Encyclopedia of Life Sciences 2010
DOI: 10.1002/9780470015902.a0002601.pub2
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Mitochondria: Methods for Preparation

Abstract: Mitochondria are the main energy providers of the cell. Procedures developed for the isolation of pure and intact mitochondria have contributed considerably to the understanding of the complex biochemical functions of mitochondria, as they permit the application of detailed biochemical and biophysical techniques to the investigation of this organelle. This article describes the principles underlying the procedures for the isolation of mitochondria from a large variety of tissues and the techniques for assessin… Show more

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Cited by 2 publications
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“…This is because IMF mitochondria are isolated in the presence of proteolytic enzymes, which digest nonmitochondrial proteins and lower the contamination of the mitochondrial samples with such proteins. Percoll density gradient centrifugation, extra sample washes, and filtering of the mitochondrial samples through cheese cloth are recommended approaches (91,98) to decrease contamination of mitochondrial samples with nonmitochondrial tissue material and before performing functional measurements. Ultimately, the exact procedures employed to purify isolated mitochondria depend on the study objectives/ downstream applications (i.e., function, Western blotting, proteomics) (91).…”
Section: Techniques Employed To Study Subpopulations Of Skeletal Muscle Mitochondria and Methodological Considerationsmentioning
confidence: 99%
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“…This is because IMF mitochondria are isolated in the presence of proteolytic enzymes, which digest nonmitochondrial proteins and lower the contamination of the mitochondrial samples with such proteins. Percoll density gradient centrifugation, extra sample washes, and filtering of the mitochondrial samples through cheese cloth are recommended approaches (91,98) to decrease contamination of mitochondrial samples with nonmitochondrial tissue material and before performing functional measurements. Ultimately, the exact procedures employed to purify isolated mitochondria depend on the study objectives/ downstream applications (i.e., function, Western blotting, proteomics) (91).…”
Section: Techniques Employed To Study Subpopulations Of Skeletal Muscle Mitochondria and Methodological Considerationsmentioning
confidence: 99%
“…Percoll density gradient centrifugation, extra sample washes, and filtering of the mitochondrial samples through cheese cloth are recommended approaches (91,98) to decrease contamination of mitochondrial samples with nonmitochondrial tissue material and before performing functional measurements. Ultimately, the exact procedures employed to purify isolated mitochondria depend on the study objectives/ downstream applications (i.e., function, Western blotting, proteomics) (91). Although such approaches increase the purity of the mitochondrial preparations, they do result in loss of mitochondria, which can be of particular concern when mitochondria are isolated from small tissue samples collected during muscle biopsy in human subjects.…”
Section: Techniques Employed To Study Subpopulations Of Skeletal Muscle Mitochondria and Methodological Considerationsmentioning
confidence: 99%
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