Mitochondrial Content in Euploid Blastocyst Is Associated With Blastocyst´s Day of Development

Abstract

We did not find significant differences between the percentage of euploid blastocysts in each category. We also performed a logistic regression model for implantation, in which BMI, type of cycle and standard morphology were included. The model revealed an OR 3.20 (CI95% 1.57-6.50) (p¼0.001) comparing Xtend 1 vs 5 and an OR 2.52 (CI95% 1.23-5.19) (p¼0. 012).CONCLUSIONS: There is a direct correlation between Xtend categories, percentage of viable embryos, blastocyst rate, good morphology blastocyst rate and blastocysts available for biopsy. Regarding the implantation rate total, we observed significant differences between categories, even when we distinguished between fresh and frozen embryos.IMPACT STATEMENT: Retrospective nature of this study may be a reason for caution; nevertheless, it is the largest sample size reported with this test, based in blastocyst transfer with>90% of single-embryo-transfer. Additionally, a multivariable-analysis confirmed the magnitude of the results. It confirms that morphology and automatic time-lapse classifications can be used together to increase success rate in the laboratory.