Mitochondria-specific phosphorescent imaging and tracking in living cells with an AIPE-active iridium(iii) complex

Chen, Yu; Qiao, Liping; Yu, Baoxian; Li, Guanying; Liu, Chunyuan; Ji, Liang‐Nian; Chao, Hui

doi:10.1039/c3cc46957c

Cited by 78 publications

(50 citation statements)

References 30 publications

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“…The bond lengths of IreN (ppy) (2.027(5) Å and 2.045(5) Å) were longer than IreC (ppy) (2.010(6) Å and 2.026(7) Å), but shorted than IreN (dmn) (2.140(5) Å and 2.151(5) Å), constructing a distorted octahedral structure. It was similar with the reported crystal structures of cyclometalated Ir(III) complexes [Ir(C ∧ N) 2 (N ∧ N)] þ [27,28].…”

Section: Synthesis and Characterizationsupporting

confidence: 89%

“…In previous work [27,28], we have found that some iridium(III) complexes posse high specificity for mitochondria, superior photo-stability and can be used for mitochondrial imaging and tracking without damaging mitochondrial membrane. The specific mitochondrial location of these Ir(III) complexes may also be due to their positive charge that facilitates the mitochondria membrane crossing.…”

Section: Introductionmentioning

confidence: 99%

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A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo

et al. 2015

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Section: Synthesis and Characterizationsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo

et al. 2015

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“…Some cationic cytometalated Ir(III) complexes are known to target mitochondria [42,43]. To assess if a similar behavior was observed with Ir4, (Fig.…”

Section: Intracellular Sulfite Imagingmentioning

confidence: 99%

Direct imaging of biological sulfur dioxide derivatives in vivo using a two-photon phosphorescent probe

Chen

Wang

et al. 2015

Biomaterials

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Sulfur dioxide (SO2) and its derivatives sulfite and bisulfite play important roles in biological systems. However, in vivo detection of sulfite/bisulfite remains challenging.In this study, we developed a dinuclear Ir(III) complex (Ir4) as a two-photon phosphorescent probe for sulfite and bisulfite. Ir4 selectively and rapidly responded, with high sensitivity, to sulfite/bisulfite over other bio-related ions and molecules.One-photon and two-photon microscopy images revealed that Ir4 preferentially targeted mitochondria and was capable of imaging biological sulfite/bisulfite levels in vitro and in vivo. In situ sulfite generation in C. elegans was visualized by two-photon excitation real-time imaging. Finally, Ir4 was employed to monitor sulfite distribution in rat brain and other tissues. This study is the first report of the direct visualization of SO2 derivatives in vivo. These results provide new insights into the biological importance of SO2.

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“…[32] Furthermore, while there have been some recent studies of the cytotoxicity of tris(bidentate) (pyridyl)iridium(III) complexes, they have generally utilised C-metallated ligands. [33][34][35] The results of this study demonstrate that the kinetically inert (in the absence of strong light), non-organometallic, chlorido-bearing poly(pyridyl)iridium(III) complexes, Cl-Irbb n , can have significant cytotoxic properties. The anticancer activity increased with alkane chain length, Cl-Irbb 16 showing the best activity against both cell lines.…”

Section: Discussionmentioning

confidence: 99%

Differential Anticancer Activities of the Geometric Isomers of Dinuclear Iridium(III) Complexes

et al. 2015

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The anticancer activities of dinuclear iridium(III) complexes [{Ir(tpy)Cl} 2 {μ-bb n }] 4+ {Cl-Irbb n ; tpy = 2,2Ј:6Ј,2ЈЈ-terpyridine, bb n = bis[4(4Ј-methyl-2,2Ј-bipyridyl)]-1,n-alkane (n = 7, 12 and 16)} against MCF-7 and MDA-MB-231 breast cancer cell lines have been determined. The activities of the Cl-Irbb n complexes increased with increasing chain length, the ClIrbb 16 complex showing the best anticancer properties. However, while Cl-Irbb 16 was active against the metastatic MDA-MB-231 cells (3 μM), it was relatively inactive against the nonmetastatic MCF-7 line (29 μM). The three geometric isomers of Cl-Irbb 16 were isolated and characterised by NMR spectroscopy and mass spectrometry, and their anticancer activities, lipophilicities (log P) and DNA-binding abilities were

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Mitochondria-specific phosphorescent imaging and tracking in living cells with an AIPE-active iridium(iii) complex

Abstract: An AIPE-active iridium(III) complex was found to possess high specificity for mitochondria, superior photostability, low cytotoxicity, and high resistance to the loss of mitochondrial membrane potential. Thus, this complex can be used for mitochondrial imaging and tracking in living cells.

Cited by 78 publications

References 30 publications

A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo

A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo

Direct imaging of biological sulfur dioxide derivatives in vivo using a two-photon phosphorescent probe

Differential Anticancer Activities of the Geometric Isomers of Dinuclear Iridium(III) Complexes

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