Mitochondrial autonomy: Depressed protein and glycoprotein synthesis in mitochondria of SV‐3T3 cells

Myers, Marjorie W.; Bosmann, H.Bruce

doi:10.1016/0014-5793(72)80596-9

Cited by 8 publications

(3 citation statements)

References 19 publications

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“…Of particular interest is the demonstration that the replication of Venezuelan equine encephalomyelitis virus, another Togavirus, occurred within the mitochondria (9, t2). Also other studies showed correlations between viral replication and the mitoehondrion (13,14,26). Still the proportion of cardiolipin in the Rubella.…”

Section: Discussionmentioning

confidence: 95%

Phospholipid and cholesterol composition of rubella virus and its host cell BHK 21 grown in suspension cultures

Bardeletti

Gautheron

1976

Archives of Virology

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Analysis of total lipid, phospholipid and cholesterol distribution has been conducted in parallel on BHK 21/13S cells grown in suspension cultures, on purified Rubella virus and on cells infected by the virus. Extracellular virus was purified by use of a previously described procedure (3,4). A higher content of lipid and phospholipid was found in infected cells (versus control cells) which were characterized by the presence of an unidentified nonphosphorylated lipid fraction that was detected neither in the control cells nor in the purified virus. The level and the nature of phospholipids and cholesterol of BHK 21/13S cells (infected or not) were compared to those of various clones of BHK 21cells. The same phospholipids were detected in the virus and in the cells but phosphatidyl choline level was much higher than in the control cells and lower than in the infected cells, while phosphatidyl ethanolamine content was lower than in the cells (infected or not). The presence of cardiolipin (4.4 per cent), the amount of sphingomyelin (6.9 per cent) and the molar ratio of cholesterol to phospholipids (0.26) in varions seem to favor a rubella virus maturation site in the cells.

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Section: Discussionmentioning

confidence: 95%

Phospholipid and cholesterol composition of rubella virus and its host cell BHK 21 grown in suspension cultures

Bardeletti

Gautheron

1976

Archives of Virology

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“…Investigations on how virus infections influence mitochondrial function have included aspects of mitochondrial DNA synthesis (2-lo), R N A synthesis (6), protein synthesis (6,11,12), phospholipid synthesis (13), and calcium transport (14)(15)(16). However, since infection of many different cell types with several different viruses have been involved, an overview of how infection with a given virus affects mitochondria is not available.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of mitochondrial macromolecules in herpes simplex type 1 virus infected Vero cells

Lund¹,

Ziola²

1986

Biochem. Cell Biol.

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How viral infections affect host cell mitochondrial functions is largely unknown. In this study, uptake of radiolabeled precursors was used to assess how a herpes simplex virus type 1 (HSV 1) infection influences synthesis of macromolecules comprising Vero cell mitochondria. Total macromolecular synthesis in infected cells was determined for comparative purposes. Mitochondrial and total cellular DNA syntheses were approximately halved at 1-2.5 h postinfection (PI). Mitochondrial DNA synthesis in infected cells then rose to 3.5-fold that in control cells at 3-4.5 h PI. Total DNA synthesis in infected cells also rose, but more slowly, reaching threefold that for control cells at 5-6.5 h PI. Mitochondrial and total RNA synthesis in infected cells were both decreased by approximately 40% at 1-3 h PI. Over the next 4 h, total RNA synthesis in infected cells slowly continued to decrease, while that in mitochondria recovered to control levels. Synthesis of mitochondrial proteins in infected cells decreased progressively, dropping to about 60% of control levels by 5-6.5 h PI. With the metabolic inhibitors ethidium bromide and cycloheximide, it was determined that nuclear DNA and mitochondrial DNA and mitochondrial DNA directed synthesis of mitochondrial proteins were each partially inhibited in infected cells. Total cellular protein synthesis was decreased by 30% at 1-2.5 h PI and then recovered to control levels by 5-6.5 h PI. Finally, phospholipid synthesis in mitochondria from infected cells was elevated 2.3-fold at 1-5 h PI, but dropped to 14% below control levels during 4-8 h PI.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Moreover, the virus uses ATP for its own benefit. The anti-uncoupling effect was, in part, previously observed with nonpurified virus [7], Recent studies furnish proof of a close correlation between the replication of some viruses with mitochondrial respiration [14,15] localized within the inner membrane [16]. More precisely, the RNA of Venezuelan equine encephalomyelitis virus (a togavirus like rubella virus) can penetrate isolated rat liver mitochondria [17], It has also been shown that virus replication occurred within the mitochondria [18] and that upon infec tion, the mitochondria switched to production of virus-specified products as opposed to mitochondrial-specific products [19].…”

Section: Resultsmentioning

confidence: 85%

Respiration and ATP Level in BHK21/13S Cells during the Earliest Stages of Rubella Virus Replication

Bardeletti¹

1977

Intervirology

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This study is concerned with the effect of virus infection on the energy balance of the cell and the possible involvement of mitochondria in virus replication. The process of rubella virus adsorption and penetration is immediately associated with a stimulation of O₂ uptake. Shortly afterwards, ATP levels decline, possibly because of increased ATP utilization. Virus infection appears to protect mitochondria against the uncoupling effect of dinitrophenol.

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Mitochondrial autonomy: Depressed protein and glycoprotein synthesis in mitochondria of SV‐3T3 cells

Cited by 8 publications

References 19 publications

Phospholipid and cholesterol composition of rubella virus and its host cell BHK 21 grown in suspension cultures

Phospholipid and cholesterol composition of rubella virus and its host cell BHK 21 grown in suspension cultures

Synthesis of mitochondrial macromolecules in herpes simplex type 1 virus infected Vero cells

Respiration and ATP Level in BHK21/13S Cells during the Earliest Stages of Rubella Virus Replication

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