatum sibiricum Red and Polygonatum kingianum var. grandifolium, our research delves into the transcriptional and metabolic mechanisms of lignan biosynthesis. We discovered that transcription factor families such as GARP-G2-like and SET may be crucial in regulating the lignan synthetic pathway within the rhizomes of Polygonatum. Additionally, we identified 17 lignans, with significant differences in the content of nine, particularly the marked expression variation of Cycloolivil-6-O-glucoside between the two Polygonatum species.Our findings not only fill a gap in the field but also offer guidance for molecular breeding, underscoring the significance of lignans in Polygonatum and providing theoretical support for enhancing the plant's stress tolerance and resistance to pests and diseases. We recommend further in-depth exploration of the metabolic and regulatory mechanisms of Polygonatum lignans to inform the development of new Polygonatum varieties with high quality and lignan content.

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Mitigative effects of Eleutheroside E against the mechanical barrier dysfunction induced by soybean agglutinin in IPEC‐J2 cell line

Cited by 3 publications

References 37 publications

The protective effect of Eleutheroside E against the mechanical barrier dysfunction triggered by lipopolysaccharide in IPEC-J2 cells

The protective effect of Eleutheroside E against the mechanical barrier dysfunction triggered by lipopolysaccharide in IPEC-J2 cells

Integrative analysis of the metabolome and transcriptome revealed the regulatory mechanisms of lignan synthesis in Polygonatum

Integrated Multi-Omics Analysis Reveals the Regulatory Mechanisms of Lignans Biosynthesis in the Genus Polygonatum

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