“…Therefore, visualizing the mitochondrial microenvironment using appropriate methods is of paramount importance for the diagnosis and treatment of related diseases. [105][106][107][108] Fluorescence probes based on FRET have significant advantages, including strong biological specificity, non-invasiveness, high spatiotemporal resolution, real-time live-cell imaging, and high sensitivity and accuracy for monitoring the microenvironment. Therefore, they have broad application prospects in biological and clinical research.…”